FastQCFastQC Report
Mon 1 Jan 2024
G220_M02_CKDL230041222-1A_HHWMJDSX7_L1_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG220_M02_CKDL230041222-1A_HHWMJDSX7_L1_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19736867
Sequences flagged as poor quality0
Sequence length150
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC2618071.3264871268575706Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1666750.844485601488828No Hit
TCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGCTCCGGGCCGGGACG1662330.8422461376468716No Hit
CCAGGACGAAGGGCACTCCGCCCCGGCCCCACCGGTCCCGGCCGCCGCCC769180.38971737510315085No Hit
CGCGGCTCCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGACACGGA554040.2807132459270258No Hit
GGGGGACGAACCGCCCCGCCCGCCGCCCGCCGATCCTCTTCTTCCCCCCG548990.27815458248768665No Hit
CGCGCACGCGCCGCGTCGCGGTGGGGGCGTGTTGCGTGCGGTGTGGTGGT525650.2663289974036913No Hit
CACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCAGTTTTATCCGGT435450.22062772171490036No Hit
CCCGCCTGGGACGCCGAGCGGCCCGTCCCCCTCCGAGACGCGACCTCAGA395750.20051308041950122No Hit
CGCGGCTCCCAAACCACGCTCCCCGGACCTCACCCGGCCCGGACACGGAC381710.1933994893921107No Hit
CCCCACCACCACACCGCACGCAACACGCCCCCCCGCCGTCCAGGTACCTA365430.18515096646291432No Hit
TCTGATCTGAGGTCGCGTCTCGGTGGGGGCCGAGGGCCGTCCGGCGTCCC354440.17958270681967914No Hit
TTTCCCTCTCCGTCCCGGGCGTCGGCGTCGGGGAGAGCCCGTCCTCCCCG333280.16886165367583417No Hit
CCAGGACGAAGGGCACCCCGCCCCGGCCCCACCGGTCCCGGCCGCCGCCC318080.161160330056437No Hit
CCCCACCACCACACCGCACACCCACCCCCCCACCGCGACGCGGCGCGTGC311890.15802406734564306No Hit
TGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGGCGGGGCGG311270.15770993440853606No Hit
TTTCCCTCTCCGTCTTCGCGGGCGGCGCCGCCGCCCCCCTCCCACGTCCG296260.15010487733438138No Hit
GGGGAAGGGAGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTCCGGGGC284550.14417181815128005No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGAGAGGG282200.14298115298643901No Hit
CGCGGGGGCGGCGGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCC250510.1269249065720512No Hit
GCCGCCCCGGAGCCCGCGCCGCGCGCCGGGACCGGGGTCCGGTGCGGAGT235270.11920331631155036No Hit
CGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGCTCCGGGCCGGGACGG233490.11830145078243674No Hit
CTCCGCGCCGGAACGCGCTAGGTACCTGGACGGCGGTGGGGGCGTGTTGC213620.10823399681418534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)