FastQCFastQC Report
Wed 27 Jan 2021
G124-G125-M20M38-NEBNext2_CGATGT-_AC8KUAANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext2_CGATGT-_AC8KUAANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16489896
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5587343.3883415638279346Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC1039120.6301555813329569No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC501390.30405892189981065No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC455540.2762540163988906No Hit
GGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCC453580.275065409751523No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC299630.18170520905650345No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC248830.1508984653390173No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG229850.13938838668236597Illumina Single End PCR Primer 1 (98% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN229550.1392064570934832No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC195330.11845435532158602No Hit
GCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCC183770.1114440018299691No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTGGCCG180740.10960651298225288Illumina Single End PCR Primer 1 (98% over 50bp)
CGTCGTCCCCCGCGTCGTCGCCACCTCTCTTCCCCCCTCCTTCTTCCCGT165320.10025533211367736No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)