##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543822_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26680950
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.461846523455876	32.0	32.0	32.0	32.0	32.0
2	31.68587325413825	32.0	32.0	32.0	32.0	32.0
3	35.928787768051734	37.0	37.0	37.0	32.0	37.0
4	36.411033527666746	37.0	37.0	37.0	37.0	37.0
5	36.56401623630343	37.0	37.0	37.0	37.0	37.0
6	40.13887923780825	41.0	41.0	41.0	37.0	41.0
7	40.20837605107764	41.0	41.0	41.0	37.0	41.0
8	40.26705840684084	41.0	41.0	41.0	41.0	41.0
9	40.15422164503138	41.0	41.0	41.0	37.0	41.0
10-11	39.94592941780559	41.0	41.0	41.0	37.0	41.0
12-13	39.79568051362489	41.0	41.0	41.0	37.0	41.0
14-15	39.83746427694666	41.0	41.0	41.0	37.0	41.0
16-17	39.82702349429087	41.0	41.0	41.0	37.0	41.0
18-19	39.776219343764	41.0	41.0	41.0	37.0	41.0
20-21	39.73232664129276	41.0	41.0	41.0	37.0	41.0
22-23	39.70100043289313	41.0	41.0	41.0	37.0	41.0
24-25	39.66795269658689	41.0	41.0	41.0	37.0	41.0
26-27	39.55267173770049	41.0	41.0	41.0	37.0	41.0
28-29	39.55626967930302	41.0	41.0	41.0	37.0	41.0
30-31	39.589131121642964	41.0	41.0	41.0	37.0	41.0
32-33	39.646888697741275	41.0	41.0	41.0	37.0	41.0
34-35	39.644384401604896	41.0	41.0	41.0	37.0	41.0
36-37	39.79839168337189	41.0	41.0	41.0	37.0	41.0
38-39	39.87076079271287	41.0	41.0	41.0	37.0	41.0
40-41	39.93632070570265	41.0	41.0	41.0	37.0	41.0
42-43	39.94686682179963	41.0	41.0	41.0	37.0	41.0
44-45	39.95145617911034	41.0	41.0	41.0	37.0	41.0
46-47	39.87137260948529	41.0	41.0	41.0	37.0	41.0
48-49	39.93891820400377	41.0	41.0	41.0	37.0	41.0
50-51	39.91832205381235	41.0	41.0	41.0	37.0	41.0
52-53	39.86726117495752	41.0	41.0	41.0	37.0	41.0
54-55	39.90184784410176	41.0	41.0	41.0	37.0	41.0
56-57	39.90227757076744	41.0	41.0	41.0	37.0	41.0
58-59	39.83154086303665	41.0	41.0	41.0	37.0	41.0
60-61	39.86684397486067	41.0	41.0	41.0	37.0	41.0
62-63	39.84505411386017	41.0	41.0	41.0	37.0	41.0
64-65	39.78391158154329	41.0	41.0	41.0	37.0	41.0
66-67	39.783820004026	41.0	41.0	41.0	37.0	41.0
68-69	39.77292331793761	41.0	41.0	41.0	37.0	41.0
70-71	39.755045834510014	41.0	41.0	41.0	37.0	41.0
72-73	39.70172126526704	41.0	41.0	41.0	37.0	41.0
74-75	39.6764680641978	41.0	41.0	41.0	37.0	41.0
76	39.193361394764885	41.0	41.0	41.0	37.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28676.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	1.0
13	2.0
14	1.0
15	3.0
16	6.0
17	17.0
18	76.0
19	206.0
20	450.0
21	972.0
22	2315.0
23	4443.0
24	7684.0
25	13252.0
26	21518.0
27	34545.0
28	53115.0
29	78277.0
30	111622.0
31	153406.0
32	208220.0
33	276947.0
34	366069.0
35	485086.0
36	656701.0
37	943722.0
38	1577402.0
39	4450712.0
40	1.7205504E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.232015958270296	10.031393126389172	12.50193991947864	36.2346509958619
2	23.153956091684076	14.382834740494243	32.63714304882232	29.82606611899936
3	22.587817459778478	19.339745644217825	27.023397703325426	31.04903919267827
4	27.402259818107627	26.118067757020302	20.408459543252206	26.071212881619864
5	26.81771168944158	29.41285235173554	23.71666297592468	20.052772982898194
6	22.36261987034108	31.704054087771627	26.014681320347055	19.91864472154024
7	19.077873055034626	22.165418230354376	40.89575245999647	17.86095625461452
8	21.59184692796507	21.793255920441347	32.63613229299035	23.97876485860323
9	21.631854002401447	21.4678417308782	34.24807954473228	22.652224721988077
10-11	24.47158917846935	29.11783249714452	25.597425570516048	20.81315275387008
