FastQCFastQC Report
Mon 1 Jan 2024
G220_M02_CKDL230041222-1A_HHWMJDSX7_L1_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG220_M02_CKDL230041222-1A_HHWMJDSX7_L1_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19736867
Sequences flagged as poor quality0
Sequence length150
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT300663915.233618385329342TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCGCGTAT8007054.05690021622986TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTA1900690.9630150519836811TruSeq Adapter, Index 1 (97% over 36bp)
CGCGGCTCCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGAC1812550.9183575083117296No Hit
CGCGGGGGCGGCGGCCGGGACCGGTGGGGCCGGGGCGGAGTGCCCTTCGT834580.4228533333076622No Hit
TGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGCGGGGCGGT762060.3861099129866964No Hit
TCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGAC647820.3282283859945958No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGAGAGGG546510.27689805073925866No Hit
CCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGG522780.26487486590450243No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCGCGTA518100.2625036688953723TruSeq Adapter, Index 1 (97% over 36bp)
CCCCACCACCACACCGCACGCAACACGCCCCCACCGCGACGCGGCGCGTG494950.25077435035661944No Hit
TCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTG494910.2507540837155157No Hit
CCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGCG414320.20992186855188313No Hit
CCCGCCTGGGACGCCGGACGGCCCTCGGCCCCCACCGAGACGCGACCTCA398970.20214454502834722No Hit
CGCGCACGCGCCGCGTCGCGGTGGGGGGGTGGGTGTGCGGTGTGGTGGTG384150.194635754499435No Hit
GCGGCCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCTCCGGGGGCGG375070.19003522696890038No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGCGGGCGGG371000.1879730962366013No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTTT369790.18736003034321505TruSeq Adapter, Index 1 (97% over 36bp)
TCTGATCTGAGGTCGCGTCTCGGAGGGGGACGGGCCGCTCGGCGTCCCAG368420.18666589788541413No Hit
CGCGGGGGCGGCGGCCGGGACCGGTGGGGCCGGGGCGGGGTGCCCTTCGT354020.17936990708809053No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGGAT345690.17514937907824984TruSeq Adapter, Index 1 (97% over 36bp)
CCCCACCACCACACCGCACGCAACACGCCCCCACCGCCGTCCAGGTACCT271420.13751929320899817No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG262070.13278196585101373TruSeq Adapter, Index 1 (97% over 35bp)
CTCCGCGCCGGAACGCGCTAGGTACCTGGACGGCGGGGGGGCGTGTTGCG261900.13269583262632312No Hit
CCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCCGCCGCCCCC252740.12805477181358116No Hit
CGACGCCACGCGGGGAGGACGGGCTCTCCCCGACGCCGACGCCCGGGACG252130.12774570553675008No Hit
GGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACAACC220500.11171985908401774No Hit
CACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCAGTTTTATCCGGT205290.10401346880434467No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)