FastQCFastQC Report
Thu 2 Mar 2023
G199M3_CKDL230005140-1A_HT3M7DSX5_L2_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG199M3_CKDL230005140-1A_HT3M7DSX5_L2_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13995047
Sequences flagged as poor quality0
Sequence length150
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5982004.274369353672053No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATC1703861.2174735819036548Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT328600.23479735366376403No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG312760.22347906370017906No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT256310.18314336493475156No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG220740.15772723021223153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTT163500.11682704602564037No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGT144390.10317221514154257No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)