FastQCFastQC Report
Thu 2 Mar 2023
G199M2_CKDL230005140-1A_HT3M7DSX5_L2_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG199M2_CKDL230005140-1A_HT3M7DSX5_L2_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9655264
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC811600.84057774080543Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG794720.8230950494983876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)