##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543824_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27554939
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.70366662760531	32.0	32.0	32.0	32.0	32.0
2	31.022310120156682	32.0	32.0	32.0	32.0	32.0
3	33.28236974141006	37.0	32.0	37.0	27.0	37.0
4	34.8980853124008	37.0	37.0	37.0	27.0	37.0
5	35.43901614153455	37.0	37.0	37.0	32.0	37.0
6	38.685175423542034	41.0	41.0	41.0	32.0	41.0
7	38.481819720232366	41.0	41.0	41.0	32.0	41.0
8	38.41705594049763	41.0	41.0	41.0	32.0	41.0
9	38.58627355335463	41.0	41.0	41.0	32.0	41.0
10-11	38.664019071136394	41.0	41.0	41.0	34.5	41.0
12-13	38.77473455847607	41.0	41.0	41.0	34.5	41.0
14-15	38.632457651239946	41.0	41.0	41.0	32.0	41.0
16-17	38.44031235198888	41.0	41.0	41.0	32.0	41.0
18-19	38.60500090383071	41.0	39.0	41.0	34.5	41.0
20-21	38.740673332646466	41.0	41.0	41.0	34.5	41.0
22-23	38.47622023405677	41.0	39.0	41.0	32.0	41.0
24-25	38.50821569592297	41.0	39.0	41.0	34.5	41.0
26-27	38.76204928270754	41.0	41.0	41.0	37.0	41.0
28-29	38.310029211097145	41.0	39.0	41.0	32.0	41.0
30-31	38.563767497362264	41.0	39.0	41.0	32.0	41.0
32-33	38.7627778998168	41.0	41.0	41.0	34.5	41.0
34-35	38.67829540468226	41.0	41.0	41.0	34.5	41.0
36-37	38.396702935324086	41.0	37.0	41.0	32.0	41.0
38-39	38.34455572898594	41.0	37.0	41.0	32.0	41.0
40-41	38.39194140074412	41.0	37.0	41.0	32.0	41.0
42-43	38.378103000482454	41.0	37.0	41.0	32.0	41.0
44-45	38.3591054554754	41.0	37.0	41.0	32.0	41.0
46-47	38.387981082701785	41.0	37.0	41.0	32.0	41.0
48-49	38.386276398923584	41.0	37.0	41.0	32.0	41.0
50-51	38.34325187476377	41.0	37.0	41.0	32.0	41.0
52-53	38.38487914290013	41.0	37.0	41.0	32.0	41.0
54-55	38.39391318212472	41.0	37.0	41.0	32.0	41.0
56-57	38.39281494898676	41.0	37.0	41.0	32.0	41.0
58-59	38.41548889666658	41.0	37.0	41.0	32.0	41.0
60-61	38.38919170787004	41.0	37.0	41.0	32.0	41.0
62-63	38.42359773116419	41.0	41.0	41.0	32.0	41.0
64-65	38.41550974436145	41.0	39.0	41.0	32.0	41.0
66-67	38.34016341724376	41.0	37.0	41.0	32.0	41.0
68-69	38.35796226543278	41.0	37.0	41.0	32.0	41.0
70-71	38.18571843047869	41.0	37.0	41.0	32.0	41.0
72-73	38.30204832938212	41.0	37.0	41.0	32.0	41.0
74-75	38.33642420233444	41.0	37.0	41.0	32.0	41.0
76	37.4393217159392	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10346.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	0.0
11	0.0
12	20.0
13	157.0
14	857.0
15	3233.0
16	10244.0
17	23470.0
18	39149.0
19	53087.0
20	65404.0
21	75973.0
22	87254.0
23	100215.0
24	114936.0
25	132111.0
26	152654.0
27	175850.0
28	205157.0
29	239900.0
30	283610.0
31	336533.0
32	406363.0
33	496349.0
34	616432.0
35	796616.0
36	1077352.0
37	1608811.0
38	2912050.0
39	7461526.0
40	1.0069279E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.9181035505325	19.14076498058893	13.848061672976664	31.093069795901908
2	22.511327104141014	27.355225518360974	28.740954457756057	21.39249291974195
3	21.73560161153951	28.229075666501952	27.48322692587979	22.552095796078746
4	25.497742144812484	32.515071549151294	19.22998029137227	22.75720601466395
