##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543823_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24606185
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.49618297188288	32.0	32.0	32.0	32.0	32.0
2	30.667258455546847	32.0	32.0	32.0	32.0	32.0
3	32.73626955986879	37.0	32.0	37.0	22.0	37.0
4	34.56725453376865	37.0	37.0	37.0	27.0	37.0
5	35.17746131714445	37.0	37.0	37.0	32.0	37.0
6	38.345086570713825	41.0	41.0	41.0	32.0	41.0
7	38.129211984710345	41.0	41.0	41.0	32.0	41.0
8	38.11878253374101	41.0	41.0	41.0	32.0	41.0
9	38.29780874198906	41.0	41.0	41.0	32.0	41.0
10-11	38.17468990012064	41.0	41.0	41.0	29.5	41.0
12-13	38.14785083506443	41.0	41.0	41.0	32.0	41.0
14-15	38.324646547199414	41.0	41.0	41.0	32.0	41.0
16-17	38.26735906033382	41.0	41.0	41.0	32.0	41.0
18-19	38.36137111462017	41.0	41.0	41.0	32.0	41.0
20-21	38.431451726466335	41.0	41.0	41.0	32.0	41.0
22-23	38.29716800877503	41.0	41.0	41.0	32.0	41.0
24-25	38.31196132191967	41.0	41.0	41.0	32.0	41.0
26-27	38.453087811052384	41.0	41.0	41.0	32.0	41.0
28-29	38.26422730707747	41.0	39.0	41.0	32.0	41.0
30-31	38.357572760669726	41.0	41.0	41.0	32.0	41.0
32-33	38.52991766094581	41.0	41.0	41.0	32.0	41.0
34-35	38.4852387316441	41.0	41.0	41.0	32.0	41.0
36-37	38.356156619580375	41.0	41.0	41.0	32.0	41.0
38-39	38.3249003921225	41.0	41.0	41.0	32.0	41.0
40-41	38.363916819820076	41.0	41.0	41.0	32.0	41.0
42-43	38.339049676710275	41.0	41.0	41.0	32.0	41.0
44-45	38.29941759510224	41.0	39.0	41.0	32.0	41.0
46-47	38.314551124484616	41.0	37.0	41.0	32.0	41.0
48-49	38.29503337930217	41.0	37.0	41.0	32.0	41.0
50-51	38.23970462743978	41.0	37.0	41.0	32.0	41.0
52-53	38.25965727629171	41.0	37.0	41.0	32.0	41.0
54-55	38.26747911561055	41.0	37.0	41.0	32.0	41.0
56-57	38.264271269649164	41.0	37.0	41.0	32.0	41.0
58-59	38.27671179517567	41.0	37.0	41.0	32.0	41.0
60-61	38.234695069897704	41.0	37.0	41.0	32.0	41.0
62-63	38.2595750672251	41.0	37.0	41.0	32.0	41.0
64-65	38.244814257135744	41.0	37.0	41.0	32.0	41.0
66-67	38.15949974088211	41.0	37.0	41.0	32.0	41.0
68-69	38.16564560014305	41.0	37.0	41.0	32.0	41.0
70-71	37.992198166298294	41.0	37.0	41.0	32.0	41.0
72-73	38.09310424455683	41.0	37.0	41.0	32.0	41.0
74-75	38.11739594946452	41.0	37.0	41.0	32.0	41.0
76	37.26776193949305	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29089.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	106.0
13	413.0
14	1700.0
15	5642.0
16	15217.0
17	32644.0
18	51929.0
19	69393.0
20	82395.0
21	93269.0
22	104135.0
23	116986.0
24	131911.0
25	147149.0
26	165831.0
27	187085.0
28	213041.0
29	244717.0
30	282785.0
31	328069.0
32	382831.0
33	451603.0
34	546793.0
35	685981.0
36	910446.0
37	1327892.0
38	2327128.0
39	5982639.0
40	9687366.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.43478306680833	18.943071207355924	13.710871482311573	32.911274243524176
2	21.29138206022435	27.013846291436046	28.070611258140936	23.624160390198668
3	20.60301998250729	27.901750475320597	26.826692624710418	24.66853691746169
4	24.16861654032668	32.24254608186271	18.88406898764632	24.704768390164286
5	23.58405325682867	34.07490763313536	19.735646159531395	22.605392950504577
6	19.252941985454658	36.16441672559725	20.58979270812396	23.992848580824138
7	19.36326251871869	21.493482777988362	32.78405907042656	26.35919563286639
8	20.248878609932035	23.524859378662015	25.849595388140933	30.37666662326502
