FastQCFastQC Report
Thu 2 Mar 2023
G199M5_CKDL230005140-1A_HT3M7DSX5_L2_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG199M5_CKDL230005140-1A_HT3M7DSX5_L2_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5915218
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC2306483.8992307637689767Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG934341.5795529429346473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)