FastQCFastQC Report
Thu 18 May 2023
G203M2_CKDL230013446-1A_H5NJJDSX7_L3_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG203M2_CKDL230013446-1A_H5NJJDSX7_L3_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14602507
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCTCGGTT1442220.9876523257273564TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCTCGGT602060.4122990661808962TruSeq Adapter, Index 9 (97% over 36bp)
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG489130.3349630306631594No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCTCGTTT443660.30382454190913927TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCTCGGGT429640.29422345080882345TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCGCGGTT336470.23041933826842198TruSeq Adapter, Index 9 (97% over 36bp)
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG326390.22351641399658292No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCTCGTT218300.14949487783159426TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCTCGGG185510.12703982953064155TruSeq Adapter, Index 9 (97% over 36bp)
TGACCTAGAAGATTAGTCTAGGTCAAGATCGGAAGAGCACACGTCTGAAC161250.1104262439319495Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)