##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543824_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27554939
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.556884303028216	32.0	32.0	32.0	32.0	32.0
2	31.689054836956817	32.0	32.0	32.0	32.0	32.0
3	35.82321731868105	37.0	37.0	37.0	32.0	37.0
4	36.413189773346986	37.0	37.0	37.0	37.0	37.0
5	36.57503778179295	37.0	37.0	37.0	37.0	37.0
6	40.09951108946385	41.0	41.0	41.0	37.0	41.0
7	40.15451298948621	41.0	41.0	41.0	37.0	41.0
8	40.075080224274856	41.0	41.0	41.0	37.0	41.0
9	40.01608622686481	41.0	41.0	41.0	37.0	41.0
10-11	39.79999082923029	41.0	41.0	41.0	37.0	41.0
12-13	39.68279007984739	41.0	41.0	41.0	37.0	41.0
14-15	39.65903969157762	41.0	41.0	41.0	37.0	41.0
16-17	39.66525774199681	41.0	41.0	41.0	37.0	41.0
18-19	39.64153150547712	41.0	41.0	41.0	37.0	41.0
20-21	39.62631742715888	41.0	41.0	41.0	37.0	41.0
22-23	39.61914600863388	41.0	41.0	41.0	37.0	41.0
24-25	39.597904626099876	41.0	41.0	41.0	37.0	41.0
26-27	39.478013088688016	41.0	41.0	41.0	37.0	41.0
28-29	39.49385240881861	41.0	41.0	41.0	37.0	41.0
30-31	39.54039633330344	41.0	41.0	41.0	37.0	41.0
32-33	39.60217596562271	41.0	41.0	41.0	37.0	41.0
34-35	39.61352750590375	41.0	41.0	41.0	37.0	41.0
36-37	39.74417353115389	41.0	41.0	41.0	37.0	41.0
38-39	39.83018626404955	41.0	41.0	41.0	37.0	41.0
40-41	39.90732384895675	41.0	41.0	41.0	37.0	41.0
42-43	39.91915840484455	41.0	41.0	41.0	37.0	41.0
44-45	39.92510887246506	41.0	41.0	41.0	37.0	41.0
46-47	39.84692929289223	41.0	41.0	41.0	37.0	41.0
48-49	39.91668042864369	41.0	41.0	41.0	37.0	41.0
50-51	39.89737848073347	41.0	41.0	41.0	37.0	41.0
52-53	39.84635389780752	41.0	41.0	41.0	37.0	41.0
54-55	39.88949988044595	41.0	41.0	41.0	37.0	41.0
56-57	39.888190813804485	41.0	41.0	41.0	37.0	41.0
58-59	39.8188447251312	41.0	41.0	41.0	37.0	41.0
60-61	39.85247983871878	41.0	41.0	41.0	37.0	41.0
62-63	39.83181175585008	41.0	41.0	41.0	37.0	41.0
64-65	39.77301748378714	41.0	41.0	41.0	37.0	41.0
66-67	39.77418600406543	41.0	41.0	41.0	37.0	41.0
68-69	39.762605440218906	41.0	41.0	41.0	37.0	41.0
70-71	39.744632187177174	41.0	41.0	41.0	37.0	41.0
72-73	39.69392139066055	41.0	41.0	41.0	37.0	41.0
74-75	39.67627884556628	41.0	41.0	41.0	37.0	41.0
76	39.17628549717179	41.0	41.0	41.0	37.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10886.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	0.0
15	2.0
16	9.0
17	13.0
18	64.0
19	176.0
20	401.0
21	996.0
22	2232.0
23	4324.0
24	7113.0
25	12862.0
26	21543.0
27	34678.0
28	54072.0
29	79802.0
30	113063.0
31	158264.0
32	214645.0
33	288334.0
34	386758.0
35	519865.0
36	717667.0
37	1052965.0
38	1815648.0
39	5295108.0
40	1.6763448E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81445381394085	10.251177213626788	11.9366215456646	35.99774742676776
2	23.10253648275665	14.848372161079443	33.41737940239721	28.631711953766693
3	22.51120414268735	19.43560738864393	27.18085098079066	30.872337487878056
4	27.344324804770615	26.448095923456428	20.51662263687842	25.69095663489454
5	26.70124472966996	29.65313419924076	23.963158217855593	19.68246285323369
6	22.117944260187812	32.09336505129917	26.36975420900028	19.418936479512734
7	18.958843856421566	22.15006266506966	41.35021813964706	17.540875338861714
8	21.530214762175273	21.99801169060285	32.9528064602665	23.51896708695537
9	21.70551661369516	21.6161688332505	34.36214343618929	22.316171116865043
10-11	24.54084553206458	29.41463444032728	25.525288525984173	20.519231501623963
