##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543827_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24693983
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.642841011107848	32.0	32.0	32.0	32.0	32.0
2	31.002512271916604	32.0	32.0	32.0	32.0	32.0
3	33.59906868810916	37.0	32.0	37.0	27.0	37.0
4	34.966804504562916	37.0	37.0	37.0	27.0	37.0
5	35.52430258010625	37.0	37.0	37.0	32.0	37.0
6	38.58799765108772	41.0	41.0	41.0	32.0	41.0
7	38.63687340353316	41.0	41.0	41.0	32.0	41.0
8	38.60452050201865	41.0	41.0	41.0	32.0	41.0
9	38.68376029091783	41.0	41.0	41.0	32.0	41.0
10-11	38.74890010655632	41.0	41.0	41.0	37.0	41.0
12-13	38.795130882693165	41.0	41.0	41.0	37.0	41.0
14-15	38.70361788132761	41.0	41.0	41.0	32.0	41.0
16-17	38.55503555258785	41.0	41.0	41.0	32.0	41.0
18-19	38.68082860508975	41.0	41.0	41.0	34.5	41.0
20-21	38.755657947120156	41.0	41.0	41.0	34.5	41.0
22-23	38.532607295469504	41.0	41.0	41.0	32.0	41.0
24-25	38.555425303402856	41.0	41.0	41.0	32.0	41.0
26-27	38.725446660427366	41.0	41.0	41.0	34.5	41.0
28-29	38.431507707768326	41.0	39.0	41.0	32.0	41.0
30-31	38.60982402069362	41.0	41.0	41.0	32.0	41.0
32-33	38.76606372086674	41.0	41.0	41.0	34.5	41.0
34-35	38.68501760125128	41.0	41.0	41.0	34.5	41.0
36-37	38.48407111775528	41.0	41.0	41.0	32.0	41.0
38-39	38.42911429446575	41.0	41.0	41.0	32.0	41.0
40-41	38.45092895961467	41.0	41.0	41.0	32.0	41.0
42-43	38.41666647895439	41.0	41.0	41.0	32.0	41.0
44-45	38.370122186619454	41.0	39.0	41.0	32.0	41.0
46-47	38.38159285852369	41.0	37.0	41.0	32.0	41.0
48-49	38.36270142855415	41.0	37.0	41.0	32.0	41.0
50-51	38.30922241979231	41.0	37.0	41.0	32.0	41.0
52-53	38.34167506646903	41.0	37.0	41.0	32.0	41.0
54-55	38.347913075837894	41.0	37.0	41.0	32.0	41.0
56-57	38.340903373150724	41.0	37.0	41.0	32.0	41.0
58-59	38.35536582623831	41.0	37.0	41.0	32.0	41.0
60-61	38.32006353209317	41.0	37.0	41.0	32.0	41.0
62-63	38.354851827194544	41.0	37.0	41.0	32.0	41.0
64-65	38.34226077443532	41.0	37.0	41.0	32.0	41.0
66-67	38.26608244105701	41.0	37.0	41.0	32.0	41.0
68-69	38.27706635523705	41.0	37.0	41.0	32.0	41.0
70-71	38.10253120778261	41.0	37.0	41.0	32.0	41.0
72-73	38.215429602770605	41.0	37.0	41.0	32.0	41.0
74-75	38.242968058751444	41.0	37.0	41.0	32.0	41.0
76	37.338642159420615	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	14808.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	65.0
13	271.0
14	1173.0
15	4244.0
16	12385.0
17	26438.0
18	42765.0
19	57375.0
20	67517.0
21	77018.0
22	87606.0
23	98906.0
24	111914.0
25	127269.0
26	144160.0
27	164651.0
28	189378.0
29	219434.0
30	256107.0
31	299748.0
32	356143.0
33	425732.0
34	523899.0
35	670370.0
36	903341.0
37	1329806.0
38	2349570.0
39	6084128.0
40	1.0047761E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.66527814221989	19.026002033586987	14.293951718709	31.014768105484126
2	22.31452908581188	27.04904709279839	28.97608265654197	21.66034116484776
3	21.697556745717797	28.077607942729045	27.75061159864542	22.474223712907744
4	25.469798315061848	32.48855053760931	19.396485773589152	22.645165373739694
