##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543827_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24693983
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.447575144115067	32.0	32.0	32.0	32.0	32.0
2	31.749457185582415	32.0	32.0	32.0	32.0	32.0
3	35.85897426915698	37.0	37.0	37.0	32.0	37.0
4	36.42826274724494	37.0	37.0	37.0	37.0	37.0
5	36.59232109295613	37.0	37.0	37.0	37.0	37.0
6	40.147177188872284	41.0	41.0	41.0	37.0	41.0
7	40.20867771715887	41.0	41.0	41.0	37.0	41.0
8	40.17655252293646	41.0	41.0	41.0	37.0	41.0
9	40.15768776547712	41.0	41.0	41.0	37.0	41.0
10-11	39.93897181349805	41.0	41.0	41.0	37.0	41.0
12-13	39.87262222947185	41.0	41.0	41.0	37.0	41.0
14-15	39.799580630633784	41.0	41.0	41.0	37.0	41.0
16-17	39.7773168265322	41.0	41.0	41.0	37.0	41.0
18-19	39.728931173233576	41.0	41.0	41.0	37.0	41.0
20-21	39.695278278923254	41.0	41.0	41.0	37.0	41.0
22-23	39.67375736834353	41.0	41.0	41.0	37.0	41.0
24-25	39.6376479646884	41.0	41.0	41.0	37.0	41.0
26-27	39.51889093792605	41.0	41.0	41.0	37.0	41.0
28-29	39.52521342952248	41.0	41.0	41.0	37.0	41.0
30-31	39.563174863285525	41.0	41.0	41.0	37.0	41.0
32-33	39.623436041079316	41.0	41.0	41.0	37.0	41.0
34-35	39.62864089199381	41.0	41.0	41.0	37.0	41.0
36-37	39.768108740753064	41.0	41.0	41.0	37.0	41.0
38-39	39.84909647957063	41.0	41.0	41.0	37.0	41.0
40-41	39.91838078550933	41.0	41.0	41.0	37.0	41.0
42-43	39.93501928405584	41.0	41.0	41.0	37.0	41.0
44-45	39.94632381932434	41.0	41.0	41.0	37.0	41.0
46-47	39.86372278330555	41.0	41.0	41.0	37.0	41.0
48-49	39.935637185287845	41.0	41.0	41.0	37.0	41.0
50-51	39.9240447745822	41.0	41.0	41.0	37.0	41.0
52-53	39.86828371538291	41.0	41.0	41.0	37.0	41.0
54-55	39.90590168562417	41.0	41.0	41.0	37.0	41.0
56-57	39.905865393190766	41.0	41.0	41.0	37.0	41.0
58-59	39.834877727803075	41.0	41.0	41.0	37.0	41.0
60-61	39.87044634544401	41.0	41.0	41.0	37.0	41.0
62-63	39.85290854493667	41.0	41.0	41.0	37.0	41.0
64-65	39.79224088015354	41.0	41.0	41.0	37.0	41.0
66-67	39.79100159800686	41.0	41.0	41.0	37.0	41.0
68-69	39.78208315934711	41.0	41.0	41.0	37.0	41.0
70-71	39.76431388060712	41.0	41.0	41.0	37.0	41.0
72-73	39.71217724354241	41.0	41.0	41.0	37.0	41.0
74-75	39.692096748516946	41.0	41.0	41.0	37.0	41.0
76	39.21668019488258	41.0	41.0	41.0	37.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	15316.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	3.0
15	6.0
16	11.0
17	27.0
18	62.0
19	188.0
20	384.0
21	890.0
22	1964.0
23	3900.0
24	6659.0
25	11890.0
26	19857.0
27	31699.0
28	48917.0
29	72989.0
30	103393.0
31	143662.0
32	194382.0
33	259071.0
34	342296.0
35	454751.0
36	618650.0
37	890195.0
38	1497373.0
39	4260394.0
40	1.5715053E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.903650174025024	10.14980101794283	12.181000083925571	35.76554872410657
2	23.145786355769253	14.69751611400725	33.51003168309638	28.64666584712711
3	22.73905231591317	19.43642255880352	27.306470807357623	30.51805431792568
4	27.589790318887097	26.360387201723007	20.65322105699621	25.39660142239369
5	26.89145649560408	29.468629727853617	24.120954344900394	19.51895943164191
6	22.30903460864163	31.938992247301854	26.368975689659322	19.38299745439719
7	18.94351911308662	22.09116075839915	41.62023418849973	17.345085940014506
8	21.59768985340218	22.036223519423118	32.98439008328145	23.38169654389326
9	21.69680801641353	21.47324245673399	34.62330441105267	22.206645115799812
