FastQCFastQC Report
Mon 1 Jan 2024
G220_M03_CKDL230041222-1A_HHWMJDSX7_L1_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG220_M03_CKDL230041222-1A_HHWMJDSX7_L1_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12528469
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC3237872.584409954640108Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG954740.7620564013049No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)