##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543822_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26680950
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.446936634565112	32.0	32.0	32.0	27.0	32.0
2	30.855899246466112	32.0	32.0	32.0	32.0	32.0
3	32.409582679777145	37.0	32.0	37.0	22.0	37.0
4	34.6600968106458	37.0	37.0	37.0	27.0	37.0
5	35.34963751290715	37.0	37.0	37.0	32.0	37.0
6	38.59694587336658	41.0	41.0	41.0	32.0	41.0
7	38.24313961834193	41.0	37.0	41.0	32.0	41.0
8	38.157558257858135	41.0	37.0	41.0	32.0	41.0
9	38.43681975341958	41.0	41.0	41.0	32.0	41.0
10-11	38.55404691362189	41.0	41.0	41.0	34.5	41.0
12-13	38.728138053555064	41.0	41.0	41.0	34.5	41.0
14-15	38.605484943377206	41.0	41.0	41.0	32.0	41.0
16-17	38.450924517305424	41.0	41.0	41.0	32.0	41.0
18-19	38.564448304876706	41.0	41.0	41.0	34.5	41.0
20-21	38.663702079573625	41.0	41.0	41.0	34.5	41.0
22-23	38.431430046531325	41.0	39.0	41.0	32.0	41.0
24-25	38.49411694486141	41.0	39.0	41.0	32.0	41.0
26-27	38.7277325020286	41.0	41.0	41.0	34.5	41.0
28-29	38.384841581727784	41.0	39.0	41.0	32.0	41.0
30-31	38.5259893294654	41.0	41.0	41.0	32.0	41.0
32-33	38.739575408671726	41.0	41.0	41.0	34.5	41.0
34-35	38.65039724972311	41.0	41.0	41.0	34.5	41.0
36-37	38.415586813055924	41.0	39.0	41.0	32.0	41.0
38-39	38.35944423789455	41.0	37.0	41.0	32.0	41.0
40-41	38.39880550947461	41.0	37.0	41.0	32.0	41.0
42-43	38.37719604792761	41.0	37.0	41.0	32.0	41.0
44-45	38.34191707198809	41.0	37.0	41.0	32.0	41.0
46-47	38.359390360478784	41.0	37.0	41.0	32.0	41.0
48-49	38.34500526511292	41.0	37.0	41.0	32.0	41.0
50-51	38.29362548190234	41.0	37.0	41.0	32.0	41.0
52-53	38.32497950469176	41.0	37.0	41.0	32.0	41.0
54-55	38.33262508273238	41.0	37.0	41.0	32.0	41.0
56-57	38.331342562158746	41.0	37.0	41.0	32.0	41.0
58-59	38.35023205454021	41.0	37.0	41.0	32.0	41.0
60-61	38.32617247486435	41.0	37.0	41.0	32.0	41.0
62-63	38.36594495774375	41.0	37.0	41.0	32.0	41.0
64-65	38.35756717042234	41.0	37.0	41.0	32.0	41.0
66-67	38.28476604136905	41.0	37.0	41.0	32.0	41.0
68-69	38.30427010735424	41.0	37.0	41.0	32.0	41.0
70-71	38.13249312913342	41.0	37.0	41.0	32.0	41.0
72-73	38.245530521046646	41.0	37.0	41.0	32.0	41.0
74-75	38.2750415166047	41.0	37.0	41.0	32.0	41.0
76	37.33725258972322	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27604.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	38.0
13	301.0
14	1295.0
15	4872.0
16	13652.0
17	28898.0
18	45018.0
19	58819.0
20	70415.0
21	80908.0
22	91801.0
23	103545.0
24	118679.0
25	135181.0
26	155237.0
27	178023.0
28	205567.0
29	239684.0
30	278812.0
31	327291.0
32	391422.0
33	472985.0
34	587597.0
35	756531.0
36	1031165.0
37	1538575.0
38	2784523.0
39	7175100.0
40	9777412.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.3124677977567	18.611492401864567	14.60491471889586	30.47112508148287
2	22.88932319878998	26.169688365759985	28.679191237802126	22.261797197647905
3	22.301200006933463	27.329352945029868	27.799012551744912	22.57043449629176
4	26.178697131245492	31.733862904142608	19.278824959113468	22.808615005498435
5	25.354832486244238	33.84210206213289	19.968823359270846	20.834242092352028
6	20.755971035934046	35.99638301854144	20.756807709583747	22.490838235940767
