FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext9_GATCAG-_AC8KUAANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext9_GATCAG-_AC8KUAANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16258956
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN176440.10851865273514487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG41300.040.18984610
CGTCGTG41950.040.087812
GCGTCGT42000.039.72954611
GTCGCCG25500.038.99095544
GATCGGA44950.037.397661
GAGCGTC45400.036.8023539
GTCGTGT45550.036.48064413
TGGTCGC26350.036.3137942
TCGTGTA45650.035.67236714
CGTGTAG45950.035.5305415
GATCTCG32750.035.3208934
AGAGCGT47950.035.120978
ATCGGAA47400.035.0509872
GGTCGCC28250.034.4943743
GTGGTCG29050.034.45287741
TCGGAAG50350.033.5383573
AAGAGCG49350.033.4995047
CGGAAGA51100.032.4058384
TCGGTGG36550.032.1945638
ATCTCGG37250.031.8808635
Produced by FastQC (version 0.11.2)