5	24.732307756143946	34.54194144334228	20.134516948054355	20.591233852459425
6	20.126625035060165	36.69469913010273	20.996546619422194	22.182129215414918
7	20.282464736581325	21.168270370786377	33.789058732897885	24.76020615973441
8	21.19948327931241	23.514968975282745	26.187353390837693	29.098194354567152
9	22.068027806294406	23.284001088393033	27.54925373557682	27.09871736973574
10-11	23.911437375455684	30.52527605897265	21.39004121971723	24.17324534585444
12-13	23.685895093008856	24.816541538130394	25.38027232373749	26.11729104512326
14-15	22.803164536868632	27.73399831269921	25.94975096601627	23.513086184415886
16-17	23.13940895011178	27.698514263096964	25.32568062507729	23.836396161713967
18-19	22.907467690966325	27.970938609913443	25.50161150848779	23.61998219063244
20-21	22.964547944013177	27.927988544374323	25.52948413904862	23.577979372563885
22-23	23.2365243250285	27.56112331108973	25.44987936101962	23.75247300286215
24-25	23.096011552272838	27.487289480919685	25.593061990538303	23.82363697626917
26-27	22.824941000567247	27.877331508117265	25.53109721267227	23.76663027864322
28-29	23.46913243474464	27.2500558873292	25.367344329955145	23.913467347971014
30-31	23.105207881812408	27.499452830348876	25.621714107185596	23.77362518065312
32-33	22.926561866181665	27.745147758404947	25.699082875511476	23.629207499901913
34-35	23.17275538934271	27.624830139721958	25.269597105456214	23.932817365479124
36-37	22.90059440232562	27.389183724716375	25.72846291915929	23.981758953798717
38-39	22.655150256940424	27.699593649865317	25.870476740988963	23.774779352205297
40-41	23.138174206247598	27.383382201450623	25.474690949136956	24.003752643164823
42-43	22.753533251471588	27.402879634251082	25.73565795379182	24.107929160485508
44-45	22.873640676022102	27.2951197796432	25.776903568865063	24.054335975469638
46-47	23.0964964746639	27.197975211138054	25.501819031461682	24.203709282736366
48-49	22.946201527795747	27.148886293033787	25.822671039623728	24.08224113954674
50-51	22.80588018073761	27.268666254574374	25.871614899002694	24.05383866568532
52-53	23.014751883771147	27.207087867681366	25.495563682449863	24.28259656609762
54-55	22.93513866599936	26.95488427685917	25.94862836090594	24.161348696235528
56-57	22.728942877249754	27.34650477481842	25.719681051397856	24.20487129653397
58-59	22.904486678773175	27.403389758465817	25.66210701729954	24.030016545461464
60-61	22.8652324879307	27.116450724418662	25.968342006593392	24.049974781057244
62-63	22.743385704208126	27.45532890023406	25.81363303941901	23.987652356138806
64-65	23.14171816369769	27.188384990399523	25.671320995601643	23.998575850301144
66-67	22.997525110147826	27.13416584521198	25.964872873968652	23.903436170671544
68-69	22.810344156113526	27.218439227787112	26.104565554885912	23.866651061213453
70-71	23.14222929082791	27.312030283998983	25.760829192865987	23.784911232307117
72-73	22.840305322680784	27.147628394579225	25.882289766033175	24.129776516706816
74-75	22.79999994720826	23.913175361972062	27.591272628024257	25.695552062795425
