FastQCFastQC Report
Thu 2 Mar 2023
G199M1_CKDL230005140-1A_HT3M7DSX5_L2_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG199M1_CKDL230005140-1A_HT3M7DSX5_L2_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18901933
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT285053115.080632229518537TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGTAT9980385.280084317302363TruSeq Adapter, Index 19 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTA2917741.5436199038479292TruSeq Adapter, Index 19 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGTA1160930.6141858613084704TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTTT792970.4195179403080097TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGGAT565670.2992656888583829TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGGAT523880.27715683893282234TruSeq Adapter, Index 19 (97% over 38bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTA438440.23195511273899871TruSeq Adapter, Index 12 (97% over 35bp)
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG415460.21979762598883404No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGTTT345940.18301831881427152TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG289560.15319068160912433TruSeq Adapter, Index 19 (97% over 37bp)
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG209520.11084580608766309No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)