FastQCFastQC Report
Thu 18 May 2023
G203M6_CKDL230013446-1A_H5NJJDSX7_L3_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG203M6_CKDL230013446-1A_H5NJJDSX7_L3_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37933611
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGCCACAGTGTAGATC523010.1378750891920097Illumina Single End PCR Primer 1 (97% over 34bp)
TGACCTAGACTAATCTTCTAGGTCAAGATCGGAAGAGCGTCGTGTAGGGA515960.1360165790702077Illumina Single End PCR Primer 1 (100% over 25bp)
TGACCTAGTAGACTAATCTTCTAGGTCAAGATCGGAAGAGCGTCGTGTAG431920.11386208394449979Illumina Single End PCR Primer 1 (100% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG420120.11075138615198012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)