FastQCFastQC Report
Mon 1 Jan 2024
G220_M05_CKDL230041222-1A_HHWMJDSX7_L1_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG220_M05_CKDL230041222-1A_HHWMJDSX7_L1_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31466481
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGAGAG5430691.7258650562164863No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGAGAGGG4034051.2820149796858442No Hit
CGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGC3361341.0682287606294456No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGCGGGCGGG3266221.0379997687062623No Hit
CGCGGGGGCGGCGGCCGGGACCGGTGGGGCCGGGGCGGAGTGCCCTTCGT2405390.7644292985923656No Hit
TGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGCGGGGCGGT1977620.628484640529076No Hit
AAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGA1908060.6063785778905496No Hit
CACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCAGTTTTATCCGGT1869090.5939939709178157No Hit
AAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGCGG1760060.5593444020638978No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTTTCTCTCTCCCCC1660560.5277234527750339No Hit
CGACCGCTCCCCGCCCCCAGCGGACGCGCGCGCGACGAGACGTGGGGTGG1315650.418111577204963No Hit
CGACGCCACGCGGGGAGGACGGGCTCTCCCCGACGCCGACGCCCGGGACG1049630.33357082414140937No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCTCCCCCGCTC1019690.32405593749107187No Hit
GCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGCTCCCCCCCACCCC1016840.3231502118079235No Hit
CCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGG995710.316435129813213No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCTCTCCCCCGC992500.31541499667535117No Hit
CGCGGGGGCGGCGGCCGGGACCGGTGGGGCCGGGGCGGGGTGCCCTTCGT987450.31381011432450934No Hit
CTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGAGAGGGGG986250.31342875614213106No Hit
TGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCT954400.30330687438484144No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCTCCCGCTCCC854740.2716350773383271No Hit
TCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGAGAGGGG850600.2703193916091221No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCCCCCGCTCCC791490.25153432314213975No Hit
GACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGAGA763640.242683635326111No Hit
CTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGCGGGCGGGCG747050.23741135845473157No Hit
CCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCCGCCGCCCCC733500.2331051889787104No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCTCTCCCGCTC706940.2246644612087383No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT704270.22381593925294663TruSeq Adapter, Index 7 (97% over 36bp)
TAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGAGAGGGGGG647870.20589210468116853No Hit
CCCCACCACCACACCGCACGCAACACGCCCCCACCGCCGTCCAGGTACCT630370.20033063118815225No Hit
GACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGCGGGC629590.20008274836960638No Hit
TCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGCGGGCGGGC618540.19657107510687327No Hit
CTCCGCGCCGGAACGCGCTAGGTACCTGGACGGCGGGGGGGCGTGTTGCG610960.19416216258818392No Hit
CGCGGCTCCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGAC609220.19360919322373543No Hit
CGAGAGGGACGACGGAGGCGACGGGGGGTGGGAGAGCGGTCGCGCCGTGG596330.1895127707480223No Hit
GGGGGACGAACCGCCCCGCCCCGCCGCCCGCCGATCCTCTTCTTCCCCCC583140.18532100872671464No Hit
CGCGGGGGCGGCGGCCGGGACCGGTGGGGCCGGGGCGGTTCGTCCCCCAG528410.16792789762541288No Hit
CCCCACCACCACACCGCACGCAACACGCCCCCACCGCGACGCGGCGCGTG527580.16766412488260127No Hit
CGCGCACGCGCCGCGTCGCGGTGGGGGGGTGGGTGTGCGGTGTGGTGGTG527190.16754018347332833No Hit
CGACGCCGCCGAGAACCGCCCCCGCGCCCGCTCGCTCCCTCCCGTCCGCC513020.16303697893641173No Hit
CCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGAGAGG495680.1575263532010459No Hit
GCGGCCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCTCCGGGGGCGG483140.1535411601951931No Hit
CGCCCCCTCCGGGGAGGAGGAGGAGGGGCGGCGGGGGAAGGGAGGGCGCG460610.14638116032104131No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCTCTCTCCCCC446770.14198282928427872No Hit
CCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGCGGGCGG437020.1388842940524554No Hit
TTTCCCTCTCCGTCTTCGCGGGCGGCGGCGGCGCCGCCCTCCCCGTCTCT432230.13736203930779547No Hit
TAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGCGGGCGGGCGT430500.13681224792820018No Hit
TCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGAC412750.1311713248138551No Hit
CCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGCCAGGTGGG402720.12798380600614348No Hit
CAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGCGGACGTTCG401230.12751028626302383No Hit
CCGCCCCCAGCGGACGCGCGCGCGACGAGACGTGGGGTGGGGGGGAGCGA399500.1269604948834285No Hit
TGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGCGGGGAAGA389590.1238111118939547No Hit
CGGGGTGGGGAGGGAGCGAGCGGCGCGCGCGGGGTCGGCGGCGATGTCGG366610.11650810270141107No Hit
TGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGGCGGGGCGG361570.11490639833542239No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCTCTCTCCCGC356330.11324113427237065No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTTTCTCTCCCCCGC353220.11225278098304034No Hit
GGACGGTCCCGTTCGCCACGAACGTCCGCCCCTCGCCCGTCGCGCACCGC336550.1069550802328357No Hit
CGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGAGAGAG326950.10390421477380962No Hit
CGACCGCTCCCCGCCCCCAGCGGACGCGCGCGCGACGAGTAGGAGGGCCG315420.10023999823812521No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)