FastQCFastQC Report
Mon 1 Jan 2024
G220_M04_CKDL230041222-1A_HHWMJDSX7_L1_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG220_M04_CKDL230041222-1A_HHWMJDSX7_L1_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28726611
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT6438372.2412563737504576TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTAT2756260.9594796963693351TruSeq Adapter, Index 8 (97% over 36bp)
CACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAGAGAG2485380.8651838533964205No Hit
TGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGCGGGGCGGT2294100.7985975094660487No Hit
CGCGGGGGCGGCGGCCGGGACCGGTGGGGCCGGGGCGGAGTGCCCTTCGT1559360.542827693806276No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGAGAGGG1178070.4100971047367892No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTTTCTCTCTCCCCC1170560.4074828040105392No Hit
CGCGGGGGCGGCGGCCGGGACCGGTGGGGCCGGGGCGGTTCGTCCCCCAG1110850.38669719863578755No Hit
CGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGC960160.3342406105614059No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGCGGGCGGG922200.32102638212352996No Hit
GGGGGACGAACCGCCCCGCCCCGCCGCCCGCCGATCCTCTTCTTCCCCCC916410.319010829366541No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTTT886700.308668502525411TruSeq Adapter, Index 8 (97% over 36bp)
CCCCACCACCACACCGCACGCAACACGCCCCCACCGCGACGCGGCGCGTG881280.30678175020367004No Hit
CCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCCGCCGCCCCC819950.28543220778810285No Hit
CGCGCACGCGCCGCGTCGCGGTGGGGGGGTGGGTGTGCGGTGTGGTGGTG708690.24670156879974459No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCCCCCGCTCCC690780.24046693151517246No Hit
CGACGCCGCCGAGAACCGCCCCCGCGCCCGCTCGCTCCCTCCCGTCCGCC682800.23768901942522908No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCTCCCCCGCTC667740.23244649360135103No Hit
GCGGCCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCTCCGGGGGCGG619280.21557711767670748No Hit
CGCGGGGGCGGCGGCCGGGACCGGTGGGGCCGGGGCGGGGTGCCCTTCGT616730.21468943900134965No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCTCTCCCCCGC602670.20979502246192563No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCTCTCTCTCCCGCTCCC600000.2088655706724333No Hit
CCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGG594180.2068395746369107No Hit
AAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGA529230.1842298766116198No Hit
TGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCT512360.17835727298287987No Hit
TGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGGCGGGGCGG510280.17763320567121546No Hit
CGGCCGGGGAGCGGAGTCCGCGGTGGAGGCGCCGCAGGTGCAGATCTTGG504390.17558284198578106No Hit
CACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCAGTTTTATCCGGT496820.17294765470246387No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGGAT485180.16889566263141864TruSeq Adapter, Index 8 (97% over 36bp)
AAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGCGG460050.16014767631308824No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTA452550.1575368566796828TruSeq Adapter, Index 8 (97% over 36bp)
CCCCACCACCACACCGCACGCAACACGCCCCCACCGCCGTCCAGGTACCT418020.1455166430874843No Hit
GCCGCCCCGGAGCCCGCGCCGCGCGCCGACCCCCGACCCGCCCCCCGACG407040.14169440314417875No Hit
GGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACAACC399930.13921934613171041No Hit
GCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGCTCCCCCCCACCCC394940.1374822808022847No Hit
TCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGAC394390.13729082069583495No Hit
TACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCCGG391590.13631611469936358No Hit
TACCACCAAGATCTGCACCTGCGGCGGCTCCACCGCGGACTCCGCTCCCC385450.1341787236928157No Hit
CGCGGCTCCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGAC369790.1287273322982652No Hit
GGCGGGCCCGGGCGGAAAGGACGGGGGGTCTCCCCGGACGTGGGAGAGGG344790.1200246001869138No Hit
CGCGCCCGGCGGCCGGGAGGGACCGGCGGCCACGCGCTCTCCCTCCCCTT341730.11895938577648438No Hit
CGACACGCGCACACCAACGACACGCCCTTCTTTCCGTCGGGGGGCGGGTC338390.11779670076640784No Hit
TTTCAAGACGGGTCGGGTGGGTAGCCGACGTCGCCGCCCGCGAAGACGGA334100.11630331193609995No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTTT333700.11616406822231831TruSeq Adapter, Index 8 (97% over 36bp)
CGGGGTGGGGAGGGAGCGAGCGGCGCGCGCGGGGTCGGCGGCGATGTCGG311690.10850218287148457No Hit
TTTCCCTCTCCGTCTTCGCGGGCGGCGGCGGCGCCGCCCTCCCACGTCCG303860.10577648717420932No Hit
TGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGCGGGGAAGA290580.10115359587665945No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGGAT290390.10108745511261319TruSeq Adapter, Index 8 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)