10-11	24.574886885097968	29.361083400493225	25.65619123593669	20.407838478472115
12-13	24.25832241005438	23.505900171215398	29.668525884823076	22.56725153390715
14-15	23.295644418208497	25.453365301099424	29.777827024205454	21.473163256486618
16-17	23.700839893741126	25.686459174058427	29.086099218440204	21.526601713760236
18-19	23.704229949229426	25.950956018082827	28.863761471700357	21.481052560987397
20-21	23.329826998127352	25.89345245539735	29.19564505590021	21.58107549057509
22-23	23.75648593398128	25.614915298439627	28.738115591747558	21.89048317583153
24-25	23.679305505879604	25.729593917722195	28.65923671300397	21.931863863394234
26-27	23.4036470444631	26.078879293645414	28.67199618474114	21.84547747715035
28-29	23.82868823538423	25.802089234286505	28.621553684614266	21.747668845714998
30-31	23.537391120312982	25.912661833272733	28.50936026033755	22.040586786076734
32-33	23.49309808739661	25.711152513453467	28.645357079395218	22.150392319754705
34-35	23.550329917866865	25.878264761026287	28.5647247769225	22.00668054418435
36-37	24.025013862299705	25.546543526404857	28.29602108670903	22.132421524586416
38-39	23.492615437855914	26.019267847735733	28.57803097196896	21.91008574243939
40-41	23.875089407600825	25.53726022161426	28.622961240569435	21.964689130215486
42-43	23.49133619449681	25.262985202394784	29.040845810157567	22.204832792950835
44-45	23.67203275074638	25.444413959787354	28.942337662506283	21.94121562695998
46-47	23.658300396339737	25.6013594588951	28.78711436220675	21.953225782558413
48-49	23.697590183369996	25.45419349277872	28.643007145390413	22.205209178460873
50-51	23.45785361151115	25.748770172076906	28.646262731474547	22.147113484937396
52-53	23.697195234928362	25.505564936531517	28.524874287381973	22.27236554115815
54-55	23.560819324113673	25.69306721965592	28.30389916216078	22.442214294069622
56-57	23.802755095572834	25.446252429974702	28.629013786055797	22.121978688396666
58-59	23.790439932719373	25.38281280836521	28.535418094444765	22.291329164470653
60-61	23.307305017169348	25.473255430985127	28.744282544968307	22.475157006877215
62-63	23.265210915535622	25.675330730768557	28.895114556909085	22.164343796786735
64-65	23.67932792551274	25.647312026196868	28.504866393507744	22.16849365478265
66-67	23.7539680122979	25.486360337718132	28.432557227969546	22.327114422014418
68-69	23.43564221501379	25.869599915528717	28.509646669400524	22.18511120005697
70-71	23.751824757087732	25.65960322040885	28.3477391701297	22.240832852373718
72-73	23.683654191386943	25.305320888450694	28.52592233987793	22.485102580284433
74-75	23.79871768604343	22.186284649658386	30.112890615395965	23.90210704890222
76	24.795777449869487	0.0	41.622417806785585	33.581804743344925
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	278665.0
1	191501.0
2	67306.5
3	27684.0
4	25090.0
5	28545.5
6	39308.5
7	54394.5
8	62171.5
9	61152.5
10	69261.5
11	68944.0
12	59507.0
13	47650.0
14	23472.0
15	9647.0
16	7951.0
17	8066.5
18	9469.5
19	13455.0
20	18811.5
21	23349.5
22	30466.0
23	41314.5
24	54010.5
25	66395.5
26	91951.5
27	135203.0
28	180529.0
29	219485.0
30	271038.0
31	327547.5
32	383720.5
33	437902.0
34	508919.5
35	610638.0
36	704312.0
37	751802.0
38	770377.0
39	820348.5
40	890256.0
41	938434.0
42	964250.0
43	1024829.0
44	1108475.5
45	1178317.0
46	1215220.5
47	1272259.5
48	1322151.0
