##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543826_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24682746
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.512130660016513	32.0	32.0	32.0	32.0	32.0
2	31.74435603720915	32.0	32.0	32.0	32.0	32.0
3	35.90134570116307	37.0	37.0	37.0	32.0	37.0
4	36.441138558894544	37.0	37.0	37.0	37.0	37.0
5	36.570939513780196	37.0	37.0	37.0	37.0	37.0
6	40.099364633092286	41.0	41.0	41.0	37.0	41.0
7	40.241279475144296	41.0	41.0	41.0	37.0	41.0
8	40.23522524600788	41.0	41.0	41.0	37.0	41.0
9	40.18173383139785	41.0	41.0	41.0	37.0	41.0
10-11	39.895670096836064	41.0	41.0	41.0	37.0	41.0
12-13	39.756763084626	41.0	41.0	41.0	37.0	41.0
14-15	39.865033108552836	41.0	41.0	41.0	37.0	41.0
16-17	39.82080845056705	41.0	41.0	41.0	37.0	41.0
18-19	39.75335576519727	41.0	41.0	41.0	37.0	41.0
20-21	39.71164958712454	41.0	41.0	41.0	37.0	41.0
22-23	39.687406660506895	41.0	41.0	41.0	37.0	41.0
24-25	39.655414292234745	41.0	41.0	41.0	37.0	41.0
26-27	39.532565217824626	41.0	41.0	41.0	37.0	41.0
28-29	39.53655976932227	41.0	41.0	41.0	37.0	41.0
30-31	39.56928690187064	41.0	41.0	41.0	37.0	41.0
32-33	39.629963740663214	41.0	41.0	41.0	37.0	41.0
34-35	39.626452239147135	41.0	41.0	41.0	37.0	41.0
36-37	39.7795612149583	41.0	41.0	41.0	37.0	41.0
38-39	39.85241819950863	41.0	41.0	41.0	37.0	41.0
40-41	39.92066625785388	41.0	41.0	41.0	37.0	41.0
42-43	39.93310164646624	41.0	41.0	41.0	37.0	41.0
44-45	39.94363839785444	41.0	41.0	41.0	37.0	41.0
46-47	39.86444746713713	41.0	41.0	41.0	37.0	41.0
48-49	39.93123738922702	41.0	41.0	41.0	37.0	41.0
50-51	39.91285412732682	41.0	41.0	41.0	37.0	41.0
52-53	39.86038782208504	41.0	41.0	41.0	37.0	41.0
54-55	39.89730926436588	41.0	41.0	41.0	37.0	41.0
56-57	39.89909687002539	41.0	41.0	41.0	37.0	41.0
58-59	39.82788320510595	41.0	41.0	41.0	37.0	41.0
60-61	39.86323332326593	41.0	41.0	41.0	37.0	41.0
62-63	39.84234230804826	41.0	41.0	41.0	37.0	41.0
64-65	39.78418118535967	41.0	41.0	41.0	37.0	41.0
66-67	39.78403489289717	41.0	41.0	41.0	37.0	41.0
68-69	39.772370590293576	41.0	41.0	41.0	37.0	41.0
70-71	39.75403029938868	41.0	41.0	41.0	37.0	41.0
72-73	39.70128356043088	41.0	41.0	41.0	37.0	41.0
74-75	39.67960951516225	41.0	41.0	41.0	37.0	41.0
76	39.199800916084556	41.0	41.0	41.0	37.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25088.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	2.0
14	4.0
15	3.0
16	9.0
17	24.0
18	69.0
19	167.0
20	378.0
21	880.0
22	2131.0
23	3855.0
24	6759.0
25	11802.0
26	19921.0
27	32443.0
28	49731.0
29	73354.0
30	104058.0
31	144086.0
32	193643.0
33	258438.0
34	340923.0
35	452092.0
36	613914.0
37	877842.0
38	1472272.0
39	4178799.0
40	1.5820059E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.966798067302584	10.025550956556014	11.916841020222831	36.090809955918566
2	23.048647140416474	14.50289561384522	33.12652116406947	29.32193608166883
3	22.57570041729024	19.181691951441618	27.018441897442163	31.224165733825977
4	27.37630593848952	26.135086416876945	20.53523077217593	25.9533768724576
5	26.65097390838984	29.297336348813012	24.096911393612483	19.95477834918466
6	21.996601130993454	31.891743459038658	26.576752316748898	19.534903093218993
7	18.681275407421094	22.262016936077224	41.59261597350405	17.464091682997633
8	21.338298456856737	22.030969444950713	33.27675035934163	23.35398173885092
9	21.4018500864924	21.66509487640716	34.83959019952341	22.09346483757703