7	20.97776824298738	21.090624943604478	33.21517897110482	24.71642784230332
8	21.750133925861313	23.196461941005385	25.822631958526244	29.230772174607054
9	22.554252416169003	23.214015975098988	27.05353260247637	27.178199006255642
10-11	24.44201537614242	30.12804434934776	21.136023943773314	24.2939163307365
12-13	24.173440042513285	24.477745535175373	25.07096443858793	26.27784998372341
14-15	23.40811330575127	27.254701289535955	25.631764374943078	23.7054210297697
16-17	23.674878185226593	27.195624773935506	25.036858326462486	24.09263871437542
18-19	23.51147339183162	27.52100683872823	25.104777887424834	23.86274188201532
20-21	23.5155418056088	27.525971012866922	25.18385103083486	23.77463615068942
22-23	23.710876383785642	27.16265896799125	25.04362789923913	24.08283674898398
24-25	23.560575490834065	27.076806259214838	25.286020289288285	24.07659796066281
26-27	23.38384601763427	27.405679932867788	25.166803892793393	24.043670156704554
28-29	23.910629168787693	26.765839643824208	25.068540442491223	24.25499074489688
30-31	23.57057916827008	27.083720173399655	25.331977022108127	24.01372363622214
32-33	23.423309560655174	27.289781659854036	25.392191587200447	23.894717192290344
34-35	23.707467021759122	27.106009013065503	24.989557612817375	24.196966352358004
36-37	23.440360687681885	26.911837194002153	25.44077349603014	24.207028622285822
38-39	23.245060725934348	27.20312373816658	25.54857233017091	24.003243205728168
40-41	23.658143622642413	26.908143477179507	25.187784636890893	24.245928263287187
42-43	23.272893415968717	26.96721054964255	25.453778088214168	24.306117946174563
44-45	23.38375013702021	26.85065722573154	25.444971612641616	24.320621024606634
46-47	23.617897066615527	26.690275237641625	25.243807060103034	24.448020635639814
48-49	23.441636846463027	26.727234309894126	25.489218402462633	24.341910441180218
50-51	23.271935213048174	26.91330090543282	25.49767555950737	24.31708832201164
52-53	23.501927792858808	26.787050812553137	25.228697777535753	24.48232361705231
54-55	23.402450802877592	26.611309373968844	25.616941238600226	24.369298584553338
56-57	23.274015505599365	26.956607584653714	25.382271121343297	24.387105788403627
58-59	23.469956891030748	26.92150762492388	25.325990448692764	24.282545035352605
60-61	23.369051367974066	26.71427221115406	25.605973484058065	24.31070293681381
62-63	23.22282825685597	27.096986235469277	25.46615759254742	24.214027915127335
64-65	23.60945568755151	26.828208501729634	25.325006029522555	24.2373297811963
66-67	23.389348404224176	26.808475170041994	25.603482970189003	24.198693455544827
68-69	23.237709396363996	26.905374719473546	25.798768057533078	24.058147826629376
70-71	23.546704607415485	26.912092572397917	25.428440794256634	24.112762025929964
72-73	23.286102919353805	26.716140634840812	25.59896477330792	24.398791672497467
74-75	23.247992123555928	23.528640607648537	27.308453962098696	25.91491330669684
76	24.509955898013718	0.0	38.11558196309243	37.37446213889385
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64389.0
1	38394.0
2	11311.5
3	9502.5
4	8781.5
5	8348.5
6	8856.0
7	10875.5
8	11963.5
9	13133.5
10	16821.5
11	39071.0
