##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543826_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24682746
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.465986483027457	32.0	32.0	32.0	27.0	32.0
2	30.859870372607652	32.0	32.0	32.0	32.0	32.0
3	33.45981042789972	37.0	32.0	37.0	27.0	37.0
4	34.74122964276341	37.0	37.0	37.0	27.0	37.0
5	35.29440958473583	37.0	37.0	37.0	32.0	37.0
6	38.38080442913442	41.0	37.0	41.0	32.0	41.0
7	38.4032243819225	41.0	41.0	41.0	32.0	41.0
8	38.40518364528809	41.0	41.0	41.0	32.0	41.0
9	38.50304463692978	41.0	41.0	41.0	32.0	41.0
10-11	38.50646500190862	41.0	41.0	41.0	32.0	41.0
12-13	38.48639407868152	41.0	41.0	41.0	32.0	41.0
14-15	38.47455285161546	41.0	41.0	41.0	32.0	41.0
16-17	38.34157506219121	41.0	41.0	41.0	32.0	41.0
18-19	38.40780780631134	41.0	41.0	41.0	32.0	41.0
20-21	38.45111273275672	41.0	41.0	41.0	32.0	41.0
22-23	38.35011309114472	41.0	41.0	41.0	32.0	41.0
24-25	38.33969575346276	41.0	41.0	41.0	32.0	41.0
26-27	38.437690644306755	41.0	41.0	41.0	32.0	41.0
28-29	38.285111611973804	41.0	39.0	41.0	32.0	41.0
30-31	38.33978889544947	41.0	41.0	41.0	32.0	41.0
32-33	38.50126197466035	41.0	41.0	41.0	32.0	41.0
34-35	38.4415532817945	41.0	41.0	41.0	32.0	41.0
36-37	38.296471746988246	41.0	39.0	41.0	32.0	41.0
38-39	38.25097628097828	41.0	37.0	41.0	32.0	41.0
40-41	38.26296554618423	41.0	37.0	41.0	32.0	41.0
42-43	38.223114594694536	41.0	37.0	41.0	32.0	41.0
44-45	38.17798381218393	41.0	37.0	41.0	32.0	41.0
46-47	38.18392884478108	41.0	37.0	41.0	32.0	41.0
48-49	38.150269427014216	41.0	37.0	41.0	32.0	41.0
50-51	38.08883759489993	41.0	37.0	41.0	32.0	41.0
52-53	38.110782720865785	41.0	37.0	41.0	32.0	41.0
54-55	38.1109392764486	41.0	37.0	41.0	32.0	41.0
56-57	38.10230543004104	41.0	37.0	41.0	32.0	41.0
58-59	38.11567893597867	41.0	37.0	41.0	32.0	41.0
60-61	38.0796219831435	41.0	37.0	41.0	32.0	41.0
62-63	38.11162038377887	41.0	37.0	41.0	32.0	41.0
64-65	38.0984336590004	41.0	37.0	41.0	32.0	41.0
66-67	38.01452752552318	41.0	37.0	41.0	32.0	41.0
68-69	38.02719460324421	41.0	37.0	41.0	32.0	41.0
70-71	37.84730879616771	41.0	37.0	41.0	29.5	41.0
72-73	37.950417054221326	41.0	37.0	41.0	32.0	41.0
74-75	37.97523111620309	41.0	37.0	41.0	32.0	41.0
76	37.04372718261378	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24582.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	69.0
13	318.0
14	1425.0
15	5092.0
16	15115.0
17	32948.0
18	53169.0
19	71289.0
20	84574.0
21	96232.0
22	107038.0
23	119859.0
24	134511.0
25	150860.0
26	168690.0
27	191745.0
28	217538.0
29	247242.0
30	283841.0
31	327681.0
32	384382.0
33	455904.0
34	555389.0
35	703505.0
36	935666.0
37	1358768.0
38	2345185.0
39	5841140.0
40	9768988.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.69725993709846	19.087886641016894	14.228373831324785	30.986479590559863
2	22.26745213365122	27.233789001813406	28.645015845021305	21.85374301951407
3	21.61032346122769	28.184170565172657	27.519761811949987	22.68574416164967
4	25.3761305573099	32.552551902210816	19.224060026942322	22.847257513536963
5	24.683594510736352	34.599091827935766	19.99634025297515	20.720973408352737
