FastQCFastQC Report
Thu 2 Mar 2023
G199M7_CKDL230005140-1A_HT3M7DSX5_L2_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG199M7_CKDL230005140-1A_HT3M7DSX5_L2_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7088002
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG639180.9017773979183414No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC568080.8014670424754394Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)