12	58803.5
13	75543.5
14	71142.5
15	41163.5
16	25091.5
17	18568.0
18	18610.5
19	20134.0
20	25664.0
21	31486.0
22	39291.5
23	53071.0
24	72745.0
25	90953.5
26	109384.0
27	141618.0
28	182726.5
29	210823.0
30	258133.0
31	335911.0
32	402257.0
33	442427.5
34	519995.5
35	626173.0
36	694193.5
37	729242.0
38	758179.0
39	823292.0
40	893870.0
41	944677.5
42	978720.5
43	1045468.0
44	1155187.0
45	1235881.0
46	1275407.5
47	1334254.5
48	1387492.5
49	1403527.0
50	1405457.5
51	1401305.0
52	1405923.0
53	1389699.5
54	1357490.0
55	1282300.5
56	1161852.0
57	1030988.5
58	943283.0
59	859819.0
60	742974.0
61	627383.5
62	541155.0
63	462362.5
64	369802.5
65	294430.5
66	225595.0
67	189261.5
68	164784.0
69	131773.0
70	103162.5
71	86732.5
72	78797.0
73	74262.0
74	61329.0
75	46367.0
76	37868.0
77	29508.5
78	24707.5
79	22088.0
80	20920.5
81	19311.0
82	17163.5
83	15775.0
84	14366.0
85	11442.5
86	8743.5
87	7733.5
88	7278.0
89	4726.0
90	2032.0
91	1211.0
92	1025.5
93	922.0
94	745.0
95	626.0
96	581.0
97	542.0
98	454.5
99	349.0
100	292.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.2437862969646882
2	0.2418317188855719
3	0.10345958446007358
4	0.10464395008423612
5	0.106079431204661
6	0.10414171909171151
7	0.10374817988115115
8	0.10548350040009818
9	0.10576834782869426
10-11	0.10434785867819549
12-13	0.10357764622324168
14-15	0.10403490130598798
16-17	0.10449965237369734
18-19	0.10592014152419611
20-21	0.10463458010303232
22-23	0.10384562768567086
24-25	0.10981805370498428
26-27	0.10670347195283526
28-29	0.1093176967087004
30-31	0.11515519499867882
32-33	0.12286481553318003
34-35	0.13022025077817695
36-37	0.002865443609514684
38-39	0.004076174543970935
40-41	0.0011186441702318913
42-43	0.0022564470888969757
44-45	0.0014588989578786205
46-47	2.0470034433039296E-4
48-49	0.004094999171983654
50-51	0.003932743435597314
52-53	0.0017086520765197935
54-55	0.009816315487353266
56-57	0.004285875335287903
58-59	0.004180903496445733
60-61	0.0010322335261839406
62-63	3.581653113879792E-4
64-65	6.999725293799791E-4
66-67	0.005163567424499824
68-69	0.0036567387218775474
70-71	0.0038090757932627522
72-73	0.0015007526017515019
74-75	2.7823699292831234E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	35327.0
36	1068.0
37	1229.0
38	1397.0
39	1567.0
40	1941.0
41	2363.0
42	2531.0
43	2583.0
44	2545.0
45	2674.0
46	2883.0
47	3241.0
48	3538.0
49	3895.0
50	4490.0
51	5017.0
52	5492.0
53	5657.0
54	6249.0
55	6231.0
56	6728.0
57	7238.0
58	7643.0
59	8429.0
60	9226.0
61	10274.0
62	10858.0
63	11451.0
64	12290.0
65	13464.0
66	14972.0
67	14525.0
68	15211.0
69	16377.0
70	19480.0
71	46180.0
72	135750.0
73	616174.0
74	1966924.0
75	6489218.0
76	1.714662E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.8034621676602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.42638004329926	23.352130998153083
2	12.062069416095703	7.672311366769819
3	4.5719400326441955	4.362105655830325
4	2.471725244001739	3.1443768114584
5	1.4766417345376732	2.3481159769778515
6	0.9613351255671907	1.834427117785141
7	0.709438424725072	1.5793818670719797
8	0.5187336770556598	1.3198021498664754
9	0.41252323240307237	1.180770031351081
>10	2.8100491174053737	17.3326846580302