6	20.136137611535343	36.75492865578546	20.81082761365271	22.298106119026485
7	20.250027475769617	21.511418767633664	33.460146345479316	24.778407411117406
8	21.194384268190262	23.634226590610243	26.02965269330718	29.141736447892313
9	22.089128561030723	23.478008155952736	27.233288531681527	27.199574751335014
10-11	23.828020610731276	30.758944642213805	21.24007847130243	24.17295627575249
12-13	23.54618299429429	25.067553800929872	25.455600353052	25.93066285172384
14-15	22.754142271468655	27.796789840745177	25.985489351156172	23.463578536629996
16-17	23.065755805343816	27.74123788651725	25.423285630513686	23.769720677625248
18-19	22.865900989764928	28.083608556430455	25.475148161384258	23.575342292420356
20-21	22.87198806110409	28.104854383027845	25.53159502709305	23.491562528775017
22-23	23.108745838105012	27.72703366686351	25.448882625269185	23.71533786976229
24-25	23.000957774010796	27.657830991365557	25.636315620553745	23.704895614069898
26-27	22.819513768680842	27.96278833337483	25.518511987558178	23.699185910386145
28-29	23.351859251554256	27.37593202875585	25.442120201862377	23.830088517827516
30-31	22.99844567073082	27.587559947210476	25.699293034282483	23.714701347776217
32-33	22.86174296701808	27.805358867833156	25.79265260852777	23.540245556620988
34-35	23.13065139040333	27.635955954237573	25.359005885459425	23.874386769899676
36-37	22.840340598447792	27.467874811278115	25.841362158506815	23.85042243176728
38-39	22.617289518208057	27.76613343163687	25.9794295667122	23.63714748344287
40-41	23.082718292062506	27.43522931460477	25.61032500884185	23.87172738449087
42-43	22.69536826498644	27.46964657601179	25.868211026733967	23.966774132267805
44-45	22.79953295817957	27.33292468493052	25.877809257381873	23.989733099508037
46-47	23.026350972744783	27.231664364179046	25.652599420876786	24.08938524219938
48-49	22.855514543136845	27.201673359734784	25.96271310568254	23.980098991445832
50-51	22.719510668282787	27.374980816823413	25.92597533483095	23.979533180062848
52-53	22.92413008311785	27.268236286552707	25.637925032776327	24.16970859755313
54-55	22.829765581152735	27.08322132612391	26.00530864391688	24.081704448806477
56-57	22.670435405330664	27.427461882231956	25.8084391990284	24.093663513408977
58-59	22.85746215217382	27.44768057388146	25.72023055088115	23.974626723063565
60-61	22.78662146599538	27.198668814083703	26.026100100305193	23.988609619615715
62-63	22.645686478529687	27.574168290923538	25.908045078623804	23.87210015192297
64-65	23.056399713788114	27.276301949507364	25.76989304897027	23.89740528773425
66-67	22.85477802460509	27.24533787868843	26.05934713382937	23.84053696287711
68-69	22.68601128680988	27.346853815466087	26.265699158204963	23.701435739519066
70-71	23.016970110736644	27.38895479431992	25.842038200535868	23.752036894407574
72-73	22.757191534849035	27.155108021203482	26.035712688907388	24.051987755040095
74-75	22.65993492314479	24.021840821015594	27.74602602002405	25.57219823581557
76	23.99612339209379	0.0	38.92091862551509	37.08295798239112
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	67075.0