5	24.79631290349084	34.46820652210416	20.014753933556324	20.720726640848675
6	20.222225602617964	36.661669772132974	20.82904496046952	22.287059664779544
7	20.378552482867452	21.15277592774137	33.693583071729414	24.775088517661764
8	21.33501645362633	23.281866738696664	26.1228292976204	29.26028751005661
9	22.246223854522007	23.195426510930165	27.312387180347343	27.245962454200484
10-11	24.07470517735103	30.45214417066059	21.21602697844887	24.257123673539507
12-13	23.850895029466425	24.59975774533839	25.318438466706027	26.230908758489154
14-15	22.980227194250805	27.52120418318799	25.879461293958816	23.619107328602386
16-17	23.299093564990887	27.419410789561432	25.29177187810539	23.98972376734229
18-19	23.12841526260405	27.752214051087627	25.368374980742114	23.750995705566208
20-21	23.144864628527824	27.747982805929812	25.419459845059933	23.687692720482435
22-23	23.346847509573816	27.35708983261923	25.310081047844974	23.985981609961975
24-25	23.184036485419238	27.320487204443445	25.543770875836856	23.951705434300457
26-27	22.96343037795591	27.645916284921903	25.469975650167818	23.92067768695437
28-29	23.56216617118031	26.999734381941344	25.304839926997875	24.133259519880472
30-31	23.252563362855835	27.3037588388051	25.52704423560736	23.91663356273171
32-33	23.092680133606724	27.491157577577486	25.63658542518971	23.77957686362608
34-35	23.34260673315351	27.336359879375355	25.204607228959762	24.116426158511374
36-37	23.030503505950488	27.142513949004048	25.716062976505217	24.110919568540247
38-39	22.815158760885495	27.475773891823934	25.82236319749927	23.8867041497913
40-41	23.315378749373288	27.15178298659723	25.423685076315262	24.109153187714224
42-43	22.909431969353488	27.191439240130073	25.68801887448007	24.211109916036374
44-45	23.003112015294107	27.072622130733414	25.690616104625562	24.233649749346913
46-47	23.248054809266456	26.928828772837416	25.462811627337494	24.360304790558637
48-49	23.05398583111274	26.89611086001214	25.805682902500372	24.24422040637475
50-51	22.916934645192615	27.07752266373572	25.750890143099582	24.254652547972086
52-53	23.142775055340053	26.97239199619853	25.42899369905974	24.455839249401677
54-55	23.010913900754417	26.812361039917544	25.82230020690539	24.35442485242265
56-57	22.89192567105652	27.172759966315834	25.619376781124714	24.31593758150293
58-59	23.057610302187378	27.18448314333979	25.54199111627204	24.215915438200796
60-61	23.02628947965544	26.927140033427747	25.811611790839457	24.234958696077356
62-63	22.85741254108694	27.335159105782708	25.713199689754134	24.094228663376217
64-65	23.26201875065566	27.052903139361096	25.57071472320946	24.11436338677379
66-67	23.083563228051272	27.00349151604764	25.8590488267453	24.053896429155788
68-69	22.910925408122143	27.059958633343896	26.100295690114717	23.92882026841925
70-71	23.229939166070572	27.101496631673005	25.61079405252266	24.05777014973376
72-73	22.921355774266935	26.872741267037785	25.8555595066412	24.350343452054076
74-75	22.873405307001203	23.782721717105197	27.54054235352817	25.803330622365433
