##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543825_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23851221
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.394985061771052	32.0	32.0	32.0	32.0	32.0
2	31.681252796240493	32.0	32.0	32.0	32.0	32.0
3	35.824142168654596	37.0	37.0	37.0	32.0	37.0
4	36.38047553204928	37.0	37.0	37.0	37.0	37.0
5	36.50732098788569	37.0	37.0	37.0	37.0	37.0
6	40.03930935024249	41.0	41.0	41.0	37.0	41.0
7	40.11396573785468	41.0	41.0	41.0	37.0	41.0
8	40.11232905015638	41.0	41.0	41.0	37.0	41.0
9	40.051316576203796	41.0	41.0	41.0	37.0	41.0
10-11	39.80728722441505	41.0	41.0	41.0	37.0	41.0
12-13	39.703953583759926	41.0	41.0	41.0	37.0	41.0
14-15	39.66169503439677	41.0	41.0	41.0	37.0	41.0
16-17	39.65375816609137	41.0	41.0	41.0	37.0	41.0
18-19	39.60405073182626	41.0	41.0	41.0	37.0	41.0
20-21	39.564818799004044	41.0	41.0	41.0	37.0	41.0
22-23	39.53117528029277	41.0	41.0	41.0	37.0	41.0
24-25	39.48103277396155	41.0	41.0	41.0	37.0	41.0
26-27	39.36622060145265	41.0	41.0	41.0	37.0	41.0
28-29	39.36218724399895	41.0	41.0	41.0	37.0	41.0
30-31	39.39434033586792	41.0	41.0	41.0	37.0	41.0
32-33	39.44621935707191	41.0	41.0	41.0	37.0	41.0
34-35	39.45654901692454	41.0	41.0	41.0	37.0	41.0
36-37	39.66169124650068	41.0	41.0	41.0	37.0	41.0
38-39	39.73651876795009	41.0	41.0	41.0	37.0	41.0
40-41	39.79438633226658	41.0	41.0	41.0	37.0	41.0
42-43	39.800537096264435	41.0	41.0	41.0	37.0	41.0
44-45	39.8069388460889	41.0	41.0	41.0	37.0	41.0
46-47	39.71434540260351	41.0	41.0	41.0	37.0	41.0
48-49	39.77101746455794	41.0	41.0	41.0	37.0	41.0
50-51	39.74948622577743	41.0	41.0	41.0	37.0	41.0
52-53	39.682470164376234	41.0	41.0	41.0	37.0	41.0
54-55	39.716800521822464	41.0	41.0	41.0	37.0	41.0
56-57	39.70446306819492	41.0	41.0	41.0	37.0	41.0
58-59	39.61064596992429	41.0	41.0	41.0	37.0	41.0
60-61	39.64173272135163	41.0	41.0	41.0	37.0	41.0
62-63	39.60486586989697	41.0	41.0	41.0	37.0	41.0
64-65	39.51350941457152	41.0	41.0	41.0	37.0	41.0
66-67	39.47039561396198	41.0	41.0	41.0	37.0	41.0
68-69	39.430568016713	41.0	41.0	41.0	37.0	41.0
70-71	39.37149324940175	41.0	41.0	41.0	37.0	41.0
72-73	39.26793708784376	41.0	41.0	41.0	37.0	41.0
74-75	39.18091281964274	41.0	41.0	41.0	37.0	41.0
76	38.451602032496936	41.0	37.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	63568.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	7.0
17	24.0
18	57.0
19	156.0
20	404.0
21	936.0
22	2288.0
23	4192.0
24	7352.0
25	12924.0
26	22610.0
27	36126.0
28	55599.0
29	81093.0
30	114887.0
31	158224.0
32	211049.0
33	279747.0
34	368153.0
35	488186.0
36	664909.0
37	978015.0
38	1709240.0
39	4907780.0
40	1.3683694E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64399673471602	10.012931440056354	11.938215605378733	36.40485621984889
2	22.93968825252243	14.44758099811839	32.99878147336439	29.613949275994788
3	22.47782494557155	19.23998975434861	26.793042592306186	31.48914270777365
4	27.363297843358442	26.102990884673055	20.423169686610606	26.110541585357893
5	26.63116869915666	29.27977383897436	23.981315012456253	20.107742449412726
6	21.87496032599817	31.61459461809081	26.655228783310715	19.85521627260031
7	18.86307993478802	21.965866073462564	41.36223947776605	17.808814513983368
8	21.270235646283304	21.777760532566504	33.19897903117767	23.753024789972525
9	21.388827220575312	21.566318459412535	34.70665643222557	22.338197887786578
10-11	24.326449944431257	29.214128438816555	25.93065822845154	20.528763388300643