1	39201.0
2	10548.0
3	9698.5
4	9628.0
5	9132.5
6	9923.0
7	12828.5
8	14455.5
9	15694.5
10	20267.5
11	48882.0
12	74165.0
13	96559.0
14	91133.5
15	52785.5
16	31559.5
17	21794.5
18	22032.0
19	23712.5
20	29654.0
21	35806.0
22	44178.0
23	59546.5
24	83125.0
25	105208.5
26	123656.5
27	153980.5
28	192295.0
29	217375.0
30	265243.5
31	344178.0
32	409232.0
33	447211.0
34	524056.5
35	628855.5
36	694280.0
37	728077.0
38	750729.5
39	807113.5
40	872007.0
41	913777.5
42	936624.5
43	993728.0
44	1092974.5
45	1165813.0
46	1203495.0
47	1256502.5
48	1296357.0
49	1290419.5
50	1277441.5
51	1266333.0
52	1260748.0
53	1238471.0
54	1202480.5
55	1125252.0
56	1009373.5
57	891486.0
58	811778.0
59	733831.5
60	627601.5
61	526931.5
62	452737.0
63	382417.0
64	301480.0
65	237974.0
66	180465.0
67	151067.0
68	130282.5
69	103386.5
70	80582.5
71	66938.0
72	60585.5
73	56190.0
74	46344.0
75	35642.0
76	29146.5
77	23050.5
78	19258.5
79	16864.5
80	15726.5
81	14063.0
82	12240.5
83	11093.5
84	9864.5
85	7689.5
86	5726.0
87	4922.5
88	4576.0
89	2975.5
90	1333.5
91	804.0
92	669.0
93	568.0
94	421.5
95	354.5
96	332.0
97	291.0
98	231.0
99	197.5
100	183.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.2509953309084816
2	0.23872141292545002
3	0.09959183633782076
4	0.10081941450112561
5	0.10251695658173526
6	0.10029273080069778
7	0.09989164090575658
8	0.10163779994332882
9	0.10198622146822725
10-11	0.10053378987897052
12-13	0.09975389286102931
14-15	0.10013877710364964
16-17	0.10063507520597587
18-19	0.10199027288130746
20-21	0.10069584640217907
22-23	0.09989771802537692
24-25	0.10571959862164444
26-27	0.10235490005852672
28-29	0.1047229510039118
30-31	0.10977303740839857
32-33	0.116180347194757
34-35	0.1227375592650834
36-37	0.003099166869711451
38-39	0.004052767355514736
40-41	0.0012516584220514705
42-43	0.0023190265558365396
44-45	0.0015908990509414603
46-47	1.928066243322853E-4
48-49	0.004169502496748438
50-51	0.004282147337908389
52-53	0.001699946832487239
54-55	0.010257893686897269
56-57	0.004324968191769415
58-59	0.004359325806678092
60-61	0.0011554452186741102
62-63	3.969032993960658E-4
64-65	7.474806947325911E-4
66-67	0.005460569257924537
68-69	0.0038638131923434565
70-71	0.004139168947582908
72-73	0.0015197268855949805
74-75	3.1060335774041396E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	30543.0
36	833.0
37	996.0
38	1101.0
39	1236.0
40	1475.0
41	1676.0
42	1850.0
43	1892.0
44	1977.0
45	1934.0
46	2300.0
47	2412.0
48	2570.0
49	2887.0
50	3137.0
51	3643.0
52	3734.0
53	4036.0
54	4397.0
55	4386.0
56	4788.0
57	5208.0
58	5553.0
59	5786.0
60	6252.0
61	7194.0
62	7545.0
63	7947.0
64	8658.0
65	9171.0
66	10439.0
67	10524.0
68	10399.0
69	11481.0
70	14389.0
71	37508.0
72	123017.0
73	563553.0
74	1791167.0
75	6123531.0
76	1.5839621E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.63570640925145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.68472419316572	24.37388732024075
2	11.576360836162642	7.556054270731212
3	4.404982710749309	4.312791674575291
4	2.257017062638045	2.9463738486770525
5	1.4061580559050102	2.294548073875985
6	0.942098664429819	1.8447633252527689