49	1310625.5
50	1294096.0
51	1289524.0
52	1300140.0
53	1250805.5
54	1194176.5
55	1130383.5
56	1013146.0
57	887806.5
58	800916.0
59	717988.0
60	607461.5
61	495790.0
62	413000.0
63	351530.5
64	276904.5
65	219045.0
66	164130.0
67	131350.5
68	105154.0
69	75719.5
70	58975.0
71	50118.5
72	43233.0
73	36247.0
74	28741.5
75	21576.0
76	18801.5
77	15029.0
78	12161.0
79	11863.5
80	10182.5
81	8991.0
82	8106.0
83	6519.0
84	5612.5
85	4736.0
86	4403.0
87	4164.0
88	2760.5
89	1084.5
90	389.0
91	132.0
92	90.5
93	71.5
94	46.0
95	34.5
96	29.5
97	19.5
98	8.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8905691722554439
2	0.12038155205662854
3	0.062023206219911954
4	0.06936912526423947
5	0.062023206219911954
6	0.062156842012890345
7	0.062023206219911954
8	0.06226618038896358
9	0.062023206219911954
10-11	0.062023206219911954
12-13	0.06244233666152601
14-15	0.06225808124999519
16-17	0.06230262651432132
18-19	0.06203130535888034
20-21	0.062037379713106626
22-23	0.06269745953903022
24-25	0.06475869040648485
26-27	0.0742326582147562
28-29	0.07814454233648739
30-31	0.08105820758036482
32-33	0.0901879619824797
34-35	0.0995606905536462
36-37	4.905370239203893E-4
38-39	0.0014130217598052762
40-41	3.6295172129752445E-4
42-43	8.112632545204601E-6
44-45	0.0
46-47	1.2175711096025856E-5
48-49	6.536495538303854E-4
50-51	5.381594661945484E-4
52-53	2.0724161531917017E-4
54-55	1.2807437193395292E-4
56-57	0.0016904062973920203
58-59	0.0020518667539714255
60-61	6.111574866466163E-6
62-63	0.0027547988606347663
64-65	0.0
66-67	0.0011464775010125686
68-69	0.0011622851245838691
70-71	2.0492526816059506E-6
72-73	1.8526437236228565E-5
74-75	5.59472579953822E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	26430.0
36	1419.0
37	1695.0
38	1966.0
39	2173.0
40	2746.0
41	2997.0
42	3294.0
43	3302.0
44	3366.0
45	3439.0
46	3876.0
47	4072.0
48	4545.0
49	5056.0
50	5304.0
51	6053.0
52	6586.0
53	6975.0
54	7209.0
55	7637.0
56	7907.0
57	8425.0
58	9025.0
59	9672.0
60	10444.0
61	11653.0
62	11741.0
63	12939.0
64	13790.0
65	14378.0
66	15247.0
67	15729.0
68	16538.0
69	17561.0
70	19311.0
71	45858.0
72	108023.0
73	461293.0
74	1791237.0
75	5724461.0
76	1.6258611E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.161601274771645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.21126678220408	18.368391665526424
2	13.285081903645926	6.951180313317379
3	5.243328782426487	4.11521630875127
4	2.6313106919181495	2.7535720460802446
5	1.6551520553359396	2.1650714060408816
6	1.112190685058494	1.7458013546409266
7	0.7944495498754996	1.454885064973524
8	0.5938489829130261	1.2428832246719412
9	0.4797651921577077	1.1296283096469735
>10	3.2693786042436632	16.704639531550086
>50	0.3772883564788461	6.909360094207721
>100	0.28883780320569236	15.235224659487931
>500	0.03552130971115033	6.401798228436789
>1k	0.021518585643051792	10.323708665625292
>5k	7.669786702376924E-4	1.2415182659575177
>10k+	2.937365120059216E-4	3.2571208610850353
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	243236	0.9850010830573585	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	80729	0.32691769488947975	No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71707	0.29038247900308345	No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56786	0.22995885272942806	No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52196	0.21137132879697862	No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49727	0.20137294174050416	No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41662	0.16871316385048132	No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25208	0.10208154755755683	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.049569484193781E-6	0.0
