##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543825_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23851221
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.50397323474551	32.0	32.0	32.0	32.0	32.0
2	30.771441093099593	32.0	32.0	32.0	32.0	32.0
3	33.65758306461544	37.0	32.0	37.0	27.0	37.0
4	34.747115545992386	37.0	37.0	37.0	27.0	37.0
5	35.29064432382728	37.0	37.0	37.0	32.0	37.0
6	38.46939936534067	41.0	41.0	41.0	32.0	41.0
7	38.44692512806787	41.0	41.0	41.0	32.0	41.0
8	38.50002995653765	41.0	41.0	41.0	32.0	41.0
9	38.618338239371475	41.0	41.0	41.0	32.0	41.0
10-11	38.578735025766605	41.0	41.0	41.0	32.0	41.0
12-13	38.43193692264224	41.0	41.0	41.0	32.0	41.0
14-15	38.42196296784974	41.0	41.0	41.0	32.0	41.0
16-17	38.29016403395029	41.0	41.0	41.0	32.0	41.0
18-19	38.24577307803236	41.0	41.0	41.0	32.0	41.0
20-21	38.069066443181256	41.0	39.0	41.0	32.0	41.0
22-23	37.94880218081917	41.0	37.0	41.0	32.0	41.0
24-25	38.05904538388202	41.0	37.0	41.0	32.0	41.0
26-27	38.21953242980726	41.0	41.0	41.0	32.0	41.0
28-29	38.17398463583898	41.0	41.0	41.0	32.0	41.0
30-31	38.30533334121553	41.0	41.0	41.0	32.0	41.0
32-33	38.47010427684184	41.0	41.0	41.0	32.0	41.0
34-35	38.444005361402674	41.0	41.0	41.0	32.0	41.0
36-37	38.4920101818696	41.0	41.0	41.0	32.0	41.0
38-39	38.50219076822029	41.0	41.0	41.0	32.0	41.0
40-41	38.53744068884009	41.0	41.0	41.0	32.0	41.0
42-43	38.48908861061568	41.0	41.0	41.0	32.0	41.0
44-45	38.44717576398834	41.0	41.0	41.0	32.0	41.0
46-47	38.46124219139294	41.0	41.0	41.0	32.0	41.0
48-49	38.43211692635553	41.0	41.0	41.0	32.0	41.0
50-51	38.37821436250561	41.0	41.0	41.0	32.0	41.0
52-53	38.39114768586211	41.0	41.0	41.0	32.0	41.0
54-55	38.37762873171363	41.0	41.0	41.0	32.0	41.0
56-57	38.365470285469364	41.0	41.0	41.0	32.0	41.0
58-59	38.37597683032859	41.0	41.0	41.0	32.0	41.0
60-61	38.3382557535304	41.0	41.0	41.0	32.0	41.0
62-63	38.363493287914174	41.0	41.0	41.0	32.0	41.0
64-65	38.34462178420132	41.0	41.0	41.0	32.0	41.0
66-67	38.26073142550693	41.0	37.0	41.0	32.0	41.0
68-69	38.26985961885547	41.0	37.0	41.0	32.0	41.0
70-71	38.093908988823834	41.0	37.0	41.0	32.0	41.0
72-73	38.195379757739374	41.0	37.0	41.0	32.0	41.0
74-75	38.19912103846133	41.0	37.0	41.0	32.0	41.0
76	37.213502896729665	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62871.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	46.0
13	296.0
14	1458.0
15	5404.0
16	14819.0
17	31010.0
18	49245.0
19	66108.0
20	78290.0
21	87905.0
22	97499.0
23	109337.0
24	122222.0
25	137362.0
26	155428.0
27	175498.0
28	199922.0
29	228281.0
30	261247.0
31	302002.0
32	353947.0
33	422650.0
34	519517.0
35	659858.0
36	879054.0
37	1258835.0
38	2107975.0
39	5103570.0
40	1.0359565E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.67948495865938	19.360071953904658	14.160387577318954	30.80005551011701
2	22.324401745695486	27.496535023883865	28.535895258270955	21.643167972149698
3	21.547164893740003	28.757198376516236	27.173473570045843	22.522163159697918
4	25.503078621600455	32.75361724218107	19.18309005253513	22.560214083683334
5	24.75163085491702	34.87151771902738	19.904595980515456	20.472255445540142