10-11	24.2515570619075	29.498955253576796	26.09029008351077	20.15919760100493
12-13	23.891548125747832	23.6847048234244	30.160624809731235	22.263122241096536
14-15	23.109552259543715	25.453621601865645	30.211657568934804	21.225168569655835
16-17	23.420978185650227	25.7469690023962	29.472706781534026	21.359346030419545
18-19	23.435174118642383	26.025923267522334	29.24322225201013	21.295680361825152
20-21	23.09946873894296	25.908469020255232	29.61768074303427	21.37438149776754
22-23	23.520264236820015	25.669812339232994	29.162870335440083	21.647053088506908
24-25	23.438281558811894	25.795963041869204	29.07407550480024	21.691679894518664
26-27	23.214338115740453	26.090845827271796	29.064962058087524	21.629853998900227
28-29	23.56389051439875	25.859947557576092	29.003818041276787	21.572343886748378
30-31	23.258325978978576	26.010814039817287	28.889082404675566	21.84177757652857
32-33	23.187115909833096	25.76523962010197	29.109334072743685	21.93831039732125
34-35	23.321020909581193	25.89080407142548	28.93606984845884	21.852105170534482
36-37	23.712250365072055	25.711747511226385	28.68114287531634	21.894859248385217
38-39	23.30739224470879	26.078037715163312	28.974869918614747	21.63970012151315
40-41	23.61241896825067	25.532969056040344	29.048733160966	21.805878814742986
42-43	23.243396738542714	25.33904614996188	29.395465036212602	22.022092075282803
44-45	23.411198360130502	25.41521544462067	29.403324702451922	21.77026149279691
46-47	23.416620076637056	25.617633402931368	29.15066932453932	21.815077195892265
48-49	23.40619107979407	25.56822369762829	28.993110297498877	22.032474925078766
50-51	23.201212131475	25.731597295254087	29.103277039586168	21.96391353368475
52-53	23.403206848721013	25.56136181843558	28.923200076560505	22.112231256282893
54-55	23.244689797943643	25.818538038807315	28.746588356099164	22.19018380714988
56-57	23.533167936787162	25.489112712865676	29.056305987892074	21.92141336245509
58-59	23.46729501930012	25.412194768902108	28.990125739048665	22.130384472749103
60-61	23.134868670377973	25.465401253866588	29.10290771301226	22.29682236274318
62-63	23.017398430856844	25.69452789738008	29.26858916176778	22.0194845099953
64-65	23.45345558373745	25.63896292823034	28.86875849777236	22.038822990259842
66-67	23.55271337890986	25.51374487765449	28.765867013697964	22.167674729737683
68-69	23.274536132264675	25.889022198791444	28.862047668933922	21.974394000009955
70-71	23.56877324179099	25.711858042667807	28.702486086828156	22.01688262871305
72-73	23.461344666690252	25.347374230226006	28.873296713806678	22.317984389277065
74-75	23.601441495069995	22.250481022092572	30.49584229751164	23.6522351853258
76	24.57317202613431	0.0	42.1255496265693	33.30127834729639
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	339021.0
1	239192.5
2	88352.0
3	34465.0
4	31590.0
5	36128.5
6	50426.5
7	70767.5
8	81356.5
9	80957.5
10	90310.5
11	85677.0
12	71282.5
13	59318.5
14	30582.0
15	11714.5
16	9369.5
17	9576.0
18	11212.0
19	15597.5
20	21409.0
21	26022.0
22	33041.5
23	44932.0
24	59415.0
25	72891.5
26	100462.5
27	143822.5
28	184360.5
29	219362.0
30	275742.0
31	341459.5
32	400127.0
33	450033.0
34	520678.0
35	629970.5
36	721681.5
37	762600.0
38	773251.5
39	815233.5
40	880740.5
41	923570.5
42	942955.5
43	983356.5
44	1056819.5
45	1141778.0
46	1190176.5
47	1225084.0
48	1253740.5
49	1251546.0
50	1243751.5
51	1235507.0
52	1239388.0
