FastQCFastQC Report
Mon 1 Jan 2024
G220_M05_CKDL230041222-1A_HHWMJDSX7_L1_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG220_M05_CKDL230041222-1A_HHWMJDSX7_L1_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31466481
Sequences flagged as poor quality0
Sequence length150
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCAGTTTTATCCGGT4932321.5674838250899426No Hit
CACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGAGAG2561010.8138850988771195No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCTCCCCCGCTC2221690.7060497168399605No Hit
CCAGGACGAAGGGCACTCCGCCCCGGCCCCACCGGTCCCGGCCGCCGCCC1939560.616389230177979No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCCCCCGCTCCC1677690.5331673408284835No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCTCTCCCCCGC1484900.4718989708445631No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGAGAGGG1341560.42634573595948017No Hit
GGGGGACGAACCGCCCCGCCCGCCGCCCGCCGATCCTCTTCTTCCCCCCG1311330.4167386877484012No Hit
TTTCCCTCTCCGTCTTCGCGGGCGGCGGCGGCGCCGCCCTCCCCTCTTCC1259960.40041337955775863No Hit
CACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGAGAGAGAG1244370.3954589011716944No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTTTCTCTCTCCCCC1203740.3825467487133372No Hit
TTTCCCTCTCCGTCTTCGCGGGCGGCGGCGGCGCCGCCCTCCCCGTCTCT1195030.3797787239062417No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGCGGGCGGG1081290.34363232418648915No Hit
TGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGGCGGGGCGG1039170.33024665198501224No Hit
GCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGCTCCCCCCCACCCC928170.29497102011502335No Hit
CGGGGTGGGGGGGAGCGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGG880510.2798247443049002No Hit
AAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGA851030.2704560449578077No Hit
CCCCACCACCACACCGCACGCAACACGCCCCCCCGCCGTCCAGGTACCTA850790.270379773321332No Hit
AAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGCGG832300.2645036793278537No Hit
CCCCGTCTCTCTCAGCCGGGCGCGCCCCCCGCTCCCCCCCGCCCCGCCGG819990.26059157997362337No Hit
CCAGGACGAAGGGCACCCCGCCCCGGCCCCACCGGTCCCGGCCGCCGCCC779260.24764764766673464No Hit
CCCCGTCTCTCTCAGCCGGGCGCGCCCCCCGCGCCCCCCCGCCCCGCCGG762630.2423626588559426No Hit
CGCGGGGGCGGCGGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCC738220.23460519782939818No Hit
CACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCAGTTTTATCCGGTAA687980.2186390019271618No Hit
TTTCCCTCTCCGTCTTCGCGGGCGGCGGCGCCGCCGCCCTCTCCCACGTC660620.20994403536893752No Hit
CGCGCACGCGCCGCGTCGCGGTGGGGGCGTGTTGCGTGCGGTGTGGTGGT628150.1996251185507525No Hit
CCCCGTCTCTCTCAGCCGGGCGCGCCCTCCCTTCCCCCGCCGCCCCTCCT623210.1980551940332953No Hit
CGACCGCTCCCCGCCCCCAGCGGACGCGCGCGCGACGAGACGTGGGGTGG580100.18435490133135637No Hit
GGGGGACGAACCGCCCCGGCCCCACCGGTCCCGGCCGCCGCCCCCGCGAG525370.16696179023005464No Hit
CGACGCCACGCGGGGAGGACGGGCTCTCCCCGACGCCGACGCCCGGGACG504230.16024353025049098No Hit
CGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGC487580.15495218546999265No Hit
CTCCGCGCCGGAACGCGCTAGGTACCTGGACGGCGGTGGGGGCGTGTTGC447400.14218304233002732No Hit
TGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCT435000.13824234111211864No Hit
GGACGAACCGCCCCGCCCGCCGCCCGCCGATCCTCTTCTTCCCCCCGAGC426800.13563639353253387No Hit
CGGCCGGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCTTGGTG426360.13549656219899517No Hit
CGCGGCTCCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGACACGGA423410.1345590566673153No Hit
TCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGCTCCGGGCCGGGACG406470.12917555032607556No Hit
TTTCCCTCTCCGTCCCGGGCGTCGGCGTCGGGGAGAGCCCGTCCTCCCCG399160.12685244339842133No Hit
CGCGCGGGACACGCCCGCCCGCCCCCGCCACGCAGTTTTATCCGGTAAAG393280.12498378830476786No Hit
CCCCGTCTCTCTCAGCCGGGCGCGCCCCCCTCTCCCCCCCGCCCCGCCGG391050.12427509768251493No Hit
CCCCGTCTCTCTCAGCCGGGCGCGCCCCCCGCTCCCCCCCGCCCCGCCGT386650.12287678434712798No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCTCTCTCCCCC383180.12177402360308419No Hit
CCCCACCACCACACCGCACACCCACCCCCCCACCGCGACGCGGCGCGTGC375010.11917760997805889No Hit
GACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGAGA368610.11714369967204151No Hit
CCCCGTCTCTCTCAGCCGGGCGCGCCCCCCTCTCCCCCCCGCCCCGCCGT367230.11670513776230651No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG333980.10613833812557559No Hit
CCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCGGGCGGCGGG330250.10495294977534983No Hit
GGACGGTCCCGTTCGCCACGAACGTCCGCCCCTCGCCCGTCACGCACCGC325800.10353874651569714No Hit
CTCTCCCCGCCGGCACCCTTCCCCTTCCGGACCCGCCCTCCTCCGGTCGC320050.10171140522513464No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC318580.10124424145172128Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)