FastQCFastQC Report
Mon 1 Jan 2024
G220_M06_CKDL230041222-1A_HHWMJDSX7_L1_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG220_M06_CKDL230041222-1A_HHWMJDSX7_L1_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11030342
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC2751472.494455747609639Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG834330.7563954046030485No Hit
CCAGGACGAAGGGCACTCCGCCCCGGCCCCACCGGTCCCGGCCGCCGCCC418790.37967091138243947No Hit
CGCGCACGCGCCGCGTCGCGGTGGGGGCGTGTTGCGTGCGGTGTGGTGGT172200.15611483306682605No Hit
CACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCAGTTTTATCCGGT171600.15557087894464197No Hit
TTTCCCTCTCCGTCCCGGGCGTCGGCGTCGGGGAGAGCCCGTCCTCCCCG164500.14913408849879722No Hit
CCAGGACGAAGGGCACCCCGCCCCGGCCCCACCGGTCCCGGCCGCCGCCC150210.13617891448878014No Hit
TGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGGCGGGGCGG145610.13200859955203564No Hit
GGGGGACGAACCGCCCCGCCCGCCGCCCGCCGATCCTCTTCTTCCCCCCG123650.11209987868009894No Hit
TCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGCTCCGGGCCGGGACG119140.10801115686168208No Hit
CGCGGGGGCGGCGGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCC113770.10314276746813472No Hit
GCGGCCCTCCTACTCGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTC110930.10056805128979682No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)