>50	0.3061477326163231	6.771033726438648
>100	0.23221434606861768	14.968721088863985
>500	0.02732877915141994	5.9790329091587315
>1k	0.013199122324880722	6.87435522613189
>5k	1.9925243897697372E-4	0.4178088067929617
>10k+	7.471966461619281E-5	0.862941609319263
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82666	0.3098315464779178	No Hit
CCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38081	0.1427273016890328	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	27604	0.10345958446007358	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.747992481527082E-6	0.0
2	0.0	3.747992481527082E-6	0.0	7.495984963054164E-6	0.0
3	0.0	3.747992481527082E-6	0.0	7.495984963054164E-6	0.0
4	3.747992481527082E-6	3.747992481527082E-6	0.0	7.495984963054164E-6	0.0
5	3.747992481527082E-6	3.747992481527082E-6	0.0	7.495984963054164E-6	0.0
6	3.747992481527082E-6	3.747992481527082E-6	0.0	7.495984963054164E-6	0.0
7	3.747992481527082E-6	3.747992481527082E-6	0.0	4.8723902259852064E-5	0.0
8	7.495984963054164E-6	3.747992481527082E-6	0.0	4.8723902259852064E-5	0.0
9	1.1243977444581246E-5	3.747992481527082E-6	0.0	5.247189474137915E-5	0.0
10	1.1243977444581246E-5	3.747992481527082E-6	0.0	5.247189474137915E-5	3.747992481527082E-6
11	1.1243977444581246E-5	3.747992481527082E-6	0.0	5.247189474137915E-5	3.747992481527082E-6
12	2.6235947370689576E-5	3.747992481527082E-6	0.0	5.247189474137915E-5	7.495984963054164E-6
13	2.9983939852216656E-5	3.747992481527082E-6	0.0	5.996787970443331E-5	7.495984963054164E-6
14	4.8723902259852064E-5	3.747992481527082E-6	0.0	5.996787970443331E-5	7.495984963054164E-6
15	5.996787970443331E-5	3.747992481527082E-6	0.0	5.996787970443331E-5	7.495984963054164E-6
16	9.369981203817706E-5	3.747992481527082E-6	0.0	5.996787970443331E-5	7.495984963054164E-6
17	1.049437894827583E-4	3.747992481527082E-6	0.0	5.996787970443331E-5	7.495984963054164E-6
18	1.0869178196428538E-4	3.747992481527082E-6	0.0	5.996787970443331E-5	7.495984963054164E-6
19	1.1993575940886662E-4	3.747992481527082E-6	0.0	5.996787970443331E-5	7.495984963054164E-6
20	1.1993575940886662E-4	3.747992481527082E-6	0.0	7.870784211206872E-5	7.495984963054164E-6
21	1.3117973685344787E-4	3.747992481527082E-6	0.0	8.245583459359581E-5	7.495984963054164E-6
22	1.3492772933497495E-4	3.747992481527082E-6	0.0	8.245583459359581E-5	7.495984963054164E-6
23	1.4242371429802912E-4	3.747992481527082E-6	0.0	8.245583459359581E-5	7.495984963054164E-6
24	1.5741568422413744E-4	3.747992481527082E-6	0.0	8.245583459359581E-5	7.495984963054164E-6
25	1.83651631594827E-4	3.747992481527082E-6	0.0	8.245583459359581E-5	7.495984963054164E-6
26	2.061395864839895E-4	3.747992481527082E-6	0.0	8.245583459359581E-5	7.495984963054164E-6
27	2.1738356392857075E-4	3.747992481527082E-6	0.0	8.245583459359581E-5	7.495984963054164E-6
28	2.3237553385467907E-4	7.495984963054164E-6	0.0	8.620382707512288E-5	7.495984963054164E-6
29	2.511154962623145E-4	7.495984963054164E-6	0.0	8.620382707512288E-5	7.495984963054164E-6
30	2.73603451151477E-4	7.495984963054164E-6	0.0	8.620382707512288E-5	7.495984963054164E-6
31	2.923434135591124E-4	7.495984963054164E-6	0.0	8.620382707512288E-5	7.495984963054164E-6
