##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543823_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24606185
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45352113706371	32.0	32.0	32.0	32.0	32.0
2	31.67111358384081	32.0	32.0	32.0	32.0	32.0
3	35.83427987719348	37.0	37.0	37.0	32.0	37.0
4	36.39822182918644	37.0	37.0	37.0	37.0	37.0
5	36.56093465931431	37.0	37.0	37.0	37.0	37.0
6	40.139628959141774	41.0	41.0	41.0	37.0	41.0
7	40.16933697767452	41.0	41.0	41.0	37.0	41.0
8	40.096318913313866	41.0	41.0	41.0	37.0	41.0
9	40.030051956449164	41.0	41.0	41.0	37.0	41.0
10-11	39.78029308484838	41.0	41.0	41.0	37.0	41.0
12-13	39.74079614535938	41.0	41.0	41.0	37.0	41.0
14-15	39.74188987443604	41.0	41.0	41.0	37.0	41.0
16-17	39.73216961507848	41.0	41.0	41.0	37.0	41.0
18-19	39.71448688612233	41.0	41.0	41.0	37.0	41.0
20-21	39.69802925565259	41.0	41.0	41.0	37.0	41.0
22-23	39.68044828972878	41.0	41.0	41.0	37.0	41.0
24-25	39.658985169785566	41.0	41.0	41.0	37.0	41.0
26-27	39.53580904557127	41.0	41.0	41.0	37.0	41.0
28-29	39.543449685516066	41.0	41.0	41.0	37.0	41.0
30-31	39.56937331000316	41.0	41.0	41.0	37.0	41.0
32-33	39.613479293925494	41.0	41.0	41.0	37.0	41.0
34-35	39.608958459021586	41.0	41.0	41.0	37.0	41.0
36-37	39.752837651225946	41.0	41.0	41.0	37.0	41.0
38-39	39.821587940470124	41.0	41.0	41.0	37.0	41.0
40-41	39.88164356459629	41.0	41.0	41.0	37.0	41.0
42-43	39.8753435033024	41.0	41.0	41.0	37.0	41.0
44-45	39.86382287264232	41.0	41.0	41.0	37.0	41.0
46-47	39.764176514017905	41.0	41.0	41.0	37.0	41.0
48-49	39.81566921477858	41.0	41.0	41.0	37.0	41.0
50-51	39.78612853807421	41.0	41.0	41.0	37.0	41.0
52-53	39.74495836186475	41.0	41.0	41.0	37.0	41.0
54-55	39.80749435111173	41.0	41.0	41.0	37.0	41.0
56-57	39.81475340307648	41.0	41.0	41.0	37.0	41.0
58-59	39.73771092479754	41.0	41.0	41.0	37.0	41.0
60-61	39.769611478788136	41.0	41.0	41.0	37.0	41.0
62-63	39.74349564598462	41.0	41.0	41.0	37.0	41.0
64-65	39.677523552454836	41.0	41.0	41.0	37.0	41.0
66-67	39.6685109937333	41.0	41.0	41.0	37.0	41.0
68-69	39.64487043013902	41.0	41.0	41.0	37.0	41.0
70-71	39.617772397380016	41.0	41.0	41.0	37.0	41.0
72-73	39.55581824540813	41.0	41.0	41.0	37.0	41.0
74-75	39.516658621265705	41.0	41.0	41.0	37.0	41.0
76	38.99865504241909	41.0	41.0	41.0	37.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	30211.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	1.0
15	0.0
16	8.0
17	27.0
18	94.0
19	213.0
20	499.0
21	1156.0
22	2713.0
23	5400.0
24	8921.0
25	14840.0
26	24116.0
27	38246.0
28	57147.0
29	81762.0
30	114030.0
31	154376.0
32	204802.0
33	270071.0
34	353500.0
35	467288.0
36	633600.0
37	913574.0
38	1544954.0
39	4442109.0
40	1.5242526E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.154542480543356	13.30391737606872	11.443521136437987	36.09801900694993
2	21.270360497002176	17.739154388219987	32.501606333583396	28.488878781194444
3	20.77892823291561	22.069416251823835	26.45049998832193	30.701155526938628
4	25.198629195470268	28.777861252919834	20.33897608473804	25.684533466871855
5	24.62860271580691	31.758586658661013	23.651404416362094	19.96140620916998
6	20.52555248371496	33.86326245747465	26.255358495516422	19.355826563293967
7	17.434922416503206	24.738128385064208	40.571262811394575	17.255686387038008
8	19.89178519115489	24.555494004826024	33.08863459675632	22.464086207262763
9	20.097189230424807	24.25462771078778	34.66742355765839	20.98075950112903
10-11	22.660444709129333	31.772559655214476	26.57219608061109	18.994799555045102