6	20.133344296754938	36.76020129985488	20.880164336981615	22.226290066408573
7	20.430682581783426	21.690630493118775	33.2774094475495	24.601277477548297
8	21.180778810464634	23.74259460474284	26.065335000455903	29.011291584336618
9	22.214285371973013	23.624795661838725	27.220104747926392	26.940814218261867
10-11	24.055574344100535	30.690598485337333	21.09464655383871	24.15918061672342
12-13	23.813119510805425	25.1163616977504	25.069090611924523	26.001428179519653
14-15	22.935760456072767	27.925868690940174	25.648558888692037	23.48981196429502
16-17	23.271285911698158	27.858207389002644	25.089018689894505	23.781488009404693
18-19	23.01667344746429	28.256460191589273	25.155746051267876	23.571120309678566
20-21	23.02061493992565	28.354609445195067	25.141363440533947	23.48341217434534
22-23	23.24208248411343	27.990470170498227	25.092840464307308	23.674606881081033
24-25	23.154307742817252	27.906192647451494	25.27062315429513	23.66887645543612
26-27	22.988432541501723	28.190558178290814	25.15475875635055	23.666250523856913
28-29	23.561864196168187	27.592796141856002	25.089463445504066	23.75587621647174
30-31	23.15270455465439	27.759463237904512	25.410241955976602	23.677590251464494
32-33	23.047984749991233	27.97018148321102	25.47451087142535	23.507322895372397
34-35	23.321770783128397	27.795949762272095	25.05584423909965	23.82643521549986
36-37	23.02014582847953	27.575071639985836	25.540847198029155	23.863935333505477
38-39	22.791457091508537	27.852566117263304	25.716466763722835	23.639510027505324
40-41	23.27493583351814	27.53919060213012	25.313616098846165	23.872257465505573
42-43	22.907754484177033	27.523931658868072	25.6022104740567	23.96610338289819
44-45	23.000655888657587	27.4164362709584	25.615665238805967	23.967242601578047
46-47	23.256796190253127	27.31143587902844	25.385148633176403	24.046619297542033
48-49	23.059150280249035	27.275608380067823	25.675561721901115	23.989679617782027
50-51	22.912371052590004	27.45144837101046	25.66006224230265	23.97611833409688
52-53	23.123867776671727	27.330869652459413	25.37396164613297	24.17130092473589
54-55	23.007955032571665	27.152445166541895	25.753117190628842	24.086482610257594
56-57	22.884241688442728	27.50166379676142	25.561496595103012	24.05259791969284
58-59	23.059000583832244	27.51521372497492	25.4941234317175	23.931662259475335
60-61	22.996615782294946	27.260619795680025	25.755987057792396	23.986777364232633
62-63	22.855747415916948	27.645147882930814	25.66136046509857	23.837744236053666
64-65	23.23596139761621	27.36037122898311	25.5152849782785	23.88838239512218
66-67	23.078422991775145	27.308684205281768	25.810207947203516	23.80268485573957
68-69	22.907195991760133	27.386841500052995	25.986000442974504	23.719962065212368
70-71	23.216080057909448	27.432165623672677	25.57825181188719	23.77350250653069
72-73	22.930637986920967	27.21409419676823	25.78251647874467	24.072751337566135
74-75	22.87674348397736	24.068970551439286	27.47512947636671	25.579156488216647
76	24.305771456178196	0.0	38.55395566774023	37.14027287608157
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	105667.0
