FastQCFastQC Report
Thu 18 May 2023
G203M5_CKDL230013446-1A_H5NJJDSX7_L3_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG203M5_CKDL230013446-1A_H5NJJDSX7_L3_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25107791
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTTACCGTGTAGATC685790.2731383258686517Illumina Single End PCR Primer 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG527040.20991093959639862No Hit
TGACCTAGACTAATCTTCTAGGTCAAGATCGGAAGAGCGTCGTGTAGGGA424950.16925025383555248Illumina Single End PCR Primer 1 (100% over 25bp)
TGACCTAGTAGACTAATCTTCTAGGTCAAGATCGGAAGAGCGTCGTGTAG315690.12573388077031547Illumina Single End PCR Primer 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)