FastQCFastQC Report
Thu 10 Mar 2016
G124-G125-M1M19-NEBNext6_GCCAAT-_AC8KUAANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext6_GCCAAT-_AC8KUAANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15244075
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC401180.2631711009031378TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT51750.039.3887142
GATCGGA53300.039.2490431
CGTATGC53150.039.1360944
CGCCAAT53350.038.9493133
TCGTATG53350.038.78444343
CGTCTGA54900.038.44405416
TCTCGTA48650.038.37552341
GTCACGC54900.038.13076829
ACGCCAA55000.037.94062432
TATCTCG50250.037.1973839
ACGTCTG57500.036.85881815
ATCGGAA57050.036.8450362
ACACGTC58950.036.0265113
ATCTCGT52450.035.97255340
AGTCACG58900.035.5798628
TCGGAAG59250.035.513713
CACGCCA58900.035.42854731
TCACGCC60150.034.94864330
CGGAAGA61450.034.2422684
GCACACG66000.032.29050411
Produced by FastQC (version 0.11.2)