76	24.068307493487588	0.0	38.641175960009825	37.29051654650258
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	45198.0
1	27980.0
2	10257.5
3	9389.5
4	9025.5
5	8227.5
6	8529.0
7	11421.5
8	13224.0
9	14285.5
10	18494.5
11	41914.5
12	62186.5
13	80400.5
14	73431.0
15	39412.5
16	23395.5
17	16957.5
18	17609.0
19	19825.0
20	25627.5
21	31423.5
22	39345.5
23	53303.5
24	73007.0
25	91197.0
26	107919.5
27	137629.5
28	176577.5
29	203520.5
30	251570.0
31	326992.0
32	393681.0
33	437687.5
34	517373.5
35	622700.5
36	689030.0
37	724244.0
38	745741.5
39	801728.0
40	873134.5
41	925222.5
42	953831.5
43	1017687.0
44	1122519.5
45	1197134.5
46	1238372.5
47	1315340.5
48	1373715.5
49	1361583.5
50	1342058.0
51	1322996.0
52	1310726.0
53	1278712.5
54	1234200.0
55	1147914.5
56	1019902.0
57	883822.0
58	790909.0
59	711629.0
60	603281.5
61	504637.0
62	432365.5
63	363820.5
64	286293.5
65	225145.5
66	169494.5
67	141216.5
68	121905.5
69	97063.0
70	75677.5
71	62581.5
72	56758.5
73	53567.0
74	43207.0
75	31430.5
76	25360.0
77	19488.0
78	16391.0
79	14719.0
80	13946.0
81	12675.0
82	11152.5
83	10221.0
84	9155.0
85	7222.0
86	5517.0
87	4870.0
88	4599.5
89	2952.0
90	1201.0
91	699.0
92	581.0
93	494.5
94	396.5
95	333.0
96	280.0
97	251.5
98	199.5
99	173.5
100	173.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.2293804527200005
2	0.1975015533136149
3	0.05996602492194151
4	0.06112825136390513
5	0.06259419551718327
6	0.06065040216477026
7	0.06020494952150894
8	0.06184907473209161
9	0.06235527091761584
10-11	0.06081238494413801
12-13	0.0600429667421412
14-15	0.0604256510583975
16-17	0.0609500703066006
18-19	0.06235324613287375
20-21	0.06116469748926287
22-23	0.060654451734254454
24-25	0.06717425860380645
26-27	0.06475869040648485
28-29	0.06925371253393994
30-31	0.07806557573154561
32-33	0.08944284119738805
34-35	0.1004718436875898
36-37	0.00288440066056221
38-39	0.004086973222905588
40-41	0.0011111489604674286
42-43	0.002216760927343501
44-45	0.0014931188825108774
46-47	1.7248899200619043E-4
48-49	0.0041939197015001
50-51	0.00396005591111555
52-53	0.001548226807912902
54-55	0.0099594014625378
56-57	0.0040745116917431535
58-59	0.0041444890194145085
60-61	0.0010695373892241928
62-63	2.8955318416029076E-4
64-65	6.389118331268437E-4
66-67	0.00533619009229933
68-69	0.0036549642528738383
70-71	0.0037424005290024563
72-73	0.0014232985151124127
74-75	2.8155515051696387E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	26094.0
36	1439.0
37	1725.0
38	1992.0
39	2190.0
40	2766.0
41	3027.0
42	3326.0
43	3327.0
44	3401.0
45	3482.0
46	3921.0
47	4100.0
48	4594.0
49	5072.0
50	5325.0
51	6091.0
52	6627.0
53	7019.0
54	7227.0
55	7646.0
56	7919.0
57	8428.0
58	9013.0
59	9679.0
60	10463.0
61	11653.0
62	11797.0
63	12958.0
64	13819.0
65	14833.0
66	16019.0
67	16194.0
68	16587.0
69	17846.0
70	20556.0
71	44358.0
72	133743.0
73	576397.0
74	1800874.0
75	6069784.0
76	1.5760672E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.847128529226715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.97267053001762	23.12691604018822
2	11.15222395417778	6.880281714024859