12-13	24.134769420426363	23.305732927152125	29.527857120988088	23.03164053143342
14-15	23.34388211201871	25.195313492969714	29.652164201802876	21.808640193208696
16-17	23.688168972595456	25.445749729473587	28.938522850465255	21.927558447465703
18-19	23.696693169285776	25.721908918720114	28.721414983334537	21.859982928659573
20-21	23.376400082379472	25.61771002553591	29.01431739156487	21.991572500519748
22-23	23.74099843498678	25.404221292141642	28.594394805586383	22.2603854672852
24-25	23.68820329856811	25.514764466076024	28.51979491873647	22.2772373166194
26-27	23.496108504673053	25.791541391782385	28.48469811473401	22.227651988810553
28-29	23.825879928907945	25.579304066231202	28.429911498175027	22.16490450668583
30-31	23.564524748602178	25.664287145465213	28.367244460862917	22.403943645069692
32-33	23.505082420866035	25.470258136250916	28.55303093103994	22.471628511843107
34-35	23.623813009113316	25.59292288803558	28.368241302436896	22.41502280041421
36-37	24.005545999957665	25.380874647219787	28.148415624821254	22.465163728001293
38-39	23.63727028968222	25.670244489457865	28.435253206142985	22.257232014716923
40-41	23.94168738543363	25.214477247021133	28.425629605882314	22.418205761662918
42-43	23.62331681276338	25.01056635394314	28.80249165082511	22.563625182468364
44-45	23.730662696409198	25.105441891582615	28.806419661929894	22.357475750078294
46-47	23.739524809108374	25.30576774709146	28.524378082300757	22.430329361499414
48-49	23.783931808736433	25.16809661025113	28.4647644633716	22.583207117640843
50-51	23.5734569713467	25.401511998139597	28.539079506512238	22.485951524001464
52-53	23.77457399609657	25.18858531329248	28.40993775299763	22.626902937613313
54-55	23.63064218431987	25.39225252740205	28.24057598371354	22.736529304564538
56-57	23.863008142341503	25.130052006503366	28.48710958833464	22.51983026282049
58-59	23.784068062132473	25.03400695136931	28.492109026232438	22.689815960265776
60-61	23.474254298331072	25.09072775296049	28.605221109217428	22.829796839491003
62-63	23.39230767882162	25.28375986708787	28.7006378386618	22.623294615428712
64-65	23.803917799609493	25.219777474693682	28.33548722277164	22.640817502925184
66-67	23.856706289272662	25.123237139021086	28.227356916775907	22.792699654930345
68-69	23.612310611156108	25.463293559204853	28.316052812441622	22.60834301719742
70-71	23.93588693928222	25.27210132374149	28.15900178079005	22.63300995618624
72-73	23.85541199776425	24.948328700869585	28.316841508903074	22.879417792463087
74-75	24.01095108143089	21.7713413551696	29.97057373451122	24.24713382888829
76	24.789115721554822	0.0	41.264891164443995	33.94599311400118
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	286106.0
1	203952.5
2	78229.5
3	32282.0
4	29904.0
5	35202.5
6	50351.5
7	69994.5
8	79783.0
9	79795.5
10	87479.0
11	85278.5
12	75412.0
13	59921.0
14	29175.0
15	11927.0
16	9727.5
17	9918.0
18	11556.5
19	15973.0
20	21752.5
21	26451.5
22	34347.0
23	46565.5
24	60772.5
25	73760.5
26	99573.5
27	141805.5
28	184708.5
29	222448.0
30	278637.0
31	347189.0
32	405254.5
33	451322.5
34	522723.5
35	629558.5
36	721347.0
37	769021.0
38	786396.0
39	835341.0
40	910201.5
41	965271.5
42	993214.5
43	1044333.0
44	1127661.5
45	1211362.0
46	1256990.0
47	1297738.5
48	1342192.0
49	1361129.0
50	1367375.5
51	1367682.0
52	1376744.5
53	1338143.5
54	1293553.0
55	1236497.5
56	1133910.0
57	1017499.0
58	933661.5
59	852138.0
60	736949.5
61	608402.0
62	512154.0