12-13	24.231051451130288	23.49985802686275	29.576836548057045	22.692253973949917
14-15	23.348579605410265	25.445520786903835	29.710892400853457	21.495007206832444
16-17	23.644349506213533	25.752687764093444	28.98596467011625	21.61699805957677
18-19	23.709493043134415	26.047331929170404	28.703270374241814	21.53990465345337
20-21	23.356150480552042	25.861909346595386	29.129244641610637	21.652695531241935
22-23	23.813911422892772	25.57519034868967	28.682372657658302	21.928525570759255
24-25	23.713877505712063	25.695558066515588	28.61049585127339	21.980068576498958
26-27	23.44197812326426	25.991189795201635	28.62859065679678	21.938241424737328
28-29	23.82075698205331	25.7709617277082	28.52605731244402	21.882223977794464
30-31	23.514569033639052	25.922499288659434	28.409559770916477	22.15337190678504
32-33	23.442443336806477	25.645776612466932	28.717661605802082	22.19411844492451
34-35	23.632818886465223	25.757039857139535	28.496624617031657	22.113516639363585
36-37	23.947028416383226	25.584938115093564	28.276479850004726	22.191553618518483
38-39	23.55799968731609	25.965992628867262	28.54659544058924	21.929412243227407
40-41	23.89117532295027	25.421434723536855	28.580849291252452	22.106540662260414
42-43	23.539272036740076	25.287108590281175	28.9056646498437	22.26795472313505
44-45	23.700020098903316	25.342203128617268	28.928198052940314	22.029578719539103
46-47	23.66901594242503	25.60493478349889	28.60287836331088	22.1231709107652
48-49	23.68312831828594	25.543585270431834	28.433978724738836	22.339307686543393
50-51	23.461389064013968	25.667139916343203	28.683163327916734	22.1883076917261
52-53	23.677953454589456	25.479511595484034	28.46128878044893	22.381246169477585
54-55	23.519471508906022	25.729849348416394	28.27540797071821	22.47527117195938
56-57	23.80892612296256	25.427358583144876	28.595293496684544	22.168421797208023
58-59	23.753079309186358	25.328418087345156	28.515907387367985	22.402595216100497
60-61	23.44711287178504	25.400901761045013	28.58394547171534	22.568039895454604
62-63	23.345842517301563	25.61142441976047	28.796668956911248	22.246064106026722
64-65	23.75850843099439	25.51312248488297	28.425258598700005	22.303110485422636
66-67	23.88367104048115	25.37481906508901	28.301520463691215	22.439989430738628
68-69	23.615683971252917	25.72403317012691	28.434877451427642	22.22540540719253
70-71	23.88793454845097	25.53046594300469	28.309558041716826	22.27204146682751
72-73	23.76212805748083	25.214360762834875	28.440660172909006	22.58285100677529
74-75	23.9711675790493	22.123427676171456	29.997068767493662	23.908335977285578
76	24.95985285696949	0.0	41.32277361063832	33.717373532392195
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	316517.0
1	224176.0
2	83583.5
3	32218.5
4	29105.5
5	32647.5
6	45532.0
7	65117.0
8	75368.0
9	74866.0
10	85653.0
11	84522.5
12	72098.0
13	57796.0
14	29106.0
15	12684.5
16	10375.5
17	10461.0
18	11969.0
19	16362.0
20	21988.0
21	26621.0
22	34737.0
23	47353.0
24	62459.5
25	77000.0
26	106553.0
27	152123.5
28	196742.5
29	237622.5
30	302931.5
31	381280.5
32	455443.0
33	516082.0
34	591772.0
35	713220.0
36	819286.5
37	867264.0
38	870671.5
39	905590.0
40	972386.5
41	1017614.5
42	1035568.0
43	1074768.0
44	1160357.0
45	1257450.0
46	1305389.5
47	1339066.0
48	1369509.0
49	1369078.5
50	1364727.5
51	1359527.5
52	1363993.0
53	1336639.0
54	1304442.5
55	1248044.0
56	1145406.5
57	1031849.5
58	948784.5
59	861331.5
60	742078.5
61	614454.0
62	518740.0
