FastQCFastQC Report
Mon 1 Jan 2024
G220_M01_CKDL230041222-1A_HHWMJDSX7_L1_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG220_M01_CKDL230041222-1A_HHWMJDSX7_L1_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18987046
Sequences flagged as poor quality0
Sequence length150
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC4167372.1948490565620373Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2201471.1594589279448735No Hit
CGCGCACGCGCCGCGTCGCGGTGGGGGCGTGTTGCGTGCGGTGTGGTGGT667660.3516397442761765No Hit
TCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGCTCCGGGCCGGGACG607180.319786448086764No Hit
CCAGGACGAAGGGCACTCCGCCCCGGCCCCACCGGTCCCGGCCGCCGCCC506750.2668924908066268No Hit
GGGGGACGAACCGCCCCGCCCGCCGCCCGCCGATCCTCTTCTTCCCCCCG380070.20017331816650155No Hit
CGCGGGGGCGGCGGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCC281370.14819050841294637No Hit
TGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGGCGGGGCGG270210.14231281685418573No Hit
TTTCCCTCTCCGTCTTCGCGGGCGGCGCCGCCGCCCCCCTCCCACGTCCG264870.13950037304381102No Hit
TTTCCCTCTCCGTCCCGGGCGTCGGCGTCGGGGAGAGCCCGTCCTCCCCG261710.1378360804519039No Hit
CACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCAGTTTTATCCGGT257680.13571358072235143No Hit
CCCCACCACCACACCGCACACCCACCCCCCCACCGCGACGCGGCGCGTGC256190.13492883516477497No Hit
CCCCACCACCACACCGCACGCAACACGCCCCCCCGCCGTCCAGGTACCTA223560.11774343412872122No Hit
GCGGCCCTCCTACTCGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTC220160.1159527395678085No Hit
CCCGCCTGGGACGCCGAGCGGCCCGTCCCCCTCCGAGACGCGACCTCAGA197540.1040393539890302No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)