63	443060.5
64	356120.0
65	285082.0
66	214488.5
67	170719.5
68	139771.0
69	103021.5
70	80521.0
71	69206.0
72	60645.0
73	51203.0
74	41507.0
75	32608.5
76	27282.0
77	21522.0
78	18510.0
79	18575.0
80	15951.5
81	13600.0
82	12384.0
83	10503.0
84	9640.0
85	8187.0
86	7708.5
87	7988.5
88	5472.0
89	2475.5
90	1175.5
91	468.0
92	313.5
93	281.0
94	206.0
95	147.0
96	131.0
97	89.5
98	32.5
99	11.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9107509290336364
2	0.16573247954064604
3	0.1074774324002706
4	0.11459487012269053
5	0.1074774324002706
6	0.10762360410705016
7	0.1074774324002706
8	0.10761985611456863
9	0.1074774324002706
10-11	0.1074774324002706
12-13	0.10796467142286913
14-15	0.10769294196795842
16-17	0.10774166587021827
18-19	0.10748680238147443
20-21	0.1074886763777152
22-23	0.10786347562586789
24-25	0.10934205865983032
26-27	0.11826415476210554
28-29	0.1203892664991314
30-31	0.12031618064574162
32-33	0.12559897604845405
34-35	0.13137650645872803
36-37	4.916642455642971E-4
38-39	0.001373780999265027
40-41	4.0354977396989464E-4
42-43	1.1263767703263699E-5
44-45	0.0
46-47	3.7560534200190396E-5
48-49	6.386822647402606E-4
50-51	5.561935914472457E-4
52-53	2.1241008756182916E-4
54-55	1.5044373002060253E-4
56-57	0.0017685582293751917
58-59	0.002129065894645913
60-61	1.3185597379516981E-5
62-63	0.002854035841374196
64-65	0.0
66-67	0.0011237159302786202
68-69	0.0013385778106111672
70-71	1.8929873790366867E-6
72-73	2.0912622670830508E-5
74-75	6.657025411076362E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	36229.0
36	1044.0
37	1191.0
38	1370.0
39	1559.0
40	1919.0
41	2314.0
42	2504.0
43	2556.0
44	2521.0
45	2643.0
46	2821.0
47	3222.0
48	3506.0
49	3875.0
50	4472.0
51	4987.0
52	5454.0
53	5633.0
54	6232.0
55	6223.0
56	6711.0
57	7204.0
58	7632.0
59	8416.0
60	9196.0
61	10267.0
62	10811.0
63	11426.0
64	12232.0
65	13013.0
66	14209.0
67	14041.0
68	15077.0
69	16173.0
70	17980.0
71	47163.0
72	114437.0
73	496445.0
74	1920794.0
75	6117632.0
76	1.7707816E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.001978553510785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.29686326224453	18.441504370791854
2	14.317718879447892	7.732134762360969
3	5.621774629839195	4.55397113962767
4	2.7562360938638837	2.9769531157969977
5	1.732432876607592	2.33895576897776
6	1.2117437489489071	1.963168723288149
7	0.8513557357928933	1.609180252350167
8	0.6443090935769012	1.391809626127637
9	0.5132715527493238	1.247341271351798
>10	3.360016125679194	17.553154727808668
>50	0.3695112124499317	6.950078971093612
>100	0.2753202668108918	14.847247737459245
>500	0.03129543392927621	5.868794815601068
>1k	0.017373005554642534	8.121201284388741
>5k	4.844664652681757E-4	0.7695956973849988
>10k+	2.936160395562757E-4	3.6349077355903856
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	230651	0.864478213856703	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	99458	0.37276783622772053	No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	86620	0.32465110874987585	No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74061	0.2775800711743772	No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70482	0.2641660060829918	No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63954	0.239699111163583	No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55006	0.20616207443887868	No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33896	0.12704195315384198	No Hit