63	446019.5
64	355583.5
65	281853.5
66	209953.5
67	167755.5
68	137489.0
69	101747.5
70	77688.0
71	64470.5
72	56375.5
73	46690.0
74	37312.0
75	30288.5
76	26582.0
77	21335.0
78	17775.0
79	17505.0
80	15157.0
81	13170.5
82	11801.0
83	9627.5
84	8226.0
85	6843.0
86	6336.5
87	6058.0
88	4078.0
89	1746.0
90	732.5
91	259.0
92	187.0
93	137.0
94	74.0
95	59.0
96	57.0
97	38.0
98	13.5
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8402159772518458
2	0.09575778774179106
3	0.03950652911987938
4	0.0471022635905672
5	0.03950652911987938
6	0.039658951885177464
7	0.03950652911987938
8	0.039687984792853286
9	0.03950652911987938
10-11	0.03950652911987938
12-13	0.039905731600421976
14-15	0.039695243019772244
16-17	0.039731534154367025
18-19	0.03951923101698755
20-21	0.039513787346798335
22-23	0.039733348711096766
24-25	0.04081482452202126
26-27	0.049288804449902794
28-29	0.05103077891045232
30-31	0.04946663100941722
32-33	0.052812673619056096
34-35	0.056684937680319306
36-37	4.5391724133648565E-4
38-39	0.0014272128008422952
40-41	3.6864001543421293E-4
42-43	5.448549177578733E-6
44-45	0.0
46-47	1.4533773091394942E-5
48-49	6.159825895337509E-4
50-51	4.689097394188609E-4
52-53	1.9270744838649143E-4
54-55	1.3275765250571702E-4
56-57	0.0016646406867619784
58-59	0.0019493008152827807
60-61	7.284073210034612E-6
62-63	0.00263845540738863
64-65	0.0
66-67	0.0011405678940466808
68-69	0.0011799808108817172
70-71	1.8285497283881375E-6
72-73	7.3443283314295614E-6
74-75	6.583056909864785E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	16497.0
36	765.0
37	946.0
38	1086.0
39	1273.0
40	1472.0
41	1706.0
42	1859.0
43	1970.0
44	1975.0
45	2147.0
46	2280.0
47	2501.0
48	2901.0
49	3190.0
50	3492.0
51	3953.0
52	4194.0
53	4600.0
54	4850.0
55	5053.0
56	5641.0
57	5948.0
58	6503.0
59	7125.0
60	7694.0
61	8473.0
62	9096.0
63	9705.0
64	10455.0
65	10889.0
66	12136.0
67	12531.0
68	13413.0
69	14389.0
70	16313.0
71	46550.0
72	114940.0
73	506342.0
74	1992244.0
75	6415514.0
76	1.8260328E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.441658229809985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.93372709687281	17.962803941726925
2	14.37670112200291	7.602876350802534
3	5.590783325402377	4.434887457816306
4	2.9720169016041704	3.1434022066174516
5	1.7896601415114781	2.366079090467994
6	1.2444411420276935	1.9743052418766418
7	0.872672890251369	1.6152442817313022
8	0.6602817420543794	1.3967155327028362
9	0.5064170763420415	1.205145652893827
>10	3.3717875118821654	17.180939790154923
>50	0.35375180077696344	6.513258953035295
>100	0.2743175256712408	14.618708332352396
>500	0.032635360668088614	5.9465768835833535
>1k	0.01989145162976911	9.422409880344867
>5k	6.535080731922522E-4	1.1297941506592903
>10k+	2.614032292767272E-4	3.486852253234232
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	281861	1.022905548801977	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100898	0.36617028983442856	No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89547	0.32497622295589185	No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70208	0.25479279776304353	No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65245	0.23678150766365333	No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59794	0.2169992101960378	No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49466	0.17951772638654726	No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29456	0.10689916606238903	No Hit
