FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext6_GCCAAT-_AC8KUAANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext6_GCCAAT-_AC8KUAANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15244075
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG47400.040.35467512
AGCGTCG47150.040.1987410
GCGTCGT47850.040.07085411
GTCGCCG30350.039.9343844
GATCGGA49700.038.0739061
GAGCGTC50750.037.2603269
GTCGTGT51600.037.15127613
TCGTGTA50950.037.0149914
GATCTCG36800.036.75312434
ATCGGAA51800.036.5357742
TGGTCGC31750.036.51136442
GGTCGCC33050.036.4063443
CGTGTAG52300.036.39208215
GTGGTCG34150.035.68443341
TCGGAAG54650.034.8894733
TCGGTGG40900.034.68604738
AGAGCGT55200.034.4565168
AAGAGCG54700.034.3276567
TCTCGGT39600.034.1559936
CGGTGGT39650.033.28330239
Produced by FastQC (version 0.11.2)