12-13	22.393983481890604	26.12007250769154	30.47229589033359	21.013648120084266
14-15	21.57542523274863	27.904503771750427	30.6096391454018	19.91043185009914
16-17	21.8584487349322	28.20260494054423	29.871547819418005	20.06739850510557
18-19	21.832466650098993	28.49803807356962	29.692208517892833	19.977286758438552
20-21	21.565100691950263	28.375331978229223	29.957981518655323	20.101585811165187
22-23	21.91659595363873	28.164111299796733	29.554068875235203	20.365223871329334
24-25	21.86556929028012	28.302918884927642	29.436510932947407	20.395000891844827
26-27	21.665745273476755	28.550196463274464	29.451011758903313	20.33304650434547
28-29	22.044962161134656	28.294987468707376	29.39596404291928	20.264086327238687
30-31	21.784991030177935	28.461493148537265	29.24125774272212	20.512258078562677
32-33	21.74382944566639	28.24711019213523	29.43876809841688	20.570292263781504
34-35	21.809529431968773	28.375871272197976	29.270755195266336	20.54384410056691
36-37	22.220524222404954	28.184402942968127	29.037897157951264	20.557175676675655
38-39	21.83040152030825	28.433808988032	29.378935606332686	20.35685388532707
40-41	22.165005660633	27.95330213905485	29.375446613521422	20.506245586790726
42-43	21.81569359895665	27.75989156075722	29.673167529614936	20.751247310671193
44-45	21.93565442322234	27.84911597538993	29.705334089815306	20.509895511572427
46-47	21.892326168990227	28.063967418018855	29.456928825983304	20.586777587007614
48-49	21.936746226930286	27.95489270298306	29.343405663549692	20.76495540653696
50-51	21.680956718565348	28.078094989788028	29.526238311476337	20.71470998017029
52-53	21.906628895031677	27.858495033029186	29.38103538321369	20.85384068872545
54-55	21.79371209422991	28.18697659180781	29.109326877221243	20.909984436741038
56-57	22.06058919822034	27.851616337948208	29.421065823321825	20.666728640509625
58-59	21.863924341611543	27.804186646846134	29.46379298321085	20.868096028331472
60-61	21.597252447343703	27.819891910437555	29.566537661586846	21.016317980631896
62-63	21.53844395806249	28.061712352127376	29.58023976229992	20.819603927510208
64-65	21.94014518696405	27.970638631071193	29.222750275745902	20.866465906218856
66-67	22.044301821447178	27.888252377998146	29.112621719577042	20.954824080977634
68-69	21.791888826319592	28.280234167443535	29.19477869412994	20.73309831210693
70-71	22.072438160876462	28.029606001474733	29.092152717936177	20.805803119712625
72-73	21.985509369536004	27.766887199250878	29.22554647137118	21.022056959841937
74-75	22.07417845385871	24.94360551410815	30.772362814290332	22.209853217742808
76	23.555209422367547	0.0	44.02190647246188	32.42288410517058
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1120905.0
1	690105.5
2	161918.5
3	58809.0
4	53088.0
5	58711.0
6	81966.0
7	119785.0
8	139972.0
9	140951.0
10	151559.0
11	136562.0
12	111936.0
13	93782.5
14	48539.5
15	18191.0
16	14243.5
17	14026.0
18	16111.5
19	21478.0
20	28445.0
21	34228.0
22	43564.5
23	58290.5
24	74735.0
25	90426.5
26	121817.5
27	168734.0
28	212807.0
29	249529.0
30	303702.5
31	367826.5
32	420937.0
33	462334.0
34	523127.5
35	620242.5
36	706484.0
37	746644.0
38	750469.5
39	785404.0
40	847874.0
41	883489.5
42	894320.0
43	931738.0
44	999411.5
45	1066671.0
46	1099197.0
47	1119232.5
48	1138578.0
49	1146338.5
50	1148377.5
51	1130666.5
52	1117485.5
53	1079116.0
54	1040887.5
55	993150.5
56	902744.0
57	799084.0
58	727277.0
59	661746.5
60	570513.0