2	0.0	0.0	0.0	2.4297416905162687E-5	8.099138968387562E-6
3	4.049569484193781E-6	4.049569484193781E-6	0.0	2.4297416905162687E-5	1.2148708452581343E-5
4	1.2148708452581343E-5	4.049569484193781E-6	0.0	3.2396555873550246E-5	1.2148708452581343E-5
5	2.0247847420968905E-5	4.049569484193781E-6	0.0	3.644612535774403E-5	1.2148708452581343E-5
6	3.2396555873550246E-5	4.049569484193781E-6	0.0	3.644612535774403E-5	1.2148708452581343E-5
7	3.2396555873550246E-5	4.049569484193781E-6	0.0	3.644612535774403E-5	1.2148708452581343E-5
8	3.2396555873550246E-5	4.049569484193781E-6	0.0	4.049569484193781E-5	1.6198277936775123E-5
9	3.2396555873550246E-5	4.049569484193781E-6	0.0	4.859483381032537E-5	1.6198277936775123E-5
10	3.644612535774403E-5	4.049569484193781E-6	0.0	4.859483381032537E-5	1.6198277936775123E-5
11	3.644612535774403E-5	4.049569484193781E-6	0.0	5.264440329451915E-5	1.6198277936775123E-5
12	4.4545264326131595E-5	4.049569484193781E-6	0.0	5.264440329451915E-5	1.6198277936775123E-5
13	4.4545264326131595E-5	4.049569484193781E-6	0.0	5.264440329451915E-5	2.4297416905162687E-5
14	4.4545264326131595E-5	8.099138968387562E-6	0.0	5.264440329451915E-5	3.2396555873550246E-5
15	5.264440329451915E-5	8.099138968387562E-6	0.0	5.264440329451915E-5	3.2396555873550246E-5
16	6.0743542262906715E-5	8.099138968387562E-6	0.0	5.264440329451915E-5	3.2396555873550246E-5
17	6.479311174710049E-5	8.099138968387562E-6	0.0	6.479311174710049E-5	3.644612535774403E-5
18	6.479311174710049E-5	8.099138968387562E-6	0.0	6.479311174710049E-5	4.859483381032537E-5
19	6.884268123129428E-5	8.099138968387562E-6	0.0	6.479311174710049E-5	4.859483381032537E-5
20	6.884268123129428E-5	8.099138968387562E-6	0.0	6.479311174710049E-5	4.859483381032537E-5
21	6.884268123129428E-5	8.099138968387562E-6	0.0	6.884268123129428E-5	4.859483381032537E-5
22	8.099138968387562E-5	8.099138968387562E-6	0.0	6.884268123129428E-5	4.859483381032537E-5
23	8.50409591680694E-5	8.099138968387562E-6	0.0	6.884268123129428E-5	4.859483381032537E-5
24	9.718966762065075E-5	1.2148708452581343E-5	0.0	7.694182019968185E-5	4.859483381032537E-5
25	9.718966762065075E-5	1.2148708452581343E-5	0.0	8.099138968387562E-5	4.859483381032537E-5
26	1.0933837607323209E-4	1.2148708452581343E-5	0.0	8.099138968387562E-5	5.264440329451915E-5
27	1.1338794555742587E-4	1.2148708452581343E-5	0.0	8.50409591680694E-5	5.264440329451915E-5
28	1.2148708452581343E-4	1.2148708452581343E-5	0.0	8.909052865226319E-5	5.6693972778712936E-5
29	1.2553665401000721E-4	1.2148708452581343E-5	0.0	8.909052865226319E-5	6.479311174710049E-5
30	1.2553665401000721E-4	1.2148708452581343E-5	0.0	9.314009813645696E-5	6.479311174710049E-5
31	1.2553665401000721E-4	1.2148708452581343E-5	0.0	9.718966762065075E-5	6.479311174710049E-5
32	1.2958622349420099E-4	1.2148708452581343E-5	0.0	9.718966762065075E-5	6.884268123129428E-5
33	1.3768536246258856E-4	1.2148708452581343E-5	0.0	9.718966762065075E-5	6.884268123129428E-5
34	1.4173493194678233E-4	1.2148708452581343E-5	0.0	9.718966762065075E-5	6.884268123129428E-5
35	1.4578450143097613E-4	2.0247847420968905E-5	0.0	9.718966762065075E-5	7.289225071548806E-5
