FastQCFastQC Report
Tue 6 Oct 2015
G113-M3_ACAGTGAT_AC67REANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG113-M3_ACAGTGAT_AC67REANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24801362
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC345780.13941976251142982TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG51750.034.61586843
CGTATGC53050.033.56043644
CTCGTAT52450.033.15146642
GATCGGA55850.032.298561
ATCGGAA62800.028.5903472
TCTCGTA60450.028.3280941
CGTCTGA66750.027.3355616
GATCTCG62700.027.17266339
ATCTCGT63350.027.06687740
ACACGTC67050.026.9127113
ACGTCTG70100.025.88680315
CACGTCT77950.023.46289614
CACACGT80950.022.45427712
TCGGAAG84650.022.2230593
GCACACG83150.021.8866111
CGGAAGA87500.021.2731784
AGTGATC87200.020.09277336
GAACTCC120650.015.32646521
GTGATCT114350.015.20681837
GTCACAC130400.014.27912929
Produced by FastQC (version 0.11.2)