7	0.6727563523781696	1.5369115160821
8	0.5166237391136139	1.3488304539009293
9	0.39126726196945527	1.1492355140268136
>10	2.596017406412496	16.400500717563247
>50	0.28516387857817294	6.494747473836285
>100	0.2257308541668336	14.882088725130352
>500	0.02771707750594974	6.190041702335751
>1k	0.013106931270697335	7.053384448403207
>5k	1.7022296197759067E-4	0.3685246008137249
>10k+	1.0475259198619477E-4	1.2473163345543798
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106692	0.432253364354193	No Hit
CCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48669	0.1971782232009356	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33386	0.13526047709602487	No Hit
CCCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28310	0.11469550430085859	No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26601	0.10777163934677285	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.051413080214009E-6	4.051413080214009E-6
3	4.051413080214009E-6	0.0	0.0	4.051413080214009E-6	8.102826160428017E-6
4	8.102826160428017E-6	0.0	0.0	4.051413080214009E-6	8.102826160428017E-6
5	8.102826160428017E-6	0.0	0.0	4.051413080214009E-6	8.102826160428017E-6
6	8.102826160428017E-6	0.0	0.0	8.102826160428017E-6	8.102826160428017E-6
7	8.102826160428017E-6	0.0	0.0	3.241130464171207E-5	8.102826160428017E-6
8	8.102826160428017E-6	0.0	0.0	3.241130464171207E-5	8.102826160428017E-6
9	8.102826160428017E-6	0.0	0.0	3.646271772192608E-5	8.102826160428017E-6
10	1.6205652320856035E-5	0.0	0.0	3.646271772192608E-5	8.102826160428017E-6
11	1.6205652320856035E-5	0.0	0.0	3.646271772192608E-5	8.102826160428017E-6
12	2.0257065401070043E-5	0.0	0.0	3.646271772192608E-5	8.102826160428017E-6
13	2.835989156149806E-5	0.0	0.0	3.646271772192608E-5	8.102826160428017E-6
14	3.646271772192608E-5	0.0	0.0	3.646271772192608E-5	8.102826160428017E-6
15	4.4565543882354096E-5	0.0	0.0	3.646271772192608E-5	8.102826160428017E-6
16	4.4565543882354096E-5	0.0	0.0	3.646271772192608E-5	8.102826160428017E-6
17	4.8616956962568104E-5	0.0	0.0	3.646271772192608E-5	8.102826160428017E-6
18	4.8616956962568104E-5	0.0	0.0	3.646271772192608E-5	8.102826160428017E-6
19	4.8616956962568104E-5	0.0	0.0	3.646271772192608E-5	1.2154239240642026E-5
20	4.8616956962568104E-5	0.0	0.0	3.646271772192608E-5	1.2154239240642026E-5
21	4.8616956962568104E-5	0.0	0.0	3.646271772192608E-5	1.2154239240642026E-5
22	6.077119620321013E-5	0.0	0.0	3.646271772192608E-5	1.2154239240642026E-5
23	6.887402236363814E-5	0.0	0.0	4.4565543882354096E-5	1.2154239240642026E-5
24	7.697684852406616E-5	0.0	0.0	4.4565543882354096E-5	1.2154239240642026E-5
25	8.507967468449418E-5	0.0	0.0	4.4565543882354096E-5	1.6205652320856035E-5
26	9.723391392513621E-5	0.0	0.0	4.4565543882354096E-5	1.6205652320856035E-5
27	1.0938815316577823E-4	0.0	0.0	4.4565543882354096E-5	1.6205652320856035E-5
28	1.2154239240642026E-4	0.0	0.0	4.4565543882354096E-5	1.6205652320856035E-5
29	1.2964521856684828E-4	0.0	0.0	5.671978312299612E-5	1.6205652320856035E-5
30	1.3774804472727628E-4	0.0	0.0	6.482260928342414E-5	1.6205652320856035E-5
31	1.458508708877043E-4	0.0	0.0	6.482260928342414E-5	2.0257065401070043E-5
