-----------------------
Start of variable list:
-----------------------
Sample_ID:
NASH_OA-NO2_3
Dataset_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/varsha/GSE126204
Sample_Labels_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/varsha/GSE126204/Scripts/00_Setup_Pipeline
Bowtie2Index_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/varsha/
GTF_Files_DIR:
/unprotected/projects/waxmanlab/routines/GTF_Files
ANNOTATION_FILE:
RefSeq_GeneBody.gtf
STRANDEDNESS:
fr-secondstrand
DISTANCE_BT_READ_PAIR:
-86
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Wed Jun 26 17:45:53 EDT 2019
Running on node : scc-ka4
Current directory : /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/varsha/GSE126204/Scripts/04_TopHat_Paired_End
Current job ID : 7076179
Current job name : Step_04_NASH_OA-NO2_3
Task index number : undefined
Parameter for multiple cores : 16
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/7076179.1.linga

Loading required modules...


Here is the Left_List:
SRR8543830_1.fastq.gz


Here is the Right_List:
SRR8543830_2.fastq.gz


List files in the scratch directory:

total 2.3G
drwx------   4 varsha97 waxmanlab 4.0K Jun 26 17:47 .
drwxrwxrwt. 10 root     root      4.0K Jun 26 17:45 ..
drwxr-xr-x   2 varsha97 waxmanlab 4.0K Jun 26 17:46 Bowtie2Index
-rw-r--r--   1 varsha97 waxmanlab  72M Jun 26 17:47 RefSeq_GeneBody.gtf
-rw-r--r--   1 varsha97 waxmanlab 1.1G Jun 26 17:46 SRR8543830_1.fastq.gz
-rw-r--r--   1 varsha97 waxmanlab 1.2G Jun 26 17:46 SRR8543830_2.fastq.gz
drwxr-xr-x   2 varsha97 waxmanlab 4.0K Jun 26 17:47 tophat2

Starting to run my commands


Starting tophat

tophat --no-coverage-search -o /scratch/7076179.1.linga/tophat2 -G RefSeq_GeneBody.gtf -p 16 --library-type fr-secondstrand -r -86 --mate-std-dev 150 Bowtie2Index/genome SRR8543830_1.fastq.gz SRR8543830_2.fastq.gz

Ending tophat


Starting samtools commands


Ending samtools commands


Starting spliced_read_counts


Ending spliced_read_counts


List files in scratch

total 2.3G
drwx------   4 varsha97 waxmanlab  20K Jun 27 00:46 .
drwxrwxrwt. 12 root     root      4.0K Jun 27 01:13 ..
drwxr-xr-x   2 varsha97 waxmanlab 4.0K Jun 26 17:46 Bowtie2Index
-rw-r--r--   1 varsha97 waxmanlab  72M Jun 26 17:47 RefSeq_GeneBody.gtf
-rw-r--r--   1 varsha97 waxmanlab 1.1G Jun 26 17:46 SRR8543830_1.fastq.gz
-rw-r--r--   1 varsha97 waxmanlab 1.2G Jun 26 17:46 SRR8543830_2.fastq.gz
-rw-r--r--   1 varsha97 waxmanlab    9 Jun 27 00:46 numunique_hits.txt
drwxr-xr-x   3 varsha97 waxmanlab 4.0K Jun 27 01:24 tophat2
==========================================================
Finished on : Thu Jun 27 01:25:14 EDT 2019
7 hours, 39 minutes and 21 seconds elapsed.
==========================================================