3	4.355797774865133	4.030915614257535
4	2.2425294497840578	2.767023766722596
5	1.4123338878968335	2.1783222483068045
6	0.9534802878173363	1.764727739303127
7	0.6927372126672955	1.4958267685288427
8	0.5111070630223389	1.261294821219653
9	0.41049922234493075	1.1396450045539526
>10	2.6911605248321195	16.187136515549184
>50	0.31005029085055547	6.7025475799971685
>100	0.248158143046405	15.553389629643776
>500	0.029900200967831147	6.375676131374404
>1k	0.016867933975736632	8.860423992774482
>5k	4.0063395130555686E-4	0.812066661733722
>10k+	8.288978302872481E-5	0.8638057718214224
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	88417	0.35805078508396154	No Hit
CCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36227	0.1467037537038881	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	4.049569484193781E-6
5	0.0	0.0	0.0	0.0	4.049569484193781E-6
6	0.0	0.0	0.0	0.0	8.099138968387562E-6
7	0.0	0.0	0.0	3.644612535774403E-5	8.099138968387562E-6
8	0.0	0.0	0.0	4.4545264326131595E-5	8.099138968387562E-6
9	4.049569484193781E-6	0.0	0.0	4.859483381032537E-5	8.099138968387562E-6
10	8.099138968387562E-6	0.0	0.0	4.859483381032537E-5	8.099138968387562E-6
11	8.099138968387562E-6	0.0	0.0	4.859483381032537E-5	8.099138968387562E-6
12	8.099138968387562E-6	0.0	0.0	4.859483381032537E-5	8.099138968387562E-6
13	1.2148708452581343E-5	0.0	0.0	4.859483381032537E-5	8.099138968387562E-6
14	3.644612535774403E-5	0.0	0.0	4.859483381032537E-5	8.099138968387562E-6
15	3.644612535774403E-5	0.0	0.0	4.859483381032537E-5	8.099138968387562E-6
16	5.6693972778712936E-5	0.0	0.0	4.859483381032537E-5	8.099138968387562E-6
17	6.884268123129428E-5	0.0	0.0	4.859483381032537E-5	8.099138968387562E-6
18	7.289225071548806E-5	0.0	0.0	4.859483381032537E-5	8.099138968387562E-6
19	7.694182019968185E-5	0.0	0.0	4.859483381032537E-5	8.099138968387562E-6
20	7.694182019968185E-5	0.0	0.0	4.859483381032537E-5	8.099138968387562E-6
21	8.099138968387562E-5	4.049569484193781E-6	0.0	4.859483381032537E-5	8.099138968387562E-6
22	8.909052865226319E-5	4.049569484193781E-6	0.0	5.264440329451915E-5	8.099138968387562E-6
23	1.0123923710484453E-4	4.049569484193781E-6	0.0	5.6693972778712936E-5	8.099138968387562E-6
24	1.1338794555742587E-4	4.049569484193781E-6	0.0	5.6693972778712936E-5	8.099138968387562E-6
25	1.2553665401000721E-4	4.049569484193781E-6	0.0	5.6693972778712936E-5	8.099138968387562E-6
26	1.3363579297839478E-4	4.049569484193781E-6	0.0	5.6693972778712936E-5	8.099138968387562E-6
27	1.3363579297839478E-4	4.049569484193781E-6	0.0	6.0743542262906715E-5	8.099138968387562E-6
28	1.4173493194678233E-4	4.049569484193781E-6	0.0	6.0743542262906715E-5	8.099138968387562E-6
29	1.5793320988355747E-4	4.049569484193781E-6	0.0	6.0743542262906715E-5	8.099138968387562E-6
30	1.8628019627291392E-4	4.049569484193781E-6	0.0	6.0743542262906715E-5	8.099138968387562E-6
31	2.0652804369388283E-4	4.049569484193781E-6	0.0	6.479311174710049E-5	8.099138968387562E-6
32	2.3487503008323932E-4	4.049569484193781E-6	0.0	6.479311174710049E-5	8.099138968387562E-6
