FastQCFastQC Report
Thu 18 May 2023
G203M5_CKDL230013446-1A_H5NJJDSX7_L3_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG203M5_CKDL230013446-1A_H5NJJDSX7_L3_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25107791
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATCACGATCTCGGTT1973500.7860110035167969TruSeq Adapter, Index 4 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATCACGATCTCGTTT1092210.435008400380583TruSeq Adapter, Index 4 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATCACGATCTCGTAT778330.30999541138445835TruSeq Adapter, Index 4 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATCACGATCGCGGTT490800.19547717280265714TruSeq Adapter, Index 4 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATCACGATCTCGGAT484510.19297197431665733TruSeq Adapter, Index 4 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATCACGATCTCGGGT421670.1679438864215494TruSeq Adapter, Index 4 (97% over 39bp)
TGACCTAGAAGATTAGTCTAGGTCAAGATCGGAAGAGCACACGTCTGAAC384680.1532114075666792Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACCTAGAAGATTAGTCTACTAGGTCAAGATCGGAAGAGCACACGTCTG307180.12234449458337456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATCACGATCGCGTTT300660.11974769106529524TruSeq Adapter, Index 4 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)