12-13	23.993052584376017	23.457259712386108	29.943553877058505	22.60613382617937
14-15	23.176280612803414	25.292732397170358	30.020761660825247	21.51022532920098
16-17	23.519055154488626	25.53873831494297	29.27148049976054	21.67072603080787
18-19	23.500370990829275	25.800505724609195	29.07957280353461	21.61955048102692
20-21	23.17573404686886	25.690253135555636	29.448600429214316	21.68541238836119
22-23	23.628139243989963	25.461888064197602	28.972009291665522	21.937963400146913
24-25	23.50328510068064	25.573157177311188	28.91512254473478	22.008435177273398
26-27	23.24809025654445	25.855645328751507	28.9504423396913	21.945822075012735
28-29	23.636290975662025	25.633060185027322	28.856999653258242	21.87364918605241
30-31	23.320923902737892	25.756054621788323	28.762317807614192	22.160703667859593
32-33	23.25836992761539	25.514853334423215	28.998359459371727	22.228417278589667
34-35	23.38506571180663	25.631952656650625	28.830705609575645	22.152276021967097
36-37	23.73802164221446	25.456934265005987	28.56380108615426	22.24124300662529
38-39	23.360850126450732	25.8319399967942	28.832838436513843	21.974371440241224
40-41	23.678023190073798	25.358243480631188	28.872365414449796	22.091367914845215
42-43	23.310237949374443	25.159341277398173	29.25042169802729	22.279999075200095
44-45	23.46909269011366	25.22197553130619	29.246274708113436	22.06265707046672
46-47	23.453716096022962	25.442718124426587	28.978506574542134	22.125059205008313
48-49	23.48564239053615	25.35451427579091	28.82602138395186	22.333821949721084
50-51	23.27006604716865	25.506852005927016	28.981507125570964	22.241574821333362
52-53	23.472134155091855	25.337736303515403	28.768333679982415	22.421795861410327
54-55	23.346168971799237	25.563080775883396	28.572910505620648	22.517839746696726
56-57	23.578655344079866	25.28563648916431	28.896095045996955	22.23961312075887
58-59	23.5164480613837	25.216300852282554	28.827159000312424	22.440092086021327
60-61	23.204810231757744	25.277563186882496	28.93636342391875	22.581263157441015
62-63	23.097200020473313	25.499123750609655	29.0944576961684	22.30921853274863
64-65	23.509742825518913	25.434130518130456	28.730304164917424	22.325822491433208
66-67	23.591480552616357	25.289698188040262	28.641221311902363	22.47759994744102
68-69	23.318122610215113	25.673835798409684	28.709248454237436	22.298793137137764
70-71	23.60310659753931	25.47730247828841	28.593419530749454	22.32617139342283
72-73	23.514275480192175	25.142219904523376	28.74945132429402	22.594053290990423
74-75	23.647088205232283	22.032287045453042	30.34987758984068	23.970747159473998
76	24.532195205666056	0.0	41.810277879485234	33.65752691484871
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	326350.0
1	214297.5
2	66270.0
3	28512.5
4	26730.0
5	31363.5
6	45074.0
7	63372.5
8	72590.0
9	75744.5
10	83552.0
11	83956.5
12	79715.5
13	62341.5
14	30269.0
15	13167.0
16	10280.5
17	10210.5
18	11456.5
19	15007.5
20	20079.5
21	24371.5
22	30886.5
23	41654.0
24	54721.0
25	67138.0
26	93131.5
27	133257.5
28	171060.0
29	204392.5
30	255907.0
31	319576.5
32	380376.0
33	429408.0
34	494046.0
35	594602.5
36	680337.5
37	720208.5
38	731647.0
39	772092.5
40	833929.0
41	876729.0
42	899653.0
43	944336.5
44	1021825.0
45	1106423.0
46	1153210.0
47	1190819.5
48	1220105.0
49	1219047.0
50	1216548.0
51	1209866.5
52	1212811.0
53	1180558.0
54	1141796.5
55	1087635.0