1	59095.0
2	11597.5
3	10379.0
4	10086.0
5	9706.0
6	10343.0
7	12905.0
8	14452.0
9	15658.0
10	19367.5
11	41771.0
12	61696.0
13	78151.0
14	72738.0
15	43191.5
16	28016.0
17	21185.5
18	21607.0
19	23459.0
20	28809.0
21	34680.0
22	42331.5
23	55857.5
24	76335.5
25	96085.5
26	115922.0
27	146248.5
28	181908.0
29	206774.5
30	253242.5
31	327477.0
32	388700.5
33	426049.0
34	496581.5
35	593128.0
36	655337.5
37	687913.0
38	712827.5
39	772703.0
40	838937.0
41	879856.0
42	903212.5
43	958745.5
44	1057405.5
45	1134463.5
46	1173587.0
47	1219051.5
48	1255257.5
49	1252040.5
50	1238703.5
51	1229724.0
52	1227085.5
53	1212207.5
54	1181431.5
55	1105394.5
56	993751.5
57	878441.5
58	800379.0
59	721062.5
60	612994.5
61	512970.5
62	439571.5
63	371043.5
64	290842.0
65	228938.5
66	174343.0
67	146183.0
68	127140.5
69	100417.0
70	77213.0
71	64090.0
72	57647.5
73	53089.5
74	43541.5
75	33598.0
76	27789.0
77	22073.0
78	18810.0
79	16820.5
80	15808.5
81	14235.5
82	12469.5
83	11424.0
84	10124.5
85	7891.5
86	5977.0
87	5165.0
88	4699.0
89	2932.5
90	1239.0
91	739.5
92	644.5
93	541.0
94	418.5
95	364.5
96	330.5
97	321.0
98	268.0
99	210.0
100	196.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.4187533627733355
2	0.4010109167996053
3	0.26359656807506837
4	0.2647243929356908
5	0.2664182265553617
6	0.26425062264107985
7	0.2638690908109065
8	0.26551261254088415
9	0.26601153878034167
10-11	0.2644979894320714
12-13	0.2636971918544547
14-15	0.26413532456053296
16-17	0.2645503976505018
18-19	0.2660618506700349
20-21	0.2647977644414934
22-23	0.2640011595213511
24-25	0.2700385862845345
26-27	0.2671309783260153
28-29	0.27063813630337835
30-31	0.27769228250411165
32-33	0.2868406611133241
34-35	0.29586535632704086
36-37	0.0030089150304473396
38-39	0.004037593443738943
40-41	0.0010852742004158198
42-43	0.002267778961057249
44-45	0.001607606283922544
46-47	1.9363737066549589E-4
48-49	0.00409294962267489
50-51	0.004016626178499078
52-53	0.0016225909160870156
54-55	0.009991791508187449
56-57	0.004213114566990241
58-59	0.004222381607744844
60-61	0.0011874529495538926
62-63	3.3204538497838944E-4
64-65	7.050563721152873E-4
66-67	0.0054314940411931125
68-69	0.003908570697265022
70-71	0.003944658587779052
72-73	0.0014534136883014132
74-75	3.035778983512091E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	71331.0
36	1109.0
37	1409.0
38	1665.0
39	1834.0
40	2158.0
41	2574.0
42	2785.0
43	2814.0
44	3010.0
45	3030.0
46	3514.0
47	3736.0
48	4195.0
49	4689.0
50	5079.0
51	5657.0
52	6296.0
53	6432.0
54	6876.0
55	7399.0
56	7645.0
57	8250.0
58	8835.0
59	9591.0
60	10425.0
61	11497.0
62	12079.0
63	13123.0
64	14102.0
65	14938.0
66	16946.0
67	16751.0
68	17277.0
69	18509.0
70	21706.0
71	44875.0
72	127756.0
73	554796.0
74	1738225.0
75	5893019.0
76	1.5143284E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.315558399216975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.36267618676864	25.861123158083377
2	11.415072692572291	7.834291872265428
3	4.293347247405352	4.419858245893674
4	2.2095922969942983	3.0329357402387123
5	1.352574486995183	2.320717439888707
6	0.9019617542041142	1.8570792750150877
