FastQCFastQC Report
Tue 6 Oct 2015
G113-M1_TTAGGCAT_AC67REANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG113-M1_TTAGGCAT_AC67REANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38837188
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1244650.32047891829861624Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT168550.038.8177511
GTCGCCG168750.038.14483644
AGCGTCG171650.037.57839610
CGTCGTG175950.037.27144612
CGTGTAG178000.036.47091315
GGTCGCC176200.036.34492543
TCGTGTA180700.036.2668414
GTGGTCG183250.035.69065541
GTCGTGT188800.035.4221713
TGGTCGC184000.035.15062742
GATCGGA188550.034.888031
GATCTCG186850.034.84860634
ATCTCGG188400.034.7486335
TCTCGGT193650.034.29614336
GAGCGTC190050.034.0675169
ATCGGAA194450.033.0834242
AGAGCGT196950.032.9411778
GTAGATC207900.031.22493231
AAGAGCG204700.031.2103847
CTCGGTG214400.030.89509637
Produced by FastQC (version 0.11.2)