56	989694.5
57	882899.5
58	806571.5
59	726340.0
60	619726.0
61	509819.0
62	427680.5
63	365229.5
64	288676.5
65	226779.5
66	167984.0
67	134363.5
68	109650.5
69	80496.0
70	62053.5
71	52009.5
72	44862.5
73	36897.0
74	29343.0
75	23512.0
76	20838.5
77	16989.0
78	13916.0
79	13353.5
80	11566.0
81	10325.5
82	9296.5
83	7446.5
84	6355.5
85	5309.5
86	4959.0
87	4791.0
88	3231.5
89	1394.0
90	621.0
91	234.5
92	140.0
93	110.0
94	74.5
95	65.0
96	60.5
97	42.5
98	14.5
99	5.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.079865051772402
2	0.3246290829303875
3	0.2665188503347481
4	0.273985973296713
5	0.2665188503347481
6	0.2666655933463532
7	0.2665188503347481
8	0.2667326758659441
9	0.2665188503347481
10-11	0.2665188503347481
12-13	0.2669485977258774
14-15	0.26675783181079077
16-17	0.26677250611195125
18-19	0.26653142830717136
20-21	0.26653142830717136
22-23	0.26693182709597973
24-25	0.2685418075661619
26-27	0.277637777956944
28-29	0.28069841791328
30-31	0.28157468332543645
32-33	0.28852610941804613
34-35	0.2958464893684059
36-37	4.77316329937869E-4
38-39	0.0015267479939666956
40-41	3.6807502336487665E-4
42-43	6.311190940664076E-6
44-45	0.0
46-47	2.946699985424359E-5
48-49	6.758512536030135E-4
50-51	4.80231977320413E-4
52-53	2.1915760284908257E-4
54-55	1.4759078910343108E-4
56-57	0.001660367572468557
58-59	0.0020056484126200636
60-61	1.2677541574518546E-5
62-63	0.0027198312029610375
64-65	0.0
66-67	0.0012656224878347603
68-69	0.001216452423494855
70-71	0.0
72-73	2.5634054054700683E-5
74-75	6.438541581461788E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	71888.0
36	1106.0
37	1382.0
38	1638.0
39	1804.0
40	2151.0
41	2562.0
42	2770.0
43	2797.0
44	2990.0
45	2998.0
46	3495.0
47	3720.0
48	4182.0
49	4677.0
50	5067.0
51	5632.0
52	6290.0
53	6413.0
54	6884.0
55	7386.0
56	7635.0
57	8227.0
58	8826.0
59	9593.0
60	10428.0
61	11481.0
62	12052.0
63	13097.0
64	14064.0
65	14635.0
66	16238.0
67	16413.0
68	17215.0
69	18339.0
70	20576.0
71	45249.0
72	105914.0
73	443597.0
74	1710252.0
75	5490014.0
76	1.5709544E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.695303622711478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.53810922771021	20.24112461261131
2	13.34570051484345	7.659178566624194
3	5.14771279958223	4.431455452395905
4	2.7614494159530385	3.169625177181269
5	1.6054954780125208	2.30350901032298
6	1.0901777951773672	1.876978970121163
7	0.785852414848094	1.5785191552694953
8	0.602214798921624	1.3824589200916912
9	0.4630999233340981	1.1959913616953717
>10	3.025180425969428	16.760287699656644
>50	0.33636183993295543	6.7259418573299135
>100	0.25360400390539384	14.538807694242648
>500	0.028681723659236633	5.724199874420148
>1k	0.01585441972099981	8.084238006876749
>5k	2.6026403915075573E-4	0.49324974386160786
>10k+	2.4495438978894377E-4	3.8344338972988994
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	236969	0.9935298490588804	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	85459	0.35830031510755783	No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	80252	0.33646914763818586	No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71803	0.30104538463670266	No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69091	0.28967489756604076	No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67933	0.2848198002106475	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	63568	0.2665188503347481	No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49944	0.20939808490307477	No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30208	0.12665179698766785	No Hit