9	21.161514748777808	23.34179168068242	27.030321757225835	28.46637181331394
10-11	22.8576396070147	30.391470461753535	22.287177076326838	24.463712854904927
12-13	22.620458559496864	24.842899820554297	27.622942073349055	24.913699546599783
14-15	21.7745571852557	27.494818118839813	28.442263578172454	22.28836111773203
16-17	22.044526362525787	27.5036806986215	27.895673474263756	22.55611946458896
18-19	21.922941060015482	27.77498683244129	27.94262258544997	22.35944952209325
20-21	21.955103504948326	27.72695228458583	28.030057294693744	22.287886915772095
22-23	22.08280522245433	27.415891495412463	27.969014592654425	22.532288689478776
24-25	22.002788425059464	27.28788481660501	28.175585205791055	22.53374155254447
26-27	21.81135949739507	27.602064771313444	28.08130326960373	22.505272461687756
28-29	22.351750011698616	26.959410877884725	28.067569189702773	22.62126992071388
30-31	22.04535102749725	27.188619787907843	28.341970940251837	22.424058244343065
32-33	21.88638953502403	27.429417821533324	28.368832093116065	22.31536055032658
34-35	22.141106962044468	27.308341566675253	27.971772120088715	22.57877935119156
36-37	21.84644452462837	27.126904088423743	28.42481322131191	22.601838165635975
38-39	21.63342373611736	27.3724187157027	28.617165502795867	22.37699204538407
40-41	22.102449759526294	27.072395050035748	28.232370026513347	22.59278516392461
42-43	21.747463729639964	27.123542116876916	28.442100980434954	22.68689317304817
44-45	21.832661114240334	26.98783908958285	28.483943418545387	22.69555637763143
46-47	22.02719172644369	26.88942868868954	28.287229425983558	22.796150158883208
48-49	21.854086499787748	26.92369084563671	28.52937705327223	22.69284560130331
50-51	21.68059472009679	27.056451228336687	28.561873562636542	22.701080488929986
52-53	21.900196149145764	26.944787926464464	28.26713883204507	22.887877092344706
54-55	21.833573851821235	26.7844447510626	28.637927027394426	22.744054369721738
56-57	21.68157151598607	27.162446109107837	28.399152726512426	22.756829648393666
58-59	21.802975027673295	27.144727203182008	28.402459940247603	22.64983782889709
60-61	21.772182891112152	26.91225367771379	28.64062319794261	22.674940233231446
62-63	21.60222419401963	27.28169983736453	28.502670890147968	22.613405078467867
64-65	21.976122402581282	27.048426611742737	28.399194103315146	22.57625688236084
66-67	21.767480372583034	27.039346084239597	28.63378138818094	22.55939215499643
68-69	21.596461947575705	27.184810159691665	28.822086769633692	22.39664112309894
70-71	21.894102062533292	27.203104032444553	28.480433093162922	22.42236081185923
72-73	21.648950434017145	26.970131077058774	28.654261913431444	22.726656575492633
74-75	21.4471304864422	24.009713128013455	30.455814883932796	24.087341501611544
76	22.533554478070624	0.0	42.75345368463099	34.71299183729839
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	184013.0
1	107785.0
2	29319.5
3	27132.5
4	27183.5
5	24622.0
6	26695.5
7	37958.5
8	44594.5
9	48525.5
10	63023.5
11	158644.0
12	243704.5
13	313548.0
14	284567.0
15	146324.0
16	74170.5
17	42518.5
18	38664.5
19	35599.0
20	41280.0
21	48290.5
22	58718.0
23	76059.5
24	98878.5
25	120339.5
26	142532.0
27	176844.5
28	218067.5
29	245806.5
30	292159.0
31	368980.0
32	431050.0
33	465383.5
34	536267.0
35	630375.0
36	685464.5
37	713455.5
38	736440.0
39	790131.5
