FastQCFastQC Report
Thu 2 Mar 2023
G199M2_CKDL230005140-1A_HT3M7DSX5_L2_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG199M2_CKDL230005140-1A_HT3M7DSX5_L2_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9655264
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1339521.3873468400242603No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1029911.066682381755693TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA408380.4229609879129147TruSeq Adapter, Index 7 (97% over 36bp)
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG360880.3737650260003248No Hit
CTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG297580.30820493359891554No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG244630.2533643823721443No Hit
ATTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG242930.2516036847879043No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGGAT165710.17162658628495295TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTAT157170.16278167018529996TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTTT133860.1386393991919848TruSeq Adapter, Index 7 (97% over 36bp)
AATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122190.12655272812840745No Hit
ATAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG116200.12034885840511456No Hit
AGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103990.10770290693242568No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)