36	1.498340709151699E-4	2.0247847420968905E-5	0.0	1.0123923710484453E-4	8.099138968387562E-5
37	1.538836403993637E-4	2.0247847420968905E-5	0.0	1.0123923710484453E-4	8.50409591680694E-5
38	1.5793320988355747E-4	2.0247847420968905E-5	0.0	1.0123923710484453E-4	1.052888065890383E-4
39	1.5793320988355747E-4	2.0247847420968905E-5	0.0	1.1338794555742587E-4	1.1338794555742587E-4
40	1.5793320988355747E-4	2.0247847420968905E-5	4.049569484193781E-6	1.2148708452581343E-4	1.1338794555742587E-4
41	1.5793320988355747E-4	2.0247847420968905E-5	4.049569484193781E-6	1.2148708452581343E-4	1.2148708452581343E-4
42	1.7413148782033258E-4	2.0247847420968905E-5	4.049569484193781E-6	1.2553665401000721E-4	1.2148708452581343E-4
43	1.7413148782033258E-4	2.4297416905162687E-5	1.2148708452581343E-5	1.2553665401000721E-4	1.2553665401000721E-4
44	1.7818105730452638E-4	2.8346986389356468E-5	1.2148708452581343E-5	1.3363579297839478E-4	1.2553665401000721E-4
45	1.8223062678872015E-4	2.8346986389356468E-5	1.2148708452581343E-5	1.4578450143097613E-4	1.2553665401000721E-4
46	1.9032976575710772E-4	2.8346986389356468E-5	1.2148708452581343E-5	1.4578450143097613E-4	1.2958622349420099E-4
47	1.9032976575710772E-4	2.8346986389356468E-5	1.2148708452581343E-5	1.4578450143097613E-4	1.2958622349420099E-4
48	1.943793352413015E-4	2.8346986389356468E-5	1.2148708452581343E-5	1.538836403993637E-4	1.2958622349420099E-4
49	2.146271826622704E-4	2.8346986389356468E-5	1.2148708452581343E-5	1.5793320988355747E-4	1.2958622349420099E-4
50	2.146271826622704E-4	2.8346986389356468E-5	1.2148708452581343E-5	1.6603234885194504E-4	1.4578450143097613E-4
51	2.1867675214646418E-4	2.8346986389356468E-5	1.2148708452581343E-5	1.700819183361388E-4	1.538836403993637E-4
52	2.2677589111485175E-4	2.8346986389356468E-5	1.2148708452581343E-5	1.8223062678872015E-4	1.538836403993637E-4
53	2.2677589111485175E-4	2.8346986389356468E-5	1.2148708452581343E-5	1.9032976575710772E-4	1.538836403993637E-4
54	2.2677589111485175E-4	2.8346986389356468E-5	1.2148708452581343E-5	2.0247847420968906E-4	1.538836403993637E-4
55	2.2677589111485175E-4	2.8346986389356468E-5	1.2148708452581343E-5	2.105776131780766E-4	1.5793320988355747E-4
56	2.3082546059904552E-4	3.2396555873550246E-5	1.2148708452581343E-5	2.146271826622704E-4	1.5793320988355747E-4
57	2.4297416905162686E-4	3.2396555873550246E-5	1.2148708452581343E-5	2.1867675214646418E-4	1.5793320988355747E-4
58	2.6322201647259577E-4	3.2396555873550246E-5	1.2148708452581343E-5	2.389245995674331E-4	1.5793320988355747E-4
59	2.996681418303398E-4	3.2396555873550246E-5	1.6198277936775123E-5	2.4297416905162686E-4	1.6198277936775124E-4
60	3.1181685028292114E-4	3.2396555873550246E-5	1.6198277936775123E-5	2.4702373853582063E-4	1.6198277936775124E-4
61	3.1586641976711494E-4	3.2396555873550246E-5	1.6198277936775123E-5	2.4702373853582063E-4	1.6198277936775124E-4
62	3.239655587355025E-4	3.2396555873550246E-5	1.6198277936775123E-5	2.5107330802001443E-4	1.6198277936775124E-4
63	3.239655587355025E-4	3.2396555873550246E-5	1.6198277936775123E-5	2.5107330802001443E-4	1.6603234885194504E-4
64	3.239655587355025E-4	3.2396555873550246E-5	1.6198277936775123E-5	2.5512287750420823E-4	1.6603234885194504E-4
65	3.280151282196963E-4	3.644612535774403E-5	2.0247847420968905E-5	2.5917244698840197E-4	1.7413148782033258E-4
>>END_MODULE