33	2.5917244698840197E-4	4.049569484193781E-6	0.0	6.479311174710049E-5	8.099138968387562E-6
34	2.713211554409833E-4	4.049569484193781E-6	0.0	7.289225071548806E-5	8.099138968387562E-6
35	2.95618572346146E-4	4.049569484193781E-6	0.0	7.289225071548806E-5	8.099138968387562E-6
36	3.077672807987274E-4	4.049569484193781E-6	0.0	9.314009813645696E-5	8.099138968387562E-6
37	3.442134061564714E-4	4.049569484193781E-6	0.0	1.052888065890383E-4	8.099138968387562E-6
38	3.7256039254582784E-4	4.049569484193781E-6	0.0	1.052888065890383E-4	8.099138968387562E-6
39	4.049569484193781E-4	4.049569484193781E-6	0.0	1.1743751504161966E-4	8.099138968387562E-6
40	4.25204795840347E-4	4.049569484193781E-6	0.0	1.2148708452581343E-4	8.099138968387562E-6
41	4.495022127455097E-4	4.049569484193781E-6	0.0	1.2553665401000721E-4	8.099138968387562E-6
42	4.6570049068228483E-4	4.049569484193781E-6	0.0	1.2958622349420099E-4	8.099138968387562E-6
43	4.899979075874475E-4	4.049569484193781E-6	0.0	1.4173493194678233E-4	8.099138968387562E-6
44	5.142953244926102E-4	4.049569484193781E-6	0.0	1.498340709151699E-4	8.099138968387562E-6
45	5.264440329451915E-4	4.049569484193781E-6	0.0	1.5793320988355747E-4	8.099138968387562E-6
46	5.426423108819666E-4	4.049569484193781E-6	0.0	1.7413148782033258E-4	8.099138968387562E-6
47	5.790884362397107E-4	4.049569484193781E-6	0.0	1.8628019627291392E-4	8.099138968387562E-6
48	5.952867141764858E-4	4.049569484193781E-6	0.0	2.0652804369388283E-4	8.099138968387562E-6
49	6.236337005658423E-4	4.049569484193781E-6	0.0	2.105776131780766E-4	8.099138968387562E-6
50	6.438815479868112E-4	4.049569484193781E-6	0.0	2.146271826622704E-4	8.099138968387562E-6
51	6.600798259235863E-4	4.049569484193781E-6	0.0	2.1867675214646418E-4	8.099138968387562E-6
52	6.884268123129428E-4	4.049569484193781E-6	0.0	2.2272632163065797E-4	8.099138968387562E-6
53	7.127242292181055E-4	4.049569484193781E-6	0.0	2.2272632163065797E-4	8.099138968387562E-6
54	7.248729376706869E-4	4.049569484193781E-6	0.0	2.2677589111485175E-4	8.099138968387562E-6
55	7.329720766390743E-4	4.049569484193781E-6	0.0	2.3082546059904552E-4	8.099138968387562E-6
56	7.694182019968184E-4	4.049569484193781E-6	0.0	2.3082546059904552E-4	8.099138968387562E-6
57	7.896660494177873E-4	4.049569484193781E-6	0.0	2.5107330802001443E-4	8.099138968387562E-6
58	8.909052865226319E-4	4.049569484193781E-6	0.0	2.5107330802001443E-4	8.099138968387562E-6
59	0.0010042932320800577	4.049569484193781E-6	0.0	2.5107330802001443E-4	8.099138968387562E-6
60	0.001052888065890383	8.099138968387562E-6	0.0	2.5107330802001443E-4	8.099138968387562E-6
61	0.0010852846217639333	1.2148708452581343E-5	0.0	2.5917244698840197E-4	8.099138968387562E-6
62	0.001129829886090065	1.2148708452581343E-5	0.0	2.6727158595678957E-4	8.099138968387562E-6
63	0.001129829886090065	1.2148708452581343E-5	0.0	2.753707249251771E-4	8.099138968387562E-6
64	0.001129829886090065	1.2148708452581343E-5	0.0	2.8346986389356465E-4	8.099138968387562E-6
65	0.0011338794555742586	1.6198277936775123E-5	0.0	2.8751943337775845E-4	1.2148708452581343E-5
>>END_MODULE