53	1203997.0
54	1161975.0
55	1105038.5
56	1001130.0
57	888735.5
58	810192.5
59	731514.5
60	625485.0
61	514576.5
62	431857.0
63	368243.0
64	290130.5
65	229026.0
66	170246.5
67	135278.0
68	109294.0
69	79721.5
70	61637.0
71	51904.0
72	45197.5
73	37550.0
74	29571.5
75	23117.5
76	19940.5
77	15869.0
78	13485.0
79	13323.0
80	11170.0
81	9413.5
82	8495.0
83	7052.0
84	6220.0
85	5301.0
86	5165.5
87	5200.0
88	3498.0
89	1481.5
90	623.0
91	213.0
92	143.0
93	116.5
94	74.5
95	51.0
96	44.0
97	31.5
98	11.5
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9145497830751895
2	0.16048457493343732
3	0.10164185135640905
4	0.10903973164087984
5	0.10164185135640905
6	0.10182011353193847
7	0.10164185135640905
8	0.10190114179354273
9	0.10164185135640905
10-11	0.10164185135640905
12-13	0.1020611726102112
14-15	0.10183226777117911
16-17	0.10187885902160157
18-19	0.10165197988910958
20-21	0.10166818554143045
22-23	0.10196596440282617
24-25	0.10328064794735561
26-27	0.11191420922129167
28-29	0.11379811630359117
30-31	0.11284198281666068
32-33	0.1169521413865378
34-35	0.12156062376528123
36-37	5.07070119238147E-4
38-39	0.0014097631023940555
40-41	3.5501188021899163E-4
42-43	6.086730924555558E-6
44-45	0.0
46-47	2.8413702946628857E-5
48-49	7.023610190446408E-4
50-51	5.218166040012978E-4
52-53	2.355952399037894E-4
54-55	1.8488094804674701E-4
56-57	0.001758022134453899
58-59	0.002080015576327788
60-61	1.0171081041281917E-5
62-63	0.002784400906835411
64-65	0.0
66-67	0.0012290359686446223
68-69	0.0012626837758210419
70-71	0.0
72-73	3.484553456216863E-5
74-75	5.563304823859033E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	30917.0
36	812.0
37	954.0
38	1063.0
39	1207.0
40	1461.0
41	1650.0
42	1823.0
43	1875.0
44	1949.0
45	1903.0
46	2263.0
47	2397.0
48	2529.0
49	2877.0
50	3112.0
51	3600.0
52	3720.0
53	4016.0
54	4372.0
55	4360.0
56	4751.0
57	5187.0
58	5572.0
59	5765.0
60	6239.0
61	7188.0
62	7512.0
63	7909.0
64	8629.0
65	8815.0
66	9794.0
67	10101.0
68	10281.0
69	11192.0
70	12932.0
71	38473.0
72	100353.0
73	449669.0
74	1771234.0
75	5776419.0
76	1.6345871E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.68889239646383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.83539853917769	18.371405446798168
2	14.088030100249881	7.519878388474253
3	5.439875342522509	4.355527430002802
4	2.832344303913104	3.023685294274964
5	1.7242182082855213	2.3008737114477973
6	1.1822441232638563	1.893167171528447
7	0.8321073190014338	1.5545615889397488
8	0.627000747884795	1.3387164394239712
9	0.4869050683388261	1.1695461278569017
>10	3.2616083486450855	16.864545516119225
>50	0.3577067734677091	6.6630673126247455
>100	0.2807111176786006	15.056947517332587
>500	0.03192235061423049	5.912886563281347
>1k	0.01920546833335269	9.122754855943219
>5k	4.0121590178979585E-4	0.6349034953546152
>10k+	3.20972721431825E-4	4.217533140596897
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	286277	1.1598263823644257	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	113080	0.4581337911106001	No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	91538	0.3708582505366299	No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74837	0.3031956006839758	No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71135	0.28819726946102353	No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59299	0.2402447442436105	No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53954	0.21858994132986664	No Hit
