FastQCFastQC Report
Thu 18 May 2023
G203M2_CKDL230013446-1A_H5NJJDSX7_L3_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG203M2_CKDL230013446-1A_H5NJJDSX7_L3_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14602507
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACCAGGTGTGTAGATC1478621.0125795522645529Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG483080.33081990647222426No Hit
TTTTAAAGCTGTTGGAAACAGCTTTAAAAAGATCGGAAGAGCGTCGTGTA392110.2685223845467083Illumina Single End PCR Primer 1 (100% over 21bp)
TGACCTAGACTAATCTTCTAGGTCAAGATCGGAAGAGCGTCGTGTAGGGA177220.1213627221681866Illumina Single End PCR Primer 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)