FastQCFastQC Report
Thu 18 May 2023
G203M13_CKDL230013446-1A_H5NJJDSX7_L3_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG203M13_CKDL230013446-1A_H5NJJDSX7_L3_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12739868
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCTCGGTT1739341.3652731723750984TruSeq Adapter, Index 9 (97% over 36bp)
TGACCTAGAAGATTAGTCTAGGTCAAGATCGGAAGAGCACACGTCTGAAC757750.5947863824020783Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACCTAGAAGATTAGTCTACTAGGTCAAGATCGGAAGAGCACACGTCTG632010.49608834251657863Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCTCGTTT516210.4051925812732126TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCTCGGGT505600.39686439451334976TruSeq Adapter, Index 9 (97% over 36bp)
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG491450.38575752904190214No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCGCGGTT389890.30603927764400696TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCTCGGT221650.17398139446970723TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCTCGGAT164530.1291457650895598TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCGCGGGT150320.11799180336876333TruSeq Adapter, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)