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34016	0.13781286733655973	No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33009	0.1337330943647842	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	25088	0.10164185135640905	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.2154239240642026E-5	1.2154239240642026E-5
3	8.102826160428017E-6	0.0	0.0	1.6205652320856035E-5	1.6205652320856035E-5
4	2.4308478481284052E-5	0.0	0.0	1.6205652320856035E-5	1.6205652320856035E-5
5	3.241130464171207E-5	0.0	0.0	2.0257065401070043E-5	2.0257065401070043E-5
6	3.241130464171207E-5	0.0	0.0	2.4308478481284052E-5	2.0257065401070043E-5
7	4.051413080214009E-5	0.0	0.0	2.4308478481284052E-5	2.4308478481284052E-5
8	4.051413080214009E-5	0.0	0.0	2.835989156149806E-5	3.646271772192608E-5
9	4.8616956962568104E-5	0.0	0.0	3.241130464171207E-5	4.051413080214009E-5
10	5.671978312299612E-5	0.0	0.0	3.646271772192608E-5	4.051413080214009E-5
11	6.887402236363814E-5	0.0	0.0	4.051413080214009E-5	4.051413080214009E-5
12	6.887402236363814E-5	0.0	0.0	4.051413080214009E-5	4.051413080214009E-5
13	6.887402236363814E-5	8.102826160428017E-6	0.0	4.4565543882354096E-5	4.051413080214009E-5
14	6.887402236363814E-5	8.102826160428017E-6	0.0	4.8616956962568104E-5	4.051413080214009E-5
15	8.102826160428017E-5	8.102826160428017E-6	0.0	5.671978312299612E-5	4.051413080214009E-5
16	8.102826160428017E-5	8.102826160428017E-6	0.0	5.671978312299612E-5	4.051413080214009E-5
17	8.913108776470819E-5	8.102826160428017E-6	0.0	5.671978312299612E-5	4.4565543882354096E-5
18	8.913108776470819E-5	8.102826160428017E-6	0.0	6.482260928342414E-5	4.8616956962568104E-5
19	9.318250084492219E-5	8.102826160428017E-6	0.0	6.482260928342414E-5	4.8616956962568104E-5
20	1.0128532700535021E-4	8.102826160428017E-6	0.0	6.482260928342414E-5	4.8616956962568104E-5
21	1.0128532700535021E-4	8.102826160428017E-6	0.0	6.887402236363814E-5	4.8616956962568104E-5
22	1.0533674008556423E-4	8.102826160428017E-6	0.0	7.292543544385216E-5	5.266837004278211E-5
23	1.1343956624599224E-4	1.6205652320856035E-5	0.0	7.697684852406616E-5	5.266837004278211E-5
24	1.1749097932620625E-4	1.6205652320856035E-5	4.051413080214009E-6	7.697684852406616E-5	5.266837004278211E-5
25	1.3774804472727628E-4	2.0257065401070043E-5	4.051413080214009E-6	7.697684852406616E-5	6.482260928342414E-5
26	1.6610793628877435E-4	2.0257065401070043E-5	4.051413080214009E-6	7.697684852406616E-5	6.887402236363814E-5
27	1.7421076244920238E-4	2.0257065401070043E-5	4.051413080214009E-6	7.697684852406616E-5	7.697684852406616E-5
28	1.8231358860963038E-4	2.0257065401070043E-5	4.051413080214009E-6	7.697684852406616E-5	7.697684852406616E-5
29	1.8636500168984439E-4	2.0257065401070043E-5	4.051413080214009E-6	8.102826160428017E-5	7.697684852406616E-5
30	1.8636500168984439E-4	2.4308478481284052E-5	4.051413080214009E-6	8.102826160428017E-5	7.697684852406616E-5
31	1.9041641477005842E-4	2.4308478481284052E-5	4.051413080214009E-6	8.102826160428017E-5	7.697684852406616E-5
32	1.9446782785027242E-4	2.4308478481284052E-5	4.051413080214009E-6	8.507967468449418E-5	8.102826160428017E-5
33	2.349819586524125E-4	2.4308478481284052E-5	4.051413080214009E-6	8.507967468449418E-5	8.102826160428017E-5
34	2.3903337173262652E-4	2.4308478481284052E-5	4.051413080214009E-6	8.507967468449418E-5	8.102826160428017E-5
35	2.4308478481284052E-4	2.4308478481284052E-5	4.051413080214009E-6	8.913108776470819E-5	8.507967468449418E-5