7	0.6426148760288757	1.5436181814602121
8	0.48834540088418543	1.340627609842423
9	0.36453872295106593	1.125841485258295
>10	2.4575632675457766	16.381472645288635
>50	0.2629235200746874	6.299332232196191
>100	0.2133339572121387	14.775404203997802
>500	0.024406358599948202	5.748370373917468
>1k	0.01081898751907218	5.97241957284975
>5k	1.5349616290505903E-4	0.357663282483368
>10k+	7.674808145251547E-5	1.1292446813209
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81696	0.34252334503126697	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	62871	0.26359656807506837	No Hit
CCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35851	0.15031096311589248	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	33969	0.14242038174900984	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.38531494886572E-6	0.0
2	0.0	0.0	0.0	8.38531494886572E-6	0.0
3	0.0	0.0	0.0	8.38531494886572E-6	0.0
4	0.0	0.0	0.0	8.38531494886572E-6	8.38531494886572E-6
5	0.0	0.0	0.0	8.38531494886572E-6	8.38531494886572E-6
6	4.19265747443286E-6	0.0	0.0	8.38531494886572E-6	8.38531494886572E-6
7	4.19265747443286E-6	0.0	0.0	3.773391726989574E-5	8.38531494886572E-6
8	4.19265747443286E-6	0.0	0.0	4.1926574744328605E-5	8.38531494886572E-6
9	4.19265747443286E-6	0.0	0.0	4.611923221876146E-5	8.38531494886572E-6
10	4.19265747443286E-6	0.0	0.0	4.611923221876146E-5	8.38531494886572E-6
11	8.38531494886572E-6	0.0	0.0	4.611923221876146E-5	8.38531494886572E-6
12	2.0963287372164302E-5	0.0	0.0	4.611923221876146E-5	8.38531494886572E-6
13	3.354125979546288E-5	0.0	0.0	4.611923221876146E-5	8.38531494886572E-6
14	4.611923221876146E-5	0.0	0.0	4.611923221876146E-5	8.38531494886572E-6
15	5.031188969319432E-5	0.0	0.0	4.611923221876146E-5	8.38531494886572E-6
16	5.4504547167627185E-5	0.0	0.0	4.611923221876146E-5	8.38531494886572E-6
17	5.4504547167627185E-5	0.0	0.0	4.611923221876146E-5	8.38531494886572E-6
18	5.4504547167627185E-5	0.0	0.0	4.611923221876146E-5	8.38531494886572E-6
19	5.4504547167627185E-5	0.0	0.0	4.611923221876146E-5	1.257797242329858E-5
20	5.869720464206004E-5	0.0	0.0	4.611923221876146E-5	1.257797242329858E-5
21	5.869720464206004E-5	0.0	0.0	4.611923221876146E-5	1.257797242329858E-5
22	6.28898621164929E-5	0.0	0.0	5.031188969319432E-5	1.257797242329858E-5
23	6.708251959092576E-5	4.19265747443286E-6	0.0	5.031188969319432E-5	1.257797242329858E-5
24	7.127517706535863E-5	4.19265747443286E-6	0.0	5.031188969319432E-5	1.257797242329858E-5
25	7.966049201422435E-5	4.19265747443286E-6	0.0	5.031188969319432E-5	1.257797242329858E-5
26	9.223846443752293E-5	4.19265747443286E-6	0.0	5.031188969319432E-5	1.257797242329858E-5
27	9.643112191195579E-5	4.19265747443286E-6	0.0	5.031188969319432E-5	1.257797242329858E-5
28	1.048164368608215E-4	4.19265747443286E-6	0.0	5.031188969319432E-5	1.257797242329858E-5
29	1.1739440928412009E-4	8.38531494886572E-6	0.0	5.031188969319432E-5	1.257797242329858E-5
30	1.257797242329858E-4	8.38531494886572E-6	0.0	5.4504547167627185E-5	1.257797242329858E-5
31	1.383576966562844E-4	8.38531494886572E-6	0.0	5.4504547167627185E-5	1.257797242329858E-5
