FastQCFastQC Report
Tue 6 Oct 2015
G113-M4_GCCAATAT_AC67REANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG113-M4_GCCAATAT_AC67REANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22218481
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT21500.028.95846611
AGCGTCG24200.025.72756210
GTCGCCG25950.024.41503144
CGTCGTG25700.024.13931712
GGTCGCC28550.022.19184543
CGTGTAG28750.021.8073215
TCGTGTA29750.021.59191714
GATCGGA30500.021.0328141
ATCTCGG31150.020.83274735
GATCTCG31200.020.6583534
GTGGTCG31800.020.54624641
TCTCGGT33150.019.97469936
TGGTCGC32300.019.9557642
TATCGCG5750.019.89381632
GAGCGTC33250.018.8573579
GTCGTGT34650.018.41211713
ATCGGAA34550.017.9576762
AGAGCGT35600.017.42728
CTCGGTG43150.014.88693137
AAGAGCG43850.014.5497977
Produced by FastQC (version 0.11.2)