FastQCFastQC Report
Thu 2 Mar 2023
G199M6_CKDL230005140-1A_HT3M7DSX5_L2_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG199M6_CKDL230005140-1A_HT3M7DSX5_L2_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6917230
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT685760.9913794972843176TruSeq Adapter, Index 7 (97% over 36bp)
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243380.35184604241871387No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTAT132620.19172414391309817TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTTT115620.1671478322970322TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA103790.15004561074302863TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)