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30135	0.11294575343081861	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	28676	0.1074774324002706	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.747992481527082E-6	0.0
2	0.0	3.747992481527082E-6	0.0	3.747992481527082E-6	1.1243977444581246E-5
3	3.747992481527082E-6	3.747992481527082E-6	0.0	3.747992481527082E-6	1.8739962407635412E-5
4	1.1243977444581246E-5	3.747992481527082E-6	0.0	3.747992481527082E-6	2.2487954889162492E-5
5	1.1243977444581246E-5	3.747992481527082E-6	0.0	3.747992481527082E-6	2.2487954889162492E-5
6	1.8739962407635412E-5	3.747992481527082E-6	0.0	3.747992481527082E-6	2.6235947370689576E-5
7	1.8739962407635412E-5	3.747992481527082E-6	0.0	3.747992481527082E-6	2.6235947370689576E-5
8	1.8739962407635412E-5	3.747992481527082E-6	0.0	3.747992481527082E-6	4.4975909778324984E-5
9	1.8739962407635412E-5	3.747992481527082E-6	0.0	3.747992481527082E-6	4.8723902259852064E-5
10	2.2487954889162492E-5	3.747992481527082E-6	0.0	3.747992481527082E-6	4.8723902259852064E-5
11	4.1227917296797904E-5	3.747992481527082E-6	0.0	7.495984963054164E-6	4.8723902259852064E-5
12	4.8723902259852064E-5	3.747992481527082E-6	0.0	7.495984963054164E-6	5.996787970443331E-5
13	4.8723902259852064E-5	3.747992481527082E-6	0.0	7.495984963054164E-6	6.37158721859604E-5
14	5.247189474137915E-5	3.747992481527082E-6	0.0	1.8739962407635412E-5	6.37158721859604E-5
15	5.247189474137915E-5	3.747992481527082E-6	0.0	2.9983939852216656E-5	6.37158721859604E-5
16	5.621988722290623E-5	3.747992481527082E-6	0.0	2.9983939852216656E-5	6.37158721859604E-5
17	6.746386466748747E-5	3.747992481527082E-6	0.0	2.9983939852216656E-5	6.37158721859604E-5
18	7.495984963054165E-5	3.747992481527082E-6	0.0	2.9983939852216656E-5	6.746386466748747E-5
19	8.620382707512288E-5	3.747992481527082E-6	0.0	2.9983939852216656E-5	6.746386466748747E-5
20	8.995181955664997E-5	3.747992481527082E-6	0.0	2.9983939852216656E-5	6.746386466748747E-5
21	9.369981203817706E-5	3.747992481527082E-6	0.0	2.9983939852216656E-5	6.746386466748747E-5
22	9.369981203817706E-5	3.747992481527082E-6	0.0	2.9983939852216656E-5	6.746386466748747E-5
23	9.744780451970413E-5	3.747992481527082E-6	1.1243977444581246E-5	3.3731932333743736E-5	6.746386466748747E-5
24	1.049437894827583E-4	3.747992481527082E-6	1.1243977444581246E-5	3.3731932333743736E-5	6.746386466748747E-5
25	1.0869178196428538E-4	3.747992481527082E-6	1.1243977444581246E-5	4.1227917296797904E-5	6.746386466748747E-5
26	1.274317443719208E-4	7.495984963054164E-6	1.1243977444581246E-5	4.1227917296797904E-5	6.746386466748747E-5
27	1.274317443719208E-4	7.495984963054164E-6	1.1243977444581246E-5	4.1227917296797904E-5	6.746386466748747E-5
28	1.3117973685344787E-4	7.495984963054164E-6	1.1243977444581246E-5	4.4975909778324984E-5	6.746386466748747E-5
29	1.4242371429802912E-4	7.495984963054164E-6	1.1243977444581246E-5	5.247189474137915E-5	7.121185714901456E-5
30	1.461717067795562E-4	7.495984963054164E-6	1.1243977444581246E-5	5.247189474137915E-5	7.495984963054165E-5
31	1.6491166918719162E-4	7.495984963054164E-6	1.1243977444581246E-5	5.247189474137915E-5	7.495984963054165E-5
32	1.6491166918719162E-4	7.495984963054164E-6	1.1243977444581246E-5	5.247189474137915E-5	7.495984963054165E-5
33	1.9489560903940826E-4	7.495984963054164E-6	1.1243977444581246E-5	5.247189474137915E-5	7.870784211206872E-5
34	1.9864360152093536E-4	7.495984963054164E-6	1.1243977444581246E-5	5.247189474137915E-5	7.870784211206872E-5