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28318	0.10276923494550287	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.258226918956344E-6	0.0
2	3.629113459478172E-6	7.258226918956344E-6	0.0	7.258226918956344E-6	1.814556729739086E-5
3	3.629113459478172E-6	7.258226918956344E-6	0.0	1.0887340378434517E-5	2.5403794216347203E-5
4	2.1774680756869033E-5	7.258226918956344E-6	0.0	1.4516453837912688E-5	2.9032907675825376E-5
5	2.5403794216347203E-5	7.258226918956344E-6	0.0	1.814556729739086E-5	2.9032907675825376E-5
6	2.5403794216347203E-5	7.258226918956344E-6	0.0	1.814556729739086E-5	3.266202113530355E-5
7	2.5403794216347203E-5	7.258226918956344E-6	0.0	1.814556729739086E-5	3.629113459478172E-5
8	2.5403794216347203E-5	7.258226918956344E-6	0.0	2.1774680756869033E-5	3.992024805425989E-5
9	2.5403794216347203E-5	7.258226918956344E-6	0.0	2.1774680756869033E-5	5.0807588432694406E-5
10	2.5403794216347203E-5	7.258226918956344E-6	0.0	2.5403794216347203E-5	5.0807588432694406E-5
11	3.266202113530355E-5	7.258226918956344E-6	0.0	3.266202113530355E-5	5.0807588432694406E-5
12	3.266202113530355E-5	7.258226918956344E-6	0.0	3.266202113530355E-5	6.169492881112893E-5
13	3.266202113530355E-5	7.258226918956344E-6	0.0	3.266202113530355E-5	8.346960956799795E-5
14	3.629113459478172E-5	7.258226918956344E-6	0.0	3.629113459478172E-5	8.346960956799795E-5
15	3.629113459478172E-5	7.258226918956344E-6	0.0	3.629113459478172E-5	8.346960956799795E-5
16	3.992024805425989E-5	7.258226918956344E-6	0.0	3.992024805425989E-5	8.346960956799795E-5
17	4.3549361513738066E-5	7.258226918956344E-6	0.0	3.992024805425989E-5	1.0161517686538881E-4
18	5.0807588432694406E-5	7.258226918956344E-6	0.0	3.992024805425989E-5	1.0887340378434515E-4
19	5.806581535165075E-5	7.258226918956344E-6	3.629113459478172E-6	3.992024805425989E-5	1.0887340378434515E-4
20	6.169492881112893E-5	7.258226918956344E-6	3.629113459478172E-6	4.3549361513738066E-5	1.2701897108173603E-4
21	6.895315573008527E-5	7.258226918956344E-6	3.629113459478172E-6	4.3549361513738066E-5	1.306480845412142E-4
22	7.258226918956344E-5	1.4516453837912688E-5	3.629113459478172E-6	4.7178474973216236E-5	1.306480845412142E-4
23	7.621138264904161E-5	1.4516453837912688E-5	3.629113459478172E-6	5.4436701892172576E-5	1.306480845412142E-4
24	7.984049610851978E-5	1.4516453837912688E-5	3.629113459478172E-6	5.4436701892172576E-5	1.306480845412142E-4
25	9.07278364869543E-5	1.4516453837912688E-5	3.629113459478172E-6	6.53240422706071E-5	1.3427719800069237E-4
26	1.1250251724382334E-4	1.4516453837912688E-5	3.629113459478172E-6	6.53240422706071E-5	1.3427719800069237E-4
27	1.1250251724382334E-4	1.4516453837912688E-5	3.629113459478172E-6	6.53240422706071E-5	1.3427719800069237E-4
28	1.161316307033015E-4	1.4516453837912688E-5	3.629113459478172E-6	6.53240422706071E-5	1.3427719800069237E-4
29	1.161316307033015E-4	1.814556729739086E-5	3.629113459478172E-6	6.53240422706071E-5	1.415354249196487E-4
30	1.2338985762225786E-4	1.814556729739086E-5	3.629113459478172E-6	6.53240422706071E-5	1.415354249196487E-4
31	1.3427719800069237E-4	1.814556729739086E-5	3.629113459478172E-6	6.53240422706071E-5	1.415354249196487E-4
32	1.3427719800069237E-4	1.814556729739086E-5	3.629113459478172E-6	7.258226918956344E-5	1.4879365183860505E-4
33	1.814556729739086E-4	1.814556729739086E-5	3.629113459478172E-6	7.258226918956344E-5	1.4879365183860505E-4
34	2.0323035373077762E-4	1.814556729739086E-5	3.629113459478172E-6	7.984049610851978E-5	1.4879365183860505E-4