32	1.5093566907958296E-4	8.38531494886572E-6	0.0	5.4504547167627185E-5	1.257797242329858E-5
33	1.593209840284487E-4	8.38531494886572E-6	0.0	5.4504547167627185E-5	1.257797242329858E-5
34	1.6770629897731442E-4	1.257797242329858E-5	0.0	5.869720464206004E-5	1.257797242329858E-5
35	1.7189895645174728E-4	1.257797242329858E-5	0.0	5.869720464206004E-5	1.677062989773144E-5
36	1.8028427140061299E-4	1.257797242329858E-5	0.0	6.708251959092576E-5	1.677062989773144E-5
37	1.9705490129834444E-4	1.257797242329858E-5	0.0	6.708251959092576E-5	1.677062989773144E-5
38	2.0544021624721015E-4	1.257797242329858E-5	0.0	6.708251959092576E-5	2.515594484659716E-5
39	2.222108461449416E-4	1.257797242329858E-5	0.0	6.708251959092576E-5	2.515594484659716E-5
40	2.2640350361937444E-4	1.257797242329858E-5	0.0	7.127517706535863E-5	2.515594484659716E-5
41	2.431741335171059E-4	1.257797242329858E-5	0.0	7.127517706535863E-5	2.515594484659716E-5
42	2.5994476341483733E-4	1.257797242329858E-5	0.0	7.546783453979148E-5	2.515594484659716E-5
43	2.6833007836370306E-4	1.257797242329858E-5	0.0	8.804580696309006E-5	2.515594484659716E-5
44	2.934860232103002E-4	1.257797242329858E-5	0.0	9.643112191195579E-5	3.773391726989574E-5
45	3.060639956335988E-4	1.257797242329858E-5	0.0	1.0062377938638864E-4	3.773391726989574E-5
46	3.1025665310803165E-4	1.257797242329858E-5	0.0	1.0900909433525437E-4	3.773391726989574E-5
47	3.186419680568974E-4	1.257797242329858E-5	0.0	1.0900909433525437E-4	3.773391726989574E-5
48	3.4379791290349457E-4	1.257797242329858E-5	0.0	1.1320175180968722E-4	3.773391726989574E-5
49	3.689538577500917E-4	1.257797242329858E-5	0.0	1.1739440928412009E-4	3.773391726989574E-5
50	3.8153183017339027E-4	1.257797242329858E-5	0.0	1.1739440928412009E-4	3.773391726989574E-5
51	4.0668777501998745E-4	1.257797242329858E-5	0.0	1.257797242329858E-4	3.773391726989574E-5
52	4.360363773410175E-4	1.257797242329858E-5	0.0	1.3416503918185153E-4	3.773391726989574E-5
53	4.6957763713648034E-4	1.257797242329858E-5	0.0	1.3416503918185153E-4	3.773391726989574E-5
54	4.821556095597789E-4	1.257797242329858E-5	0.0	1.383576966562844E-4	3.773391726989574E-5
55	5.031188969319432E-4	1.257797242329858E-5	0.0	1.467430116051501E-4	3.773391726989574E-5
56	5.282748417785404E-4	1.257797242329858E-5	0.0	1.467430116051501E-4	3.773391726989574E-5
57	5.576234440995704E-4	1.257797242329858E-5	0.0	1.5093566907958296E-4	3.773391726989574E-5
58	5.99550018843899E-4	1.257797242329858E-5	0.0	1.5093566907958296E-4	3.773391726989574E-5
59	6.582472234859591E-4	1.257797242329858E-5	0.0	1.593209840284487E-4	3.773391726989574E-5
60	6.959811407558548E-4	1.257797242329858E-5	0.0	1.6351364150288155E-4	3.773391726989574E-5
61	7.169444281280191E-4	1.257797242329858E-5	0.0	1.6770629897731442E-4	3.773391726989574E-5
62	7.379077155001834E-4	1.257797242329858E-5	0.0	1.6770629897731442E-4	3.773391726989574E-5
63	7.379077155001834E-4	1.257797242329858E-5	0.0	1.6770629897731442E-4	3.773391726989574E-5
64	7.379077155001834E-4	1.257797242329858E-5	0.0	1.6770629897731442E-4	3.773391726989574E-5
65	7.421003729746162E-4	1.677062989773144E-5	0.0	1.7189895645174728E-4	4.1926574744328605E-5
>>END_MODULE