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29239	0.12258911189494241	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.38531494886572E-6	0.0
2	0.0	0.0	0.0	8.38531494886572E-6	4.19265747443286E-6
3	0.0	0.0	0.0	8.38531494886572E-6	1.677062989773144E-5
4	2.0963287372164302E-5	0.0	0.0	8.38531494886572E-6	1.677062989773144E-5
5	2.0963287372164302E-5	0.0	0.0	1.257797242329858E-5	2.515594484659716E-5
6	2.934860232103002E-5	0.0	0.0	1.257797242329858E-5	2.515594484659716E-5
7	3.773391726989574E-5	0.0	0.0	1.257797242329858E-5	2.515594484659716E-5
8	4.611923221876146E-5	0.0	0.0	1.257797242329858E-5	2.515594484659716E-5
9	5.4504547167627185E-5	0.0	4.19265747443286E-6	1.257797242329858E-5	2.934860232103002E-5
10	7.127517706535863E-5	0.0	4.19265747443286E-6	1.257797242329858E-5	2.934860232103002E-5
11	9.643112191195579E-5	0.0	4.19265747443286E-6	1.257797242329858E-5	2.934860232103002E-5
12	1.0062377938638864E-4	0.0	4.19265747443286E-6	1.257797242329858E-5	2.934860232103002E-5
13	1.0062377938638864E-4	0.0	4.19265747443286E-6	1.257797242329858E-5	3.773391726989574E-5
14	1.0062377938638864E-4	0.0	4.19265747443286E-6	1.257797242329858E-5	4.1926574744328605E-5
15	1.048164368608215E-4	0.0	4.19265747443286E-6	1.677062989773144E-5	4.611923221876146E-5
16	1.0900909433525437E-4	0.0	4.19265747443286E-6	2.0963287372164302E-5	4.611923221876146E-5
17	1.1320175180968722E-4	0.0	4.19265747443286E-6	2.0963287372164302E-5	5.4504547167627185E-5
18	1.1739440928412009E-4	0.0	4.19265747443286E-6	2.0963287372164302E-5	6.708251959092576E-5
19	1.1739440928412009E-4	4.19265747443286E-6	4.19265747443286E-6	2.0963287372164302E-5	6.708251959092576E-5
20	1.1739440928412009E-4	4.19265747443286E-6	4.19265747443286E-6	2.0963287372164302E-5	6.708251959092576E-5
21	1.1739440928412009E-4	4.19265747443286E-6	4.19265747443286E-6	2.0963287372164302E-5	7.127517706535863E-5
22	1.1739440928412009E-4	4.19265747443286E-6	4.19265747443286E-6	2.934860232103002E-5	7.127517706535863E-5
23	1.2997238170741867E-4	4.19265747443286E-6	4.19265747443286E-6	3.354125979546288E-5	7.127517706535863E-5
24	1.383576966562844E-4	4.19265747443286E-6	4.19265747443286E-6	3.354125979546288E-5	7.127517706535863E-5
25	1.593209840284487E-4	4.19265747443286E-6	4.19265747443286E-6	3.354125979546288E-5	7.127517706535863E-5
26	1.7609161392618012E-4	4.19265747443286E-6	4.19265747443286E-6	3.354125979546288E-5	7.127517706535863E-5
27	1.8028427140061299E-4	4.19265747443286E-6	4.19265747443286E-6	3.354125979546288E-5	7.966049201422435E-5
28	1.9286224382391158E-4	4.19265747443286E-6	4.19265747443286E-6	3.354125979546288E-5	7.966049201422435E-5
29	1.9705490129834444E-4	4.19265747443286E-6	4.19265747443286E-6	4.1926574744328605E-5	8.385314948865721E-5
30	2.0544021624721015E-4	4.19265747443286E-6	4.19265747443286E-6	4.1926574744328605E-5	8.385314948865721E-5
31	2.0544021624721015E-4	4.19265747443286E-6	4.19265747443286E-6	4.611923221876146E-5	8.385314948865721E-5
32	2.0544021624721015E-4	1.257797242329858E-5	4.19265747443286E-6	5.031188969319432E-5	8.385314948865721E-5
33	2.431741335171059E-4	1.677062989773144E-5	4.19265747443286E-6	5.031188969319432E-5	8.804580696309006E-5
34	2.431741335171059E-4	2.0963287372164302E-5	4.19265747443286E-6	5.031188969319432E-5	9.223846443752293E-5