35	2.068594671902558E-4	1.814556729739086E-5	3.629113459478172E-6	7.984049610851978E-5	1.4879365183860505E-4
36	2.068594671902558E-4	1.814556729739086E-5	3.629113459478172E-6	7.984049610851978E-5	1.560518787575614E-4
37	2.1048858064973396E-4	1.814556729739086E-5	3.629113459478172E-6	8.709872302747613E-5	1.560518787575614E-4
38	2.1411769410921213E-4	1.814556729739086E-5	3.629113459478172E-6	9.435694994643247E-5	1.9234301335234311E-4
39	2.213759210281685E-4	1.814556729739086E-5	3.629113459478172E-6	9.798606340591064E-5	1.9597212681182128E-4
40	2.2500503448764667E-4	1.814556729739086E-5	3.629113459478172E-6	1.0524429032486698E-4	2.0323035373077762E-4
41	2.2500503448764667E-4	1.814556729739086E-5	3.629113459478172E-6	1.1250251724382334E-4	2.32263261406603E-4
42	2.32263261406603E-4	1.814556729739086E-5	3.629113459478172E-6	1.161316307033015E-4	2.3589237486608118E-4
43	2.3589237486608118E-4	1.814556729739086E-5	7.258226918956344E-6	1.161316307033015E-4	2.467797152445157E-4
44	2.5040882870399386E-4	2.1774680756869033E-5	1.0887340378434517E-5	1.161316307033015E-4	2.467797152445157E-4
45	2.5403794216347206E-4	2.1774680756869033E-5	1.0887340378434517E-5	1.161316307033015E-4	2.5040882870399386E-4
46	3.2662021135303546E-4	2.1774680756869033E-5	1.4516453837912688E-5	1.1976074416277968E-4	2.5403794216347206E-4
47	3.3024932481251365E-4	2.1774680756869033E-5	1.4516453837912688E-5	1.1976074416277968E-4	2.6855439600138474E-4
48	3.411366651909482E-4	2.1774680756869033E-5	1.4516453837912688E-5	1.1976074416277968E-4	2.721835094608629E-4
49	3.411366651909482E-4	2.1774680756869033E-5	1.4516453837912688E-5	1.2701897108173603E-4	2.794417363798192E-4
50	3.4839489210990453E-4	2.1774680756869033E-5	1.4516453837912688E-5	1.2701897108173603E-4	2.975873036772101E-4
51	3.8105691324520803E-4	2.1774680756869033E-5	1.4516453837912688E-5	1.2701897108173603E-4	2.975873036772101E-4
52	3.8468602670468623E-4	2.1774680756869033E-5	1.814556729739086E-5	1.3427719800069237E-4	3.0484553059616644E-4
53	3.883151401641644E-4	2.1774680756869033E-5	1.814556729739086E-5	1.3427719800069237E-4	3.0847464405564463E-4
54	3.883151401641644E-4	2.9032907675825376E-5	2.1774680756869033E-5	1.3790631146017054E-4	3.0847464405564463E-4
55	3.9557336708312077E-4	2.9032907675825376E-5	2.5403794216347203E-5	1.4879365183860505E-4	3.0847464405564463E-4
56	3.9557336708312077E-4	2.9032907675825376E-5	2.5403794216347203E-5	1.4879365183860505E-4	3.0847464405564463E-4
57	3.992024805425989E-4	3.266202113530355E-5	2.5403794216347203E-5	1.4879365183860505E-4	3.0847464405564463E-4
58	4.354936151373806E-4	3.629113459478172E-5	2.5403794216347203E-5	1.5968099221703956E-4	3.0847464405564463E-4
59	4.5001006897529334E-4	3.629113459478172E-5	2.5403794216347203E-5	1.5968099221703956E-4	3.121037575151228E-4
60	4.536391824347715E-4	3.629113459478172E-5	2.5403794216347203E-5	1.5968099221703956E-4	3.15732870974601E-4
61	4.608974093537278E-4	3.629113459478172E-5	2.5403794216347203E-5	1.5968099221703956E-4	3.15732870974601E-4
62	4.64526522813206E-4	3.629113459478172E-5	2.5403794216347203E-5	1.669392191359959E-4	3.193619844340791E-4
63	4.64526522813206E-4	3.629113459478172E-5	2.5403794216347203E-5	1.669392191359959E-4	3.2662021135303546E-4
64	4.64526522813206E-4	3.629113459478172E-5	2.5403794216347203E-5	1.669392191359959E-4	3.3024932481251365E-4
65	4.6815563627268416E-4	3.992024805425989E-5	2.9032907675825376E-5	1.705683325954741E-4	3.4839489210990453E-4
>>END_MODULE
