FastQCFastQC Report
Mon 1 Jan 2024
G220_M01_CKDL230041222-1A_HHWMJDSX7_L1_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG220_M01_CKDL230041222-1A_HHWMJDSX7_L1_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18987046
Sequences flagged as poor quality0
Sequence length150
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT274292414.446291434697109TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTTT5956403.137086200770778TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTAT5468752.880253199997514TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGGAT5145042.709763277552496TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGGTT4349602.2908250182782512TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA1679860.8847400485573164TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGGAT1295730.6824284304151367TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGGGT1253360.6601132161369389TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTGT1244070.6552204065866802TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTTT1006520.5301087910146739TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGGTT843770.4443924557827479TruSeq Adapter, Index 7 (97% over 36bp)
CGCGGCTCCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGAC786810.4143930551387509No Hit
TGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGCGGGGCGGT708900.3733598159503063No Hit
CGCGGGGGCGGCGGCCGGGACCGGTGGGGCCGGGGCGGAGTGCCCTTCGT607760.3200919195118609No Hit
CCCCACCACCACACCGCACGCAACACGCCCCCACCGCGACGCGGCGCGTG588220.3098006925353212No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTT518010.2728228498524731TruSeq Adapter, Index 7 (97% over 36bp)
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGGGAGAGGG490810.2584972933651712No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGGGT403520.21252384388809084TruSeq Adapter, Index 7 (97% over 36bp)
CCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGG376280.19817722040595467No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGGT374750.19737140785354396TruSeq Adapter, Index 7 (97% over 36bp)
GCGGCCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCTCCGGGGGCGG373500.1967130642649731No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGGA361000.19012962837926448TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTA353650.1862585680784678TruSeq Adapter, Index 7 (97% over 36bp)
CGCGCACGCGCCGCGTCGCGGTGGGGGGGTGGGTGTGCGGTGTGGTGGTG314580.165681380874097No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGCGGGCGGG292930.1542788699200497No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTGT280760.1478692367417238TruSeq Adapter, Index 7 (97% over 36bp)
CCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCCGCCGCCCCC265100.13962150826410807No Hit
CCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGCG235430.12399506484579012No Hit
CGACCGCTCCCCGCCCCCAGCGGACGCGCGCGCGACGAGTAGGAGGGCCG221610.11671641813055068No Hit
TCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGAC212200.11176040759578927No Hit
GGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACAACC203860.10736793917284447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)