40	847343.5
41	878972.0
42	897532.5
43	950654.0
44	1038605.5
45	1102863.5
46	1134963.5
47	1175006.5
48	1205025.0
49	1208803.5
50	1203851.5
51	1189907.5
52	1179321.0
53	1150477.5
54	1112430.5
55	1044209.5
56	935918.0
57	818999.0
58	742269.5
59	674077.5
60	579347.5
61	486212.0
62	416153.5
63	352539.5
64	279921.0
65	219731.5
66	165845.0
67	139846.0
68	121054.5
69	94601.0
70	72548.5
71	60060.5
72	54423.0
73	50703.5
74	41744.0
75	31950.0
76	25128.5
77	18771.5
78	16184.0
79	15157.0
80	14560.5
81	13812.5
82	12596.5
83	11624.0
84	9952.5
85	8083.5
86	7530.5
87	7222.5
88	6198.0
89	3758.0
90	1699.5
91	955.0
92	775.0
93	708.5
94	560.5
95	438.0
96	398.0
97	350.5
98	273.5
99	217.0
100	190.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.2594516378707223
2	0.2551797444423018
3	0.1182182447218047
4	0.11939681019223419
5	0.12082734483220377
6	0.1188522316645185
7	0.1184620858536177
8	0.1200714373235835
9	0.12059569575698142
10-11	0.11901885643792404
12-13	0.11831374916509814
14-15	0.11868154287224941
16-17	0.11919360924905668
18-19	0.12064243197391225
20-21	0.11943745038086968
22-23	0.11865512674963632
24-25	0.1246170424224641
26-27	0.12153042009559792
28-29	0.12450121788485292
30-31	0.13027618868995744
32-33	0.13822540958706114
34-35	0.14576213256951454
36-37	0.0027860085551423847
38-39	0.004050182452578419
40-41	0.001111402350998653
42-43	0.0021356367101577176
44-45	0.001474257559108005
46-47	1.6089825380994846E-4
48-49	0.004023440339326568
50-51	0.0038045659478305772
52-53	0.0015390964961316492
54-55	0.009748283216178887
56-57	0.004082720112581058
58-59	0.004160442079838067
60-61	0.0010642430414080626
62-63	2.800492314283921E-4
64-65	6.68977906912563E-4
66-67	0.005053733804196074
68-69	0.0036465956457147233
70-71	0.003804721967787426
72-73	0.0013994140927305776
74-75	2.6529425199660784E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	36447.0
36	1077.0
37	1178.0
38	1377.0
39	1576.0
40	1937.0
41	2005.0
42	2335.0
43	2354.0
44	2333.0
45	2529.0
46	2722.0
47	2959.0
48	3368.0
49	3645.0
50	4071.0
51	4504.0
52	4782.0
53	5122.0
54	5453.0
55	5674.0
56	6032.0
57	6590.0
58	7051.0
59	7532.0
60	8081.0
61	8848.0
62	9236.0
63	9872.0
64	10452.0
65	11316.0
66	12274.0
67	12455.0
68	12768.0
69	13590.0
70	16358.0
71	37507.0
72	117244.0
73	544023.0
74	1705283.0
75	6087502.0
76	1.5864723E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.602115766101306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.0107375764907	23.704980125929044
2	11.597731199411832	7.330256879758753
3	4.289056857691302	4.0662981403245375
4	2.2478036555761753	2.84141405371936
5	1.3485954947375964	2.130923547317009
6	0.889492598495725	1.6865908842451305
7	0.6503909145854248	1.438761028316437
8	0.49084428386685375	1.2409374305511522
9	0.3890917831383096	1.1066511216938082
>10	2.542086768855912	15.59753156174271
>50	0.2756887310954768	6.083583182535753
>100	0.22743355863439665	14.677107648116266
>500	0.027635626488194044	5.976267709697913
>1k	0.013012729588838179	6.832398412129197
>5k	1.3731770455514502E-4	0.2896289109988363
>10k+	2.609036386547698E-4	4.996669362924329
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	356125	1.4472987177817285	No Hit
CCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	155784	0.6331091146392664	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	121713	0.4946439279392559	No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	90973	0.36971598807373024	No Hit
CCCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82453	0.3350905473562846	No Hit
CCCCCTCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40645	0.165182046708988	No Hit
CCCCTCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40581	0.16492194950172082	No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32756	0.1331210018944424	No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32288	0.131219041066301	No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30099	0.12232290377399016	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	29089	0.1182182447218047	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28123	0.11429240249961545	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	8.128037727099914E-6	0.0	0.0	0.0
2	0.0	8.128037727099914E-6	0.0	0.0	0.0
3	0.0	8.128037727099914E-6	0.0	0.0	0.0
4	0.0	8.128037727099914E-6	0.0	0.0	4.064018863549957E-6
5	0.0	8.128037727099914E-6	0.0	4.064018863549957E-6	4.064018863549957E-6
6	0.0	8.128037727099914E-6	0.0	4.064018863549957E-6	4.064018863549957E-6
7	0.0	8.128037727099914E-6	0.0	4.470420749904953E-5	8.128037727099914E-6
8	0.0	8.128037727099914E-6	0.0	4.470420749904953E-5	8.128037727099914E-6
9	0.0	8.128037727099914E-6	0.0	5.2832245226149444E-5	8.128037727099914E-6
10	8.128037727099914E-6	8.128037727099914E-6	0.0	5.2832245226149444E-5	8.128037727099914E-6
11	2.0320094317749786E-5	8.128037727099914E-6	0.0	5.2832245226149444E-5	8.128037727099914E-6
12	2.4384113181299743E-5	8.128037727099914E-6	0.0	5.2832245226149444E-5	8.128037727099914E-6
13	2.84481320448497E-5	8.128037727099914E-6	0.0	5.2832245226149444E-5	8.128037727099914E-6
14	3.6576169771949615E-5	8.128037727099914E-6	0.0	5.2832245226149444E-5	8.128037727099914E-6
15	5.2832245226149444E-5	8.128037727099914E-6	0.0	5.2832245226149444E-5	8.128037727099914E-6
16	6.502430181679932E-5	8.128037727099914E-6	0.0	5.2832245226149444E-5	8.128037727099914E-6
17	6.908832068034927E-5	8.128037727099914E-6	0.0	5.68962640896994E-5	8.128037727099914E-6
18	6.908832068034927E-5	8.128037727099914E-6	0.0	6.096028295324936E-5	8.128037727099914E-6
19	7.315233954389923E-5	8.128037727099914E-6	0.0	6.096028295324936E-5	8.128037727099914E-6
20	7.315233954389923E-5	8.128037727099914E-6	0.0	6.908832068034927E-5	1.2192056590649872E-5
21	7.721635840744919E-5	8.128037727099914E-6	0.0	6.908832068034927E-5	1.625607545419983E-5
22	8.53443961345491E-5	8.128037727099914E-6	0.0	6.908832068034927E-5	1.625607545419983E-5
23	8.940841499809906E-5	8.128037727099914E-6	0.0	6.908832068034927E-5	1.625607545419983E-5
24	8.940841499809906E-5	8.128037727099914E-6	0.0	6.908832068034927E-5	1.625607545419983E-5
25	8.940841499809906E-5	8.128037727099914E-6	0.0	6.908832068034927E-5	1.625607545419983E-5
26	1.2192056590649872E-4	8.128037727099914E-6	0.0	6.908832068034927E-5	1.625607545419983E-5
27	1.3004860363359863E-4	8.128037727099914E-6	0.0	6.908832068034927E-5	2.0320094317749786E-5
28	1.422406602242485E-4	1.2192056590649872E-5	0.0	6.908832068034927E-5	2.0320094317749786E-5
29	1.5443271681489837E-4	1.2192056590649872E-5	0.0	6.908832068034927E-5	2.0320094317749786E-5
30	1.625607545419983E-4	1.2192056590649872E-5	0.0	6.908832068034927E-5	2.0320094317749786E-5