61	469463.0
62	393855.0
63	338443.0
64	270225.0
65	215056.5
66	160250.5
67	126692.5
68	101209.0
69	72277.0
70	55922.0
71	47695.5
72	43115.5
73	37551.5
74	31124.0
75	25136.5
76	20637.5
77	16095.0
78	15905.5
79	18190.5
80	14494.5
81	10788.0
82	9659.0
83	8107.5
84	8439.5
85	7782.5
86	7434.5
87	8292.0
88	5723.5
89	3003.5
90	1720.0
91	564.5
92	352.0
93	300.0
94	225.0
95	226.5
96	250.5
97	174.5
98	58.0
99	9.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9276529457939131
2	0.17897532673187658
3	0.12277807388670775
4	0.13006079569018927
5	0.12277807388670775
6	0.12296501875443105
7	0.12277807388670775
8	0.12294063464124975
9	0.12277807388670775
10-11	0.12277807388670775
12-13	0.12317837974476743
14-15	0.12298330683931703
16-17	0.12301378698079364
18-19	0.12278416991500307
20-21	0.12278823393386663
22-23	0.12324950007487954
24-25	0.12485275551655
26-27	0.1336655804221581
28-29	0.13614666393835534
30-31	0.13619543216471794
32-33	0.14171843380028235
34-35	0.14750153264311391
36-37	4.3959210736793E-4
38-39	0.001422704176774515
40-41	4.0712096915635247E-4
42-43	4.071887048459284E-6
44-45	0.0
46-47	1.8330660553296325E-5
48-49	6.152442531908563E-4
50-51	4.992708404105824E-4
52-53	1.855104816276127E-4
54-55	1.223653416322052E-4
56-57	0.001738393205608497
58-59	0.0020802471995105885
60-61	1.2256301572346628E-5
62-63	0.0028311817816784354
64-65	0.0
66-67	0.0011262170028912449
68-69	0.0011970307979011705
70-71	0.0
72-73	6.1804799019034235E-6
74-75	6.381323082619862E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	37440.0
36	1030.0
37	1151.0
38	1340.0
39	1546.0
40	1908.0
41	1983.0
42	2298.0
43	2325.0
44	2306.0
45	2489.0
46	2671.0
47	2927.0
48	3346.0
49	3624.0
50	4040.0
51	4475.0
52	4740.0
53	5092.0
54	5416.0
55	5645.0
56	6010.0
57	6578.0
58	7047.0
59	7516.0
60	8075.0
61	8828.0
62	9177.0
63	9878.0
64	10422.0
65	10981.0
66	11703.0
67	12143.0
68	12687.0
69	13359.0
70	15156.0
71	39602.0
72	98542.0
73	435269.0
74	1655597.0
75	6342708.0
76	1.5787115E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.06417279888293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.4610375783995	17.159192758558227
2	14.454155967599025	7.245629256678135
3	5.429530575885647	4.082600777124486
4	2.84163703986416	2.848931271954459
5	1.7217128928053205	2.1576654727668574
6	1.1815230875380687	1.7768339299154299
7	0.8402783054509193	1.4742616452882058
8	0.6411568652855961	1.2856053170166626
9	0.5080936634052332	1.14614526398456
>10	3.2272523895090397	15.715427145081525
>50	0.3564726628514753	6.258859051063024
>100	0.28287781297203785	14.182501267608508
>500	0.03399936014655221	5.928233541876168
>1k	0.01926717675959988	8.507207059056936
>5k	6.235581897075703E-4	0.9277226479386289
>10k+	3.810633381546255E-4	9.303183594088322
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1023845	4.160925393351306	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	174627	0.7096874220851384	No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	146713	0.5962443995280049	No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	129732	0.527233295206063	No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	126979	0.51604505127471	No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92655	0.37655166780222127	No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	89600	0.36413609017407617	No Hit