35	2.431741335171059E-4	2.0963287372164302E-5	4.19265747443286E-6	5.4504547167627185E-5	9.223846443752293E-5
36	2.431741335171059E-4	2.0963287372164302E-5	4.19265747443286E-6	5.4504547167627185E-5	1.0062377938638864E-4
37	2.515594484659716E-4	2.0963287372164302E-5	4.19265747443286E-6	6.28898621164929E-5	1.048164368608215E-4
38	2.515594484659716E-4	2.0963287372164302E-5	4.19265747443286E-6	6.708251959092576E-5	1.1739440928412009E-4
39	2.515594484659716E-4	2.0963287372164302E-5	4.19265747443286E-6	7.127517706535863E-5	1.2158706675855295E-4
40	2.6833007836370306E-4	2.0963287372164302E-5	4.19265747443286E-6	7.127517706535863E-5	1.3416503918185153E-4
41	2.767153933125688E-4	2.0963287372164302E-5	4.19265747443286E-6	7.966049201422435E-5	1.383576966562844E-4
42	3.060639956335988E-4	2.0963287372164302E-5	4.19265747443286E-6	9.643112191195579E-5	1.5093566907958296E-4
43	3.060639956335988E-4	2.0963287372164302E-5	4.19265747443286E-6	1.048164368608215E-4	1.593209840284487E-4
44	3.186419680568974E-4	2.0963287372164302E-5	8.38531494886572E-6	1.1739440928412009E-4	1.6351364150288155E-4
45	3.3121994048019595E-4	2.0963287372164302E-5	8.38531494886572E-6	1.2158706675855295E-4	1.6351364150288155E-4
46	3.7733917269895743E-4	2.0963287372164302E-5	8.38531494886572E-6	1.3416503918185153E-4	1.6351364150288155E-4
47	3.89917145122256E-4	2.0963287372164302E-5	8.38531494886572E-6	1.5093566907958296E-4	1.6351364150288155E-4
48	3.89917145122256E-4	2.0963287372164302E-5	8.38531494886572E-6	1.5093566907958296E-4	1.6770629897731442E-4
49	3.89917145122256E-4	2.0963287372164302E-5	8.38531494886572E-6	1.593209840284487E-4	1.6770629897731442E-4
50	3.983024600711217E-4	2.0963287372164302E-5	8.38531494886572E-6	1.6770629897731442E-4	1.9286224382391158E-4
51	4.150730899688532E-4	2.0963287372164302E-5	8.38531494886572E-6	1.6770629897731442E-4	1.9705490129834444E-4
52	4.150730899688532E-4	2.0963287372164302E-5	8.38531494886572E-6	1.6770629897731442E-4	1.9705490129834444E-4
53	4.2765106239215175E-4	2.0963287372164302E-5	8.38531494886572E-6	1.7189895645174728E-4	2.0124755877277728E-4
54	4.318437198665846E-4	2.0963287372164302E-5	8.38531494886572E-6	1.7609161392618012E-4	2.0124755877277728E-4
55	4.444216922898832E-4	2.934860232103002E-5	8.38531494886572E-6	1.8028427140061299E-4	2.0124755877277728E-4
56	4.528070072387489E-4	2.934860232103002E-5	8.38531494886572E-6	1.8028427140061299E-4	2.0124755877277728E-4
57	4.569996647131818E-4	3.354125979546288E-5	8.38531494886572E-6	1.8028427140061299E-4	2.0544021624721015E-4
58	4.863482670342118E-4	3.354125979546288E-5	8.38531494886572E-6	1.8447692887504585E-4	2.09632873721643E-4
59	5.156968693552418E-4	3.354125979546288E-5	8.38531494886572E-6	2.0124755877277728E-4	2.09632873721643E-4
60	5.282748417785404E-4	3.354125979546288E-5	8.38531494886572E-6	2.0124755877277728E-4	2.09632873721643E-4
61	5.282748417785404E-4	3.354125979546288E-5	8.38531494886572E-6	2.0544021624721015E-4	2.09632873721643E-4
62	5.366601567274061E-4	3.354125979546288E-5	8.38531494886572E-6	2.0544021624721015E-4	2.09632873721643E-4
63	5.450454716762718E-4	3.773391726989574E-5	8.38531494886572E-6	2.09632873721643E-4	2.1382553119607588E-4
64	5.450454716762718E-4	4.1926574744328605E-5	8.38531494886572E-6	2.09632873721643E-4	2.1382553119607588E-4
65	5.492381291507047E-4	4.611923221876146E-5	1.257797242329858E-5	2.1382553119607588E-4	2.1801818867050874E-4
>>END_MODULE