31	1.7475281113264816E-4	1.2192056590649872E-5	0.0	6.908832068034927E-5	2.0320094317749786E-5
32	1.7881682999619812E-4	1.625607545419983E-5	0.0	6.908832068034927E-5	2.0320094317749786E-5
33	1.91008886586848E-4	1.625607545419983E-5	0.0	6.908832068034927E-5	2.0320094317749786E-5
34	1.9507290545039795E-4	1.625607545419983E-5	0.0	7.315233954389923E-5	2.0320094317749786E-5
35	2.1132898090459777E-4	1.625607545419983E-5	0.0	7.721635840744919E-5	2.0320094317749786E-5
36	2.3164907522234756E-4	1.625607545419983E-5	0.0	7.721635840744919E-5	2.0320094317749786E-5
37	2.5196916954009735E-4	1.625607545419983E-5	0.0	7.721635840744919E-5	2.0320094317749786E-5
38	2.6009720726719726E-4	2.4384113181299743E-5	0.0	8.128037727099914E-5	2.0320094317749786E-5
39	2.682252449942972E-4	2.4384113181299743E-5	0.0	8.53443961345491E-5	2.0320094317749786E-5
40	2.84481320448497E-4	2.4384113181299743E-5	0.0	9.347243386164902E-5	2.0320094317749786E-5
41	3.0886543362979675E-4	2.4384113181299743E-5	0.0	9.753645272519897E-5	2.0320094317749786E-5
42	3.251215090839966E-4	2.4384113181299743E-5	0.0	9.753645272519897E-5	2.0320094317749786E-5
43	3.332495468110965E-4	2.4384113181299743E-5	0.0	9.753645272519897E-5	2.0320094317749786E-5
44	3.6576169771949615E-4	2.84481320448497E-5	0.0	1.1785654704294876E-4	2.0320094317749786E-5
45	3.901458109007959E-4	2.84481320448497E-5	0.0	1.3004860363359863E-4	2.0320094317749786E-5
46	3.9420982976434585E-4	2.84481320448497E-5	0.0	1.422406602242485E-4	2.0320094317749786E-5
47	4.0233786749144576E-4	2.84481320448497E-5	0.0	1.5443271681489837E-4	2.0320094317749786E-5
48	4.267219806727455E-4	2.84481320448497E-5	0.0	1.625607545419983E-4	2.0320094317749786E-5
49	4.5923413158114516E-4	2.84481320448497E-5	0.0	1.625607545419983E-4	2.0320094317749786E-5
50	4.836182447624449E-4	2.84481320448497E-5	0.0	1.6662477340554825E-4	2.0320094317749786E-5
51	5.080023579437447E-4	2.84481320448497E-5	0.0	1.7881682999619812E-4	2.0320094317749786E-5
52	5.608346031698941E-4	2.84481320448497E-5	0.0	1.9507290545039795E-4	2.0320094317749786E-5
53	5.811546974876439E-4	2.84481320448497E-5	0.0	2.1132898090459777E-4	2.0320094317749786E-5
54	6.014747918053937E-4	2.84481320448497E-5	0.0	2.1539299976814773E-4	2.0320094317749786E-5
55	6.380509615773433E-4	2.84481320448497E-5	0.0	2.194570186316977E-4	2.0320094317749786E-5
56	6.543070370315431E-4	2.84481320448497E-5	0.0	2.3164907522234756E-4	2.0320094317749786E-5
57	6.908832068034927E-4	2.84481320448497E-5	0.0	2.479051506765474E-4	2.0320094317749786E-5
58	7.64035546347392E-4	2.84481320448497E-5	0.0	2.479051506765474E-4	2.0320094317749786E-5
59	8.371878858912912E-4	2.84481320448497E-5	4.064018863549957E-6	2.641612261307472E-4	2.0320094317749786E-5
60	8.778280745267908E-4	2.84481320448497E-5	4.064018863549957E-6	2.7228926385784713E-4	2.0320094317749786E-5
61	8.981481688445405E-4	2.84481320448497E-5	4.064018863549957E-6	2.7228926385784713E-4	2.0320094317749786E-5
62	9.347243386164902E-4	3.251215090839966E-5	4.064018863549957E-6	2.7228926385784713E-4	2.0320094317749786E-5
63	9.387883574800401E-4	3.251215090839966E-5	4.064018863549957E-6	2.84481320448497E-4	2.0320094317749786E-5
64	9.387883574800401E-4	3.251215090839966E-5	4.064018863549957E-6	2.8854533931204696E-4	2.0320094317749786E-5
65	9.428523763435901E-4	3.6576169771949615E-5	8.128037727099914E-6	2.926093581755969E-4	2.84481320448497E-5
>>END_MODULE
