FastQCFastQC Report
Thu 2 Mar 2023
G199M4_CKDL230005140-1A_HT3M7DSX5_L2_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG199M4_CKDL230005140-1A_HT3M7DSX5_L2_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6399952
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT1008791.5762461968464765TruSeq Adapter, Index 6 (97% over 36bp)
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG468010.7312711095333215No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA336870.5263633227249204TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTTT239560.3743153073648052TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTAT232450.3632058490438678TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGGAT172460.26947077103078276TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGGTT125940.19678272587044404TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTT119390.18654827411205585TruSeq Adapter, Index 6 (97% over 36bp)
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113860.1779075843068823No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92850.1450792130940982No Hit
CTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91930.14364170231276735No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGTA88180.13778228336712525TruSeq Adapter, Index 6 (97% over 36bp)
ATTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86130.13457913434350757No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCGCGGAT82210.12845408840566305TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGGGT69420.1084695635217264TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGGA68900.1076570574279307TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGGT65130.10176638824791187TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)