76	24.19968150364959	0.0	38.449620346382375	37.35069814996804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	45469.0
1	28260.5
2	10367.5
3	9550.0
4	9417.0
5	8668.0
6	9001.0
7	11800.0
8	13521.5
9	14770.5
10	19606.5
11	48782.5
12	74373.5
13	95703.5
14	88121.5
15	47221.5
16	26413.0
17	18391.0
18	18485.5
19	20279.5
20	26258.5
21	32433.5
22	41002.0
23	56084.0
24	81624.5
25	107290.5
26	128961.5
27	162423.0
28	204204.0
29	232534.0
30	287576.0
31	380437.0
32	455946.5
33	499946.5
34	588580.0
35	704320.5
36	775016.0
37	812436.0
38	838796.0
39	906765.0
40	978184.0
41	1009787.0
42	1024531.0
43	1084050.5
44	1195327.0
45	1282346.5
46	1324537.5
47	1369393.0
48	1405176.0
49	1402951.0
50	1390315.5
51	1385287.0
52	1390614.0
53	1382795.5
54	1355263.0
55	1277049.5
56	1158679.5
57	1032952.5
58	947030.5
59	863050.5
60	745414.5
61	628049.0
62	539953.0
63	459627.5
64	366835.5
65	291578.0
66	222932.0
67	188339.0
68	163627.0
69	129737.5
70	100812.5
71	84293.5
72	76098.5
73	70249.0
74	57466.0
75	43973.0
76	36510.0
77	28949.5
78	24288.0
79	21569.0
80	20558.5
81	18716.0
82	16294.0
83	15014.0
84	13363.5
85	10218.0
86	7463.0
87	6409.0
88	5960.0
89	3787.5
90	1597.0
91	943.0
92	750.0
93	681.0
94	553.5
95	471.5
96	448.0
97	417.0
98	370.0
99	320.0
100	286.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.178013858060074
2	0.1722631285810504
3	0.03754680785176117
4	0.03867546213765888
5	0.040275901173288754
6	0.038098433097601844
7	0.0378734280631142
8	0.03940854305647347
9	0.03984403667161085
10-11	0.038406907741657496
12-13	0.037637535688248125
14-15	0.03807665841684498
16-17	0.0384813045675768
18-19	0.04002367778785502
20-21	0.03874260073665923
22-23	0.037766369216059595
24-25	0.0431193115687899
26-27	0.03996379741577363
28-29	0.042210218647190614
30-31	0.046801047173430504
32-33	0.05229189583762098
34-35	0.05790613435943371
36-37	0.002959508709933994
38-39	0.0039184492629910795
40-41	0.0010968355622127581
42-43	0.002331975956238183
44-45	0.0015766723980282587
46-47	1.9620688846414135E-4
48-49	0.003963033348244224
50-51	0.004076651665509404
52-53	0.001628945518299103
54-55	0.00968055971566834
56-57	0.004055253031779211
58-59	0.00412802024608279
60-61	0.0011108466527185604
62-63	3.3345944052648837E-4
64-65	7.184512859849501E-4
66-67	0.0052450750360544165
68-69	0.0038562817322344467
70-71	0.003866014754899511
72-73	0.0014824771204364413
74-75	2.898654442649172E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	16185.0
36	799.0
37	986.0
38	1131.0
39	1329.0
40	1511.0
41	1751.0
42	1892.0
43	2019.0
44	2018.0
45	2183.0
46	2331.0
47	2531.0
48	2944.0
49	3238.0
50	3538.0
51	3978.0
52	4241.0
53	4644.0
54	4890.0
55	5094.0
56	5668.0
57	5993.0
58	6499.0
59	7152.0
60	7718.0
61	8492.0
62	9130.0
63	9726.0
64	10473.0
65	11305.0
66	12841.0
67	13033.0
68	13501.0
69	14600.0
70	17517.0
71	45447.0
72	137306.0
73	619679.0
74	2001079.0
75	6766962.0
76	1.7761585E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.308725077609147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.11273843213334	22.89066627242811
2	12.23071896484808	7.658564351438578
3	4.605387017395128	4.325663880108435
