FastQCFastQC Report
Thu 18 May 2023
G203M6_CKDL230013446-1A_H5NJJDSX7_L3_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG203M6_CKDL230013446-1A_H5NJJDSX7_L3_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37933611
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTCGTAT4947601.30427867781952TruSeq Adapter, Index 10 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTCGTA469300.1237161418669053TruSeq Adapter, Index 10 (97% over 37bp)
TGACCTAGAAGATTAGTCTAGGTCAAGATCGGAAGAGCACACGTCTGAAC468050.12338661879566382Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACCTAGAAGATTAGTCTACTAGGTCAAGATCGGAAGAGCACACGTCTG421120.1110150046089733Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCGCGTAT392390.10344124633955885TruSeq Adapter, Index 10 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)