FastQCFastQC Report
Mon 1 Jan 2024
G220_M04_CKDL230041222-1A_HHWMJDSX7_L1_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG220_M04_CKDL230041222-1A_HHWMJDSX7_L1_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28726611
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGACGAACCGCCCCGCCCGCCGCCCGCCGATCCTCTTCTTCCCCCCG1849510.6438316026906202No Hit
CCAGGACGAAGGGCACTCCGCCCCGGCCCCACCGGTCCCGGCCGCCGCCC1363100.47450776563932306No Hit
CACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGAGAG1326130.46163816539305663No Hit
TGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGGCGGGGCGG1242370.432480531727185No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCCCCCGCTCCC1222910.42570632505170897No Hit
GGGGGACGAACCGCCCCGGCCCCACCGGTCCCGGCCGCCGCCCCCGCGAG1103560.3841594819521175No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCTCCCCCGCTC1077060.374934585914085No Hit
CGCGCACGCGCCGCGTCGCGGTGGGGGCGTGTTGCGTGCGGTGTGGTGGT933030.3247964056741674No Hit
CACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCAGTTTTATCCGGT929440.3235466933429773No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC908950.3164139341045138Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG869250.30259399551168775No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTTTCTCTCTCCCCC845130.29419759957065594No Hit
TTTCCCTCTCCGTCTTCGCGGGCGGCGCCGCCGCCCCCCTCCCACGTCCG818480.2849204871399553No Hit
CGCGGGGGCGGCGGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCC789320.2747696204052751No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCTCTCCCCCGC725650.2526055022640854No Hit
TTTCCCTCTCCGTCTTCGCGGGCGGCGGCGGCGCCGCCCTCCCCGTCTCT670960.2335674054972931No Hit
CCAGGACGAAGGGCACCCCGCCCCGGCCCCACCGGTCCCGGCCGCCGCCC554150.19290475998021486No Hit
CCCCACCACCACACCGCACACCCACCCCCCCACCGCGACGCGGCGCGTGC551280.19190568633383173No Hit
CGGCCGGGGAGCGGAGTCCGCGGTGGAGCCGCCGCAGGTGCAGATCTTGG541760.18859168594582912No Hit
CCCCACCACCACACCGCACGCAACACGCCCCCCCGCCGTCCAGGTACCTA532710.18544129692151992No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGAGAGGG526500.18327953826506024No Hit
CGGGGTGGGGGGGAGCGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGG524180.1824719247251268No Hit
CGCGGGGGCGGTCGGCGGGCGGCGGGGCGGGGCGGTTCGTCCCCCAGATC519780.18094024387352897No Hit
CGGCCGGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCTTGGTG509840.17748003758605566No Hit
CACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGAGAGAGAG482330.16790355117072459No Hit
TTTCCCTCTCCGTCTTCGCGGGCGGCGGCGCCGCCGCCCTCTCCCACGTC446770.15552478501553837No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGCGGGCGGG417780.14543309685921532No Hit
TTTCCCTCTCCGTCTTCGCGGGCGGCGGCGGCGCCGCCCTCCCCTCTTCC411350.14319475416017574No Hit
GCCGCCCCGGAGCCCGCGCCGCGCGCCGACCCCCGACCCGCCCCCCGACG407710.14192763636476297No Hit
GGACGAACCGCCCCGCCCGCCGCCCGCCGATCCTCTTCTTCCCCCCGAGC379710.13218057640004943No Hit
TCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGCTCCGGGCCGGGACG371750.12940962649579515No Hit
GGGGGACGAACCGCCCCGCCCCGCCGCCCGCCGATCCTCTTCTTCCCCCC350750.12209933152225996No Hit
TTTCCCTCTCCGTCTTCGCGGGCGGCGACGTCGGCTACCCACCCGACCCG348350.12126386923957025No Hit
CGACGCCGCCGAGAGCCGCCCCCGCGCCCGCTCGCTCCCTCCCGTCCGCC347850.1210898145973432No Hit
TACCACCAAGATCTGCACCTGCGGCGCCTCCACCGCGGACTCCGCTCCCC329370.11465675502063226No Hit
CGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTCCGGGGCGGCAGATC318360.11082407179879311No Hit
CGACGCCGCCGAGAACCGCCCCCGCGCCCGCTCGCTCCCTCCCGTCCGCC314770.10957435946760306No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)