36	2.4713619789305455E-4	2.4308478481284052E-5	4.051413080214009E-6	8.913108776470819E-5	9.723391392513621E-5
37	2.5929043713369656E-4	2.4308478481284052E-5	4.051413080214009E-6	8.913108776470819E-5	9.723391392513621E-5
38	2.5929043713369656E-4	2.4308478481284052E-5	4.051413080214009E-6	9.318250084492219E-5	1.0533674008556423E-4
39	2.6334185021391056E-4	2.4308478481284052E-5	4.051413080214009E-6	9.318250084492219E-5	1.2964521856684828E-4
40	2.6739326329412456E-4	2.4308478481284052E-5	8.102826160428017E-6	9.318250084492219E-5	1.458508708877043E-4
41	2.6739326329412456E-4	2.4308478481284052E-5	8.102826160428017E-6	9.318250084492219E-5	1.5800511012834635E-4
42	3.0385598101605063E-4	2.4308478481284052E-5	8.102826160428017E-6	9.318250084492219E-5	1.5800511012834635E-4
43	3.119588071764787E-4	2.835989156149806E-5	8.102826160428017E-6	9.723391392513621E-5	1.7015934936898835E-4
44	3.200616333369067E-4	2.835989156149806E-5	8.102826160428017E-6	9.723391392513621E-5	1.7015934936898835E-4
45	3.241130464171207E-4	2.835989156149806E-5	8.102826160428017E-6	9.723391392513621E-5	1.7015934936898835E-4
46	3.4842152489840476E-4	2.835989156149806E-5	8.102826160428017E-6	9.723391392513621E-5	1.7015934936898835E-4
47	3.5247293797861876E-4	2.835989156149806E-5	8.102826160428017E-6	1.0128532700535021E-4	1.7421076244920238E-4
48	3.5652435105883276E-4	2.835989156149806E-5	8.102826160428017E-6	1.0128532700535021E-4	1.7826217552941638E-4
49	3.6462717721926077E-4	2.835989156149806E-5	1.2154239240642026E-5	1.0128532700535021E-4	1.8636500168984439E-4
50	3.6462717721926077E-4	2.835989156149806E-5	1.2154239240642026E-5	1.0128532700535021E-4	1.9041641477005842E-4
51	3.6867859029947477E-4	2.835989156149806E-5	1.2154239240642026E-5	1.0128532700535021E-4	1.9851924093048642E-4
52	3.767814164599028E-4	2.835989156149806E-5	1.2154239240642026E-5	1.0128532700535021E-4	2.0662206709091445E-4
53	3.9298706878075884E-4	2.835989156149806E-5	1.2154239240642026E-5	1.0533674008556423E-4	2.0662206709091445E-4
54	3.9703848186097284E-4	2.835989156149806E-5	1.2154239240642026E-5	1.0533674008556423E-4	2.0662206709091445E-4
55	4.0514130802140084E-4	2.835989156149806E-5	1.2154239240642026E-5	1.0533674008556423E-4	2.0662206709091445E-4
56	4.0919272110161484E-4	2.835989156149806E-5	1.2154239240642026E-5	1.0533674008556423E-4	2.1067348017112845E-4
57	4.172955472620429E-4	2.835989156149806E-5	1.2154239240642026E-5	1.0533674008556423E-4	2.1067348017112845E-4
58	4.416040257433269E-4	2.835989156149806E-5	1.2154239240642026E-5	1.1749097932620625E-4	2.1067348017112845E-4
59	4.5375826498396897E-4	2.835989156149806E-5	1.2154239240642026E-5	1.2964521856684828E-4	2.1067348017112845E-4
60	4.69963917304825E-4	2.835989156149806E-5	1.2154239240642026E-5	1.3774804472727628E-4	2.1067348017112845E-4
61	4.74015330385039E-4	2.835989156149806E-5	1.2154239240642026E-5	1.417994578074903E-4	2.1472489325134245E-4
62	4.74015330385039E-4	2.835989156149806E-5	1.2154239240642026E-5	1.417994578074903E-4	2.1472489325134245E-4
63	4.74015330385039E-4	2.835989156149806E-5	1.2154239240642026E-5	1.458508708877043E-4	2.1472489325134245E-4
64	4.74015330385039E-4	2.835989156149806E-5	1.2154239240642026E-5	1.458508708877043E-4	2.2282771941177048E-4
65	4.7806674346525304E-4	3.241130464171207E-5	1.6205652320856035E-5	1.4990228396791831E-4	2.2687913249198449E-4
>>END_MODULE
