FastQCFastQC Report
Thu 2 Mar 2023
G199M8_CKDL230005140-1A_HT3M7DSX5_L2_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG199M8_CKDL230005140-1A_HT3M7DSX5_L2_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6238768
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT1245261.9960030570138207TruSeq Adapter, Index 8 (97% over 36bp)
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG698951.1203333735122063No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTAT488150.7824461496244131TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTA278710.44673884331008945TruSeq Adapter, Index 8 (97% over 36bp)
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG164880.2642829481718185No Hit
CTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG136680.21908171613369817No Hit
ATTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131990.21156420626636543No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG125860.2017385483800648No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTA116690.18704013356483204TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTTT72530.11625692764981804TruSeq Adapter, Index 8 (97% over 36bp)
AATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64970.10413915055023686No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)