========================================================== Starting on : Mon Jul 15 11:45:08 EDT 2019 Running on node : scc-wl3 Current directory : /restricted/projectnb/waxmanlab/varsha/cufflinks Current job ID : 7712700 Current job name : cuffGFP_1343 Task index number : ========================================================== /scratch/7712700.1.w Running on host scc-wl3 Directory is /scratch/7712700.1.w Start time is Mon Jul 15 11:45:08 EDT 2019 copying... cp: cannot stat '/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/varsha/GSE125237/GFP_1343/fastq/tophat2/GFP_1343_primary_unique.bam': No such file or directory Current dir: /scratch/7712700.1.w running commands... genes.gtf genomeWithRandom.fa Warning: Could not connect to update server to verify current version. Please check at the Cufflinks website (http://cufflinks.cbcb.umd.edu). open: No such file or directory File mapped.bam doesn't appear to be a valid BAM file, trying SAM... Error: cannot open alignment file mapped.bam for reading cufflinks -o GFP_1343_cufflinks -p 4 -g genes.gtf -b genomeWithRandom.fa -u --library-type fr-secondstrand mapped.bam done running. copying... List of files in scratch genes.gtf genomeWithRandom.fa GFP_1343_cufflinks: Size of files in scratch 4.0K GFP_1343_cufflinks 89M genes.gtf 2.6G genomeWithRandom.fa 2.7G total Stop time is Mon Jul 15 11:45:40 EDT 2019