32	3.148313684482749E-4	7.495984963054164E-6	0.0	8.620382707512288E-5	1.1243977444581246E-5
33	3.335713308559103E-4	7.495984963054164E-6	0.0	8.620382707512288E-5	1.1243977444581246E-5
34	3.63555270708127E-4	7.495984963054164E-6	0.0	8.995181955664997E-5	1.1243977444581246E-5
35	3.972872030418707E-4	7.495984963054164E-6	0.0	8.995181955664997E-5	1.1243977444581246E-5
36	4.12279172967979E-4	7.495984963054164E-6	0.0	1.0119579700123122E-4	1.1243977444581246E-5
37	4.2352315041256026E-4	7.495984963054164E-6	0.0	1.0119579700123122E-4	1.1243977444581246E-5
38	4.5350709026477696E-4	7.495984963054164E-6	0.0	1.049437894827583E-4	1.1243977444581246E-5
39	4.909870150800478E-4	7.495984963054164E-6	0.0	1.049437894827583E-4	1.1243977444581246E-5
40	5.284669398953186E-4	7.495984963054164E-6	0.0	1.0869178196428538E-4	1.1243977444581246E-5
41	5.621988722290623E-4	7.495984963054164E-6	0.0	1.1243977444581246E-4	1.1243977444581246E-5
42	5.92182812081279E-4	7.495984963054164E-6	0.0	1.1618776692733954E-4	1.1243977444581246E-5
43	6.259147444150227E-4	7.495984963054164E-6	0.0	1.1993575940886662E-4	1.1243977444581246E-5
44	6.596466767487665E-4	7.495984963054164E-6	0.0	1.1993575940886662E-4	1.1243977444581246E-5
45	6.933786090825102E-4	7.495984963054164E-6	0.0	1.236837518903937E-4	1.1243977444581246E-5
46	7.158665639716726E-4	7.495984963054164E-6	0.0	1.236837518903937E-4	1.4991969926108328E-5
47	7.495984963054164E-4	7.495984963054164E-6	0.0	1.274317443719208E-4	1.4991969926108328E-5
48	7.833304286391601E-4	7.495984963054164E-6	0.0	1.3867572181650205E-4	1.4991969926108328E-5
49	8.24558345935958E-4	7.495984963054164E-6	0.0	1.4242371429802912E-4	1.4991969926108328E-5
50	8.320543308990122E-4	7.495984963054164E-6	0.0	1.4242371429802912E-4	1.4991969926108328E-5
51	8.657862632327559E-4	7.495984963054164E-6	0.0	1.499196992610833E-4	1.4991969926108328E-5
52	8.995181955664997E-4	7.495984963054164E-6	0.0	1.5366769174261037E-4	1.8739962407635412E-5
53	9.182581579741351E-4	7.495984963054164E-6	0.0	1.5741568422413744E-4	1.8739962407635412E-5
54	9.444941053448247E-4	7.495984963054164E-6	0.0	1.5741568422413744E-4	1.8739962407635412E-5
55	9.707300527155143E-4	7.495984963054164E-6	0.0	1.6116367670566454E-4	1.8739962407635412E-5
56	0.001008209977530785	7.495984963054164E-6	0.0	1.6491166918719162E-4	1.8739962407635412E-5
57	0.001045689902346056	7.495984963054164E-6	0.0	1.873996240763541E-4	1.8739962407635412E-5
58	0.0011019097895689622	7.495984963054164E-6	0.0	1.873996240763541E-4	1.8739962407635412E-5
59	0.0011543816843103414	7.495984963054164E-6	0.0	1.873996240763541E-4	1.8739962407635412E-5
60	0.0012255935414593557	7.495984963054164E-6	0.0	1.9114761655788118E-4	1.8739962407635412E-5
61	0.0012555774813115725	7.495984963054164E-6	0.0	1.9114761655788118E-4	1.8739962407635412E-5
62	0.001278065436200735	7.495984963054164E-6	0.0	1.9489560903940826E-4	1.8739962407635412E-5
63	0.001278065436200735	7.495984963054164E-6	0.0	1.9489560903940826E-4	1.8739962407635412E-5
64	0.001278065436200735	1.1243977444581246E-5	0.0	1.9489560903940826E-4	1.8739962407635412E-5
65	0.0012818134286822621	1.4991969926108328E-5	0.0	1.9864360152093536E-4	2.2487954889162492E-5
>>END_MODULE
