FastQCFastQC Report
Thu 2 Mar 2023
G199M3_CKDL230005140-1A_HT3M7DSX5_L2_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG199M3_CKDL230005140-1A_HT3M7DSX5_L2_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13995047
Sequences flagged as poor quality0
Sequence length150
%GC62

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT443938331.72110104381929TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTAT11851538.468374561371606TruSeq Adapter, Index 2 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA1662921.1882203753942377TruSeq Adapter, Index 2 (97% over 35bp)
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1234290.8819477347950313No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA1145600.8185753145380648TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGGAT1081260.7726019069460789TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTTT981110.7010408753897004TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGGAT801460.5726740324630564TruSeq Adapter, Index 2 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG642440.4590481189523694TruSeq Adapter, Index 2 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTA532600.3805632092553887TruSeq Adapter, Index 2 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTA381090.2723034799382953TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTTT337810.2413782533206212TruSeq Adapter, Index 2 (97% over 37bp)
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG314650.224829541479925No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG289950.2071804403372136No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC284860.20354343933250096TruSeq Adapter, Index 2 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTGGTAT259120.1851512181416754TruSeq Adapter, Index 2 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTATG243750.1741687612767574TruSeq Adapter, Index 2 (97% over 36bp)
CTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG199590.14261474077221747No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTGT183950.13143935851019292TruSeq Adapter, Index 2 (97% over 37bp)
ATTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG171930.12285060564641191No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)