4	2.5182524995732027	3.1537310074055775
5	1.5082572174073876	2.361080528306383
6	0.9936533454268716	1.8666011648649912
7	0.7408343480588615	1.623620525199729
8	0.5696578330192112	1.426820838584405
9	0.39733024610564976	1.1195913096306513
>10	2.7495352054324718	16.50670291755011
>50	0.29835041990350003	6.492098074583723
>100	0.23118236721651284	14.73500809533518
>500	0.028329915396696773	6.094309884831537
>1k	0.015429151677426041	8.209026067305032
>5k	2.327747041320237E-4	0.47469409491580183
>10k+	1.1026170195678893E-4	1.061820987511832
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106724	0.3873135048493484	No Hit
CCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46678	0.1693997580615221	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.258226918956344E-6	0.0
2	0.0	0.0	0.0	7.258226918956344E-6	0.0
3	0.0	0.0	0.0	7.258226918956344E-6	0.0
4	0.0	0.0	0.0	7.258226918956344E-6	0.0
5	0.0	0.0	0.0	7.258226918956344E-6	0.0
6	0.0	3.629113459478172E-6	0.0	7.258226918956344E-6	0.0
7	0.0	3.629113459478172E-6	0.0	2.5403794216347203E-5	0.0
8	0.0	3.629113459478172E-6	0.0	2.5403794216347203E-5	0.0
9	0.0	3.629113459478172E-6	0.0	2.9032907675825376E-5	0.0
10	3.629113459478172E-6	3.629113459478172E-6	0.0	3.266202113530355E-5	0.0
11	3.629113459478172E-6	7.258226918956344E-6	0.0	3.266202113530355E-5	0.0
12	3.629113459478172E-6	7.258226918956344E-6	0.0	3.266202113530355E-5	0.0
13	1.0887340378434517E-5	1.0887340378434517E-5	0.0	3.266202113530355E-5	0.0
14	1.814556729739086E-5	1.0887340378434517E-5	0.0	3.266202113530355E-5	0.0
15	2.1774680756869033E-5	1.0887340378434517E-5	0.0	3.266202113530355E-5	0.0
16	2.9032907675825376E-5	1.0887340378434517E-5	0.0	3.266202113530355E-5	0.0
17	2.9032907675825376E-5	1.0887340378434517E-5	0.0	3.266202113530355E-5	0.0
18	2.9032907675825376E-5	1.0887340378434517E-5	0.0	3.266202113530355E-5	0.0
19	2.9032907675825376E-5	1.0887340378434517E-5	0.0	3.992024805425989E-5	0.0
20	3.629113459478172E-5	1.0887340378434517E-5	0.0	3.992024805425989E-5	0.0
21	3.992024805425989E-5	1.0887340378434517E-5	0.0	4.3549361513738066E-5	0.0
22	4.3549361513738066E-5	1.0887340378434517E-5	0.0	4.3549361513738066E-5	0.0
23	5.0807588432694406E-5	1.4516453837912688E-5	0.0	4.3549361513738066E-5	0.0
24	5.4436701892172576E-5	1.4516453837912688E-5	0.0	4.3549361513738066E-5	0.0
25	6.53240422706071E-5	1.4516453837912688E-5	0.0	4.3549361513738066E-5	0.0
26	6.53240422706071E-5	1.4516453837912688E-5	0.0	4.3549361513738066E-5	0.0
27	7.258226918956344E-5	1.4516453837912688E-5	0.0	4.3549361513738066E-5	3.629113459478172E-6
28	7.621138264904161E-5	1.4516453837912688E-5	0.0	4.3549361513738066E-5	7.258226918956344E-6
29	8.709872302747613E-5	1.4516453837912688E-5	0.0	4.3549361513738066E-5	7.258226918956344E-6
30	9.435694994643247E-5	1.4516453837912688E-5	0.0	4.3549361513738066E-5	7.258226918956344E-6
31	1.0887340378434515E-4	1.4516453837912688E-5	0.0	4.3549361513738066E-5	7.258226918956344E-6
32	1.306480845412142E-4	1.4516453837912688E-5	0.0	4.3549361513738066E-5	7.258226918956344E-6