35	1.9864360152093536E-4	7.495984963054164E-6	1.1243977444581246E-5	5.247189474137915E-5	8.620382707512288E-5
36	1.9864360152093536E-4	7.495984963054164E-6	1.1243977444581246E-5	5.247189474137915E-5	8.620382707512288E-5
37	1.9864360152093536E-4	7.495984963054164E-6	1.1243977444581246E-5	5.247189474137915E-5	9.744780451970413E-5
38	2.0239159400246243E-4	7.495984963054164E-6	1.1243977444581246E-5	5.621988722290623E-5	1.1243977444581246E-4
39	2.1363557144704368E-4	7.495984963054164E-6	1.1243977444581246E-5	5.621988722290623E-5	1.1993575940886662E-4
40	2.1738356392857075E-4	7.495984963054164E-6	1.1243977444581246E-5	5.621988722290623E-5	1.274317443719208E-4
41	2.2113155641009785E-4	7.495984963054164E-6	1.1243977444581246E-5	5.996787970443331E-5	1.3117973685344787E-4
42	2.3987151881773325E-4	1.4991969926108328E-5	1.1243977444581246E-5	6.746386466748747E-5	1.4242371429802912E-4
43	2.548634887438416E-4	1.4991969926108328E-5	1.1243977444581246E-5	6.746386466748747E-5	1.5741568422413744E-4
44	2.6235947370689574E-4	1.8739962407635412E-5	1.1243977444581246E-5	6.746386466748747E-5	1.5741568422413744E-4
45	2.6610746618842285E-4	1.8739962407635412E-5	1.1243977444581246E-5	7.121185714901456E-5	1.6116367670566454E-4
46	3.0358739100369363E-4	1.8739962407635412E-5	1.1243977444581246E-5	7.121185714901456E-5	1.6116367670566454E-4
47	3.0733538348522074E-4	1.8739962407635412E-5	1.1243977444581246E-5	7.870784211206872E-5	1.6116367670566454E-4
48	3.223273534113291E-4	1.8739962407635412E-5	1.1243977444581246E-5	7.870784211206872E-5	1.6116367670566454E-4
49	3.373193233374374E-4	1.8739962407635412E-5	1.1243977444581246E-5	7.870784211206872E-5	1.6116367670566454E-4
50	3.373193233374374E-4	1.8739962407635412E-5	1.1243977444581246E-5	8.245583459359581E-5	1.7990363911329994E-4
51	3.373193233374374E-4	1.8739962407635412E-5	1.1243977444581246E-5	8.245583459359581E-5	1.873996240763541E-4
52	3.485633007820186E-4	1.8739962407635412E-5	1.1243977444581246E-5	8.245583459359581E-5	1.873996240763541E-4
53	3.710512556711811E-4	1.8739962407635412E-5	1.1243977444581246E-5	8.245583459359581E-5	1.873996240763541E-4
54	3.710512556711811E-4	2.2487954889162492E-5	1.1243977444581246E-5	8.245583459359581E-5	1.873996240763541E-4
55	3.710512556711811E-4	2.2487954889162492E-5	1.1243977444581246E-5	8.995181955664997E-5	1.873996240763541E-4
56	3.710512556711811E-4	2.2487954889162492E-5	1.1243977444581246E-5	9.369981203817706E-5	1.873996240763541E-4
57	3.8229523311576237E-4	2.9983939852216656E-5	1.1243977444581246E-5	9.369981203817706E-5	1.873996240763541E-4
58	4.0103519552339776E-4	2.9983939852216656E-5	1.1243977444581246E-5	1.049437894827583E-4	1.873996240763541E-4
59	4.160271654495061E-4	2.9983939852216656E-5	1.1243977444581246E-5	1.1243977444581246E-4	1.873996240763541E-4
60	4.2727114289408736E-4	2.9983939852216656E-5	1.1243977444581246E-5	1.1993575940886662E-4	1.873996240763541E-4
61	4.347671278571415E-4	2.9983939852216656E-5	1.1243977444581246E-5	1.1993575940886662E-4	1.873996240763541E-4
62	4.347671278571415E-4	2.9983939852216656E-5	1.1243977444581246E-5	1.1993575940886662E-4	1.873996240763541E-4
63	4.347671278571415E-4	2.9983939852216656E-5	1.1243977444581246E-5	1.236837518903937E-4	1.873996240763541E-4
64	4.347671278571415E-4	2.9983939852216656E-5	1.1243977444581246E-5	1.236837518903937E-4	1.873996240763541E-4
65	4.385151203386686E-4	3.3731932333743736E-5	1.4991969926108328E-5	1.274317443719208E-4	1.9489560903940826E-4
>>END_MODULE