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54825	0.2228098341941264	No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52254	0.21236124169593945	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38883	0.15802124547141297	No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35556	0.14450025471238226	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32421	0.13175955557515315	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	30211	0.12277807388670775	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.064018863549957E-6
2	0.0	0.0	0.0	0.0	4.064018863549957E-6
3	0.0	0.0	0.0	0.0	2.4384113181299743E-5
4	3.251215090839966E-5	0.0	0.0	4.064018863549957E-6	2.4384113181299743E-5
5	3.251215090839966E-5	0.0	0.0	8.128037727099914E-6	2.4384113181299743E-5
6	3.251215090839966E-5	0.0	0.0	8.128037727099914E-6	2.4384113181299743E-5
7	3.251215090839966E-5	0.0	0.0	1.625607545419983E-5	2.4384113181299743E-5
8	3.251215090839966E-5	0.0	0.0	1.625607545419983E-5	3.251215090839966E-5
9	3.251215090839966E-5	0.0	0.0	3.251215090839966E-5	5.2832245226149444E-5
10	3.6576169771949615E-5	0.0	0.0	3.251215090839966E-5	5.2832245226149444E-5
11	6.502430181679932E-5	0.0	0.0	3.251215090839966E-5	5.68962640896994E-5
12	6.908832068034927E-5	0.0	0.0	3.251215090839966E-5	6.908832068034927E-5
13	6.908832068034927E-5	0.0	0.0	3.6576169771949615E-5	6.908832068034927E-5
14	6.908832068034927E-5	0.0	0.0	3.6576169771949615E-5	6.908832068034927E-5
15	7.315233954389923E-5	0.0	0.0	3.6576169771949615E-5	6.908832068034927E-5
16	7.315233954389923E-5	0.0	0.0	4.064018863549957E-5	6.908832068034927E-5
17	7.315233954389923E-5	4.064018863549957E-6	0.0	4.064018863549957E-5	6.908832068034927E-5
18	7.721635840744919E-5	1.2192056590649872E-5	0.0	4.064018863549957E-5	6.908832068034927E-5
19	7.721635840744919E-5	1.2192056590649872E-5	4.064018863549957E-6	4.064018863549957E-5	6.908832068034927E-5
20	8.128037727099914E-5	1.2192056590649872E-5	8.128037727099914E-6	4.470420749904953E-5	6.908832068034927E-5
21	8.940841499809906E-5	1.2192056590649872E-5	8.128037727099914E-6	4.470420749904953E-5	6.908832068034927E-5
22	8.940841499809906E-5	1.2192056590649872E-5	8.128037727099914E-6	4.470420749904953E-5	6.908832068034927E-5
23	9.347243386164902E-5	1.625607545419983E-5	8.128037727099914E-6	4.8768226362599486E-5	6.908832068034927E-5
24	9.753645272519897E-5	1.625607545419983E-5	1.2192056590649872E-5	4.8768226362599486E-5	6.908832068034927E-5
25	1.0160047158874893E-4	1.625607545419983E-5	1.2192056590649872E-5	5.2832245226149444E-5	6.908832068034927E-5
26	1.3004860363359863E-4	1.625607545419983E-5	1.2192056590649872E-5	5.2832245226149444E-5	6.908832068034927E-5
27	1.3004860363359863E-4	1.625607545419983E-5	1.2192056590649872E-5	5.2832245226149444E-5	6.908832068034927E-5
28	1.341126224971486E-4	1.625607545419983E-5	1.2192056590649872E-5	5.2832245226149444E-5	6.908832068034927E-5
29	1.3817664136069855E-4	1.625607545419983E-5	1.2192056590649872E-5	5.2832245226149444E-5	6.908832068034927E-5
30	1.3817664136069855E-4	1.625607545419983E-5	1.2192056590649872E-5	5.2832245226149444E-5	6.908832068034927E-5
31	1.5849673567844833E-4	1.625607545419983E-5	1.2192056590649872E-5	6.096028295324936E-5	6.908832068034927E-5
32	1.5849673567844833E-4	1.625607545419983E-5	1.2192056590649872E-5	6.096028295324936E-5	8.128037727099914E-5
33	1.7881682999619812E-4	1.625607545419983E-5	1.2192056590649872E-5	6.502430181679932E-5	8.53443961345491E-5
34	1.7881682999619812E-4	2.4384113181299743E-5	1.2192056590649872E-5	6.502430181679932E-5	8.940841499809906E-5