32	1.7015934936898835E-4	0.0	0.0	6.482260928342414E-5	2.0257065401070043E-5
33	1.8231358860963038E-4	0.0	0.0	6.482260928342414E-5	2.0257065401070043E-5
34	1.9446782785027242E-4	0.0	0.0	6.887402236363814E-5	2.0257065401070043E-5
35	2.1877630633155646E-4	0.0	0.0	7.697684852406616E-5	2.0257065401070043E-5
36	2.2687913249198449E-4	0.0	0.0	8.913108776470819E-5	2.0257065401070043E-5
37	2.4308478481284052E-4	0.0	0.0	9.318250084492219E-5	2.0257065401070043E-5
38	2.5118761097326855E-4	8.102826160428017E-6	0.0	1.0128532700535021E-4	2.0257065401070043E-5
39	2.6334185021391056E-4	8.102826160428017E-6	0.0	1.0533674008556423E-4	2.0257065401070043E-5
40	3.0385598101605063E-4	8.102826160428017E-6	0.0	1.0533674008556423E-4	2.0257065401070043E-5
41	3.281644594973347E-4	8.102826160428017E-6	0.0	1.0533674008556423E-4	2.0257065401070043E-5
42	3.403186987379767E-4	8.102826160428017E-6	0.0	1.1749097932620625E-4	2.0257065401070043E-5
43	3.5652435105883276E-4	8.102826160428017E-6	0.0	1.1749097932620625E-4	2.0257065401070043E-5
44	3.7273000337968877E-4	8.102826160428017E-6	0.0	1.2154239240642026E-4	2.0257065401070043E-5
45	3.8488424262033083E-4	8.102826160428017E-6	0.0	1.2559380548663428E-4	2.0257065401070043E-5
46	4.0919272110161484E-4	8.102826160428017E-6	0.0	1.2964521856684828E-4	2.0257065401070043E-5
47	4.172955472620429E-4	8.102826160428017E-6	0.0	1.3774804472727628E-4	2.0257065401070043E-5
48	4.4565543882354097E-4	8.102826160428017E-6	0.0	1.458508708877043E-4	2.0257065401070043E-5
49	4.4970685190375497E-4	8.102826160428017E-6	0.0	1.4990228396791831E-4	2.0257065401070043E-5
50	4.8616956962568104E-4	8.102826160428017E-6	0.0	1.5800511012834635E-4	2.0257065401070043E-5
51	4.942723957861091E-4	8.102826160428017E-6	0.0	1.6205652320856035E-4	2.0257065401070043E-5
52	5.145294611871791E-4	8.102826160428017E-6	0.0	1.6610793628877435E-4	2.0257065401070043E-5
53	5.428893527486771E-4	8.102826160428017E-6	0.0	1.7421076244920238E-4	2.0257065401070043E-5
54	5.590950050695332E-4	8.102826160428017E-6	0.0	1.7826217552941638E-4	2.0257065401070043E-5
55	5.915063097112453E-4	8.102826160428017E-6	0.0	1.8231358860963038E-4	2.4308478481284052E-5
56	6.158147881925293E-4	8.102826160428017E-6	0.0	1.8231358860963038E-4	2.4308478481284052E-5
57	6.320204405133854E-4	1.2154239240642026E-5	0.0	2.1472489325134245E-4	2.4308478481284052E-5
58	6.765859843957395E-4	1.2154239240642026E-5	0.0	2.1877630633155646E-4	2.4308478481284052E-5
59	7.373571805989495E-4	1.2154239240642026E-5	0.0	2.349819586524125E-4	2.4308478481284052E-5
60	7.819227244813036E-4	1.2154239240642026E-5	0.0	2.349819586524125E-4	2.4308478481284052E-5
61	8.102826160428017E-4	1.2154239240642026E-5	0.0	2.3903337173262652E-4	2.4308478481284052E-5
62	8.264882683636578E-4	1.2154239240642026E-5	0.0	2.4308478481284052E-4	2.4308478481284052E-5
63	8.264882683636578E-4	1.2154239240642026E-5	0.0	2.5118761097326855E-4	2.4308478481284052E-5
64	8.264882683636578E-4	1.2154239240642026E-5	0.0	2.5118761097326855E-4	2.4308478481284052E-5
65	8.305396814438717E-4	1.6205652320856035E-5	0.0	2.5523902405348255E-4	2.835989156149806E-5
>>END_MODULE