33	1.4516453837912688E-4	1.4516453837912688E-5	0.0	4.3549361513738066E-5	7.258226918956344E-6
34	1.5242276529808322E-4	1.4516453837912688E-5	0.0	4.7178474973216236E-5	7.258226918956344E-6
35	1.560518787575614E-4	1.4516453837912688E-5	0.0	5.0807588432694406E-5	7.258226918956344E-6
36	1.705683325954741E-4	1.4516453837912688E-5	0.0	6.169492881112893E-5	7.258226918956344E-6
37	1.8508478643338677E-4	1.4516453837912688E-5	0.0	6.53240422706071E-5	7.258226918956344E-6
38	2.068594671902558E-4	1.4516453837912688E-5	0.0	6.895315573008527E-5	1.0887340378434517E-5
39	2.32263261406603E-4	1.4516453837912688E-5	0.0	7.258226918956344E-5	1.0887340378434517E-5
40	2.467797152445157E-4	1.4516453837912688E-5	0.0	7.984049610851978E-5	1.0887340378434517E-5
41	2.6855439600138474E-4	1.4516453837912688E-5	0.0	8.346960956799795E-5	1.0887340378434517E-5
42	2.9032907675825376E-4	1.4516453837912688E-5	0.0	9.07278364869543E-5	1.0887340378434517E-5
43	3.193619844340791E-4	1.4516453837912688E-5	0.0	9.435694994643247E-5	1.814556729739086E-5
44	3.411366651909482E-4	1.4516453837912688E-5	0.0	1.1250251724382334E-4	1.814556729739086E-5
45	3.59282232488339E-4	1.4516453837912688E-5	0.0	1.161316307033015E-4	1.814556729739086E-5
46	3.7016957286677355E-4	1.4516453837912688E-5	0.0	1.2338985762225786E-4	1.814556729739086E-5
47	3.883151401641644E-4	1.4516453837912688E-5	0.0	1.3427719800069237E-4	1.814556729739086E-5
48	4.028315940020771E-4	1.4516453837912688E-5	0.0	1.3790631146017054E-4	1.814556729739086E-5
49	4.4638095551581514E-4	1.4516453837912688E-5	0.0	1.4516453837912688E-4	1.814556729739086E-5
50	4.572682958942497E-4	1.4516453837912688E-5	0.0	1.4879365183860505E-4	2.1774680756869033E-5
51	4.8267209011059684E-4	1.4516453837912688E-5	0.0	1.4879365183860505E-4	2.1774680756869033E-5
52	4.935594304890314E-4	1.4516453837912688E-5	0.0	1.4879365183860505E-4	2.1774680756869033E-5
53	5.153341112459004E-4	1.4516453837912688E-5	0.0	1.5968099221703956E-4	2.1774680756869033E-5
54	5.189632247053785E-4	1.4516453837912688E-5	0.0	1.5968099221703956E-4	2.1774680756869033E-5
55	5.262214516243349E-4	1.4516453837912688E-5	0.0	1.705683325954741E-4	2.1774680756869033E-5
56	5.47996132381204E-4	1.4516453837912688E-5	0.0	1.8508478643338677E-4	2.1774680756869033E-5
57	5.588834727596384E-4	1.4516453837912688E-5	0.0	1.9234301335234311E-4	2.1774680756869033E-5
58	5.988037208138983E-4	1.4516453837912688E-5	0.0	1.9234301335234311E-4	2.1774680756869033E-5
59	6.459821957871146E-4	1.4516453837912688E-5	0.0	1.9234301335234311E-4	2.1774680756869033E-5
60	6.859024438413745E-4	1.4516453837912688E-5	0.0	1.9234301335234311E-4	2.1774680756869033E-5
61	7.076771245982435E-4	1.4516453837912688E-5	0.0	1.9960124027129945E-4	2.1774680756869033E-5
62	7.18564464976678E-4	1.4516453837912688E-5	0.0	2.0323035373077762E-4	2.1774680756869033E-5
63	7.221935784361562E-4	1.4516453837912688E-5	0.0	2.1048858064973396E-4	2.1774680756869033E-5
64	7.221935784361562E-4	1.4516453837912688E-5	0.0	2.177468075686903E-4	2.1774680756869033E-5
65	7.258226918956344E-4	1.814556729739086E-5	0.0	2.213759210281685E-4	2.5403794216347203E-5
>>END_MODULE