35	1.91008886586848E-4	2.4384113181299743E-5	1.2192056590649872E-5	6.502430181679932E-5	9.347243386164902E-5
36	1.91008886586848E-4	2.4384113181299743E-5	1.2192056590649872E-5	7.315233954389923E-5	9.753645272519897E-5
37	1.91008886586848E-4	2.4384113181299743E-5	1.2192056590649872E-5	7.315233954389923E-5	9.753645272519897E-5
38	1.9507290545039795E-4	2.4384113181299743E-5	1.2192056590649872E-5	7.315233954389923E-5	1.137925281793988E-4
39	1.9507290545039795E-4	2.4384113181299743E-5	1.2192056590649872E-5	7.315233954389923E-5	1.2598458477004867E-4
40	2.0726496204104782E-4	2.4384113181299743E-5	1.625607545419983E-5	7.721635840744919E-5	1.3004860363359863E-4
41	2.1132898090459777E-4	2.4384113181299743E-5	1.625607545419983E-5	8.53443961345491E-5	1.4630467908779846E-4
42	2.194570186316977E-4	2.4384113181299743E-5	1.625607545419983E-5	1.0566449045229889E-4	1.5036869795134842E-4
43	2.194570186316977E-4	2.4384113181299743E-5	1.625607545419983E-5	1.0972850931584884E-4	1.706887922690982E-4
44	2.3571309408589752E-4	2.84481320448497E-5	1.625607545419983E-5	1.0972850931584884E-4	1.706887922690982E-4
45	2.3571309408589752E-4	2.84481320448497E-5	1.625607545419983E-5	1.137925281793988E-4	1.7881682999619812E-4
46	2.682252449942972E-4	2.84481320448497E-5	1.625607545419983E-5	1.2192056590649872E-4	1.8288084885974807E-4
47	2.84481320448497E-4	2.84481320448497E-5	2.0320094317749786E-5	1.2598458477004867E-4	1.91008886586848E-4
48	3.0073739590269683E-4	2.84481320448497E-5	2.0320094317749786E-5	1.3004860363359863E-4	1.9507290545039795E-4
49	3.1699347135689666E-4	2.84481320448497E-5	2.0320094317749786E-5	1.3004860363359863E-4	1.991369243139479E-4
50	3.251215090839966E-4	2.84481320448497E-5	2.0320094317749786E-5	1.3004860363359863E-4	2.0726496204104782E-4
51	3.3731356567464645E-4	2.84481320448497E-5	2.0320094317749786E-5	1.3004860363359863E-4	2.0726496204104782E-4
52	3.413775845381964E-4	2.84481320448497E-5	2.0320094317749786E-5	1.341126224971486E-4	2.0726496204104782E-4
53	3.495056222652963E-4	2.84481320448497E-5	2.0320094317749786E-5	1.341126224971486E-4	2.1132898090459777E-4
54	3.535696411288463E-4	2.84481320448497E-5	2.0320094317749786E-5	1.3817664136069855E-4	2.1132898090459777E-4
55	3.6576169771949615E-4	2.84481320448497E-5	2.0320094317749786E-5	1.3817664136069855E-4	2.1132898090459777E-4
56	3.7388973544659606E-4	2.84481320448497E-5	2.0320094317749786E-5	1.3817664136069855E-4	2.1132898090459777E-4
57	3.77953754310146E-4	3.251215090839966E-5	2.0320094317749786E-5	1.3817664136069855E-4	2.1132898090459777E-4
58	4.1452992408209564E-4	3.251215090839966E-5	2.0320094317749786E-5	1.3817664136069855E-4	2.1539299976814773E-4
59	4.673621693082451E-4	3.251215090839966E-5	2.0320094317749786E-5	1.4630467908779846E-4	2.1539299976814773E-4
60	4.7955422589889495E-4	3.251215090839966E-5	2.0320094317749786E-5	1.4630467908779846E-4	2.1539299976814773E-4
61	4.836182447624449E-4	3.251215090839966E-5	2.0320094317749786E-5	1.5036869795134842E-4	2.1539299976814773E-4
62	4.836182447624449E-4	3.251215090839966E-5	2.0320094317749786E-5	1.5036869795134842E-4	2.1539299976814773E-4
63	4.836182447624449E-4	3.251215090839966E-5	2.0320094317749786E-5	1.5036869795134842E-4	2.1539299976814773E-4
64	4.836182447624449E-4	3.251215090839966E-5	2.0320094317749786E-5	1.5036869795134842E-4	2.1539299976814773E-4
65	4.8768226362599486E-4	3.6576169771949615E-5	2.4384113181299743E-5	1.5443271681489837E-4	2.194570186316977E-4
>>END_MODULE
