FastQCFastQC Report
Mon 1 Jan 2024
G220_M06_CKDL230041222-1A_HHWMJDSX7_L1_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG220_M06_CKDL230041222-1A_HHWMJDSX7_L1_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11030342
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT9906378.981017995634225TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGTAT3402173.084373993118255TruSeq Adapter, Index 19 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTA1617211.4661467432288138TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTTT833070.755253100946462TruSeq Adapter, Index 19 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGTA598120.5422497326012194TruSeq Adapter, Index 19 (97% over 38bp)
CGCGGGGGCGGCGGCCGGGACCGGTGGGGCCGGGGCGGAGTGCCCTTCGT492090.4461239733092591No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGGAT338520.3068989157362483TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGTTT266750.24183293682099793TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGGAT193250.17519855685345023TruSeq Adapter, Index 19 (97% over 38bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT188760.17112796683910617Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTT185780.16842632803225865TruSeq Adapter, Index 19 (97% over 38bp)
CGCGGGGGCGGCGGCCGGGACCGGTGGGGCCGGGGCGGGGTGCCCTTCGT183220.16610545711094No Hit
TGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGCGGGGCGGT181220.16429227670365978No Hit
CCCCACCACCACACCGCACGCAACACGCCCCCACCGCGACGCGGCGCGTG164050.14872612290715917No Hit
CGCGGCTCCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGAC159280.1444016876357959No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTGT147620.13383084586135227TruSeq Adapter, Index 19 (97% over 38bp)
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGAGAGGG143300.129914376181627No Hit
CGACGCCACGCGGGGAGGACGGGCTCTCCCCGACGCCGACGCCCGGGACG143040.12967866272868056No Hit
CCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGG134500.12193638238959409No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGCGGGCGGG133450.12098446267577198No Hit
CGCGCACGCGCCGCGTCGCGGTGGGGGGGTGGGTGTGCGGTGTGGTGGTG131040.11879958028499932No Hit
GCGGCCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCTCCGGGGGCGG128000.11604354606593341No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGGTT118780.10768478438837163TruSeq Adapter, Index 19 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)