##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543821_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26776805
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.523821045864135	32.0	32.0	32.0	32.0	32.0
2	31.689453054612006	32.0	32.0	32.0	32.0	32.0
3	35.93074341767063	37.0	37.0	37.0	32.0	37.0
4	36.41059342218013	37.0	37.0	37.0	37.0	37.0
5	36.55370299033062	37.0	37.0	37.0	37.0	37.0
6	40.09399758485002	41.0	41.0	41.0	37.0	41.0
7	40.22206865232801	41.0	41.0	41.0	37.0	41.0
8	40.15941913159542	41.0	41.0	41.0	37.0	41.0
9	40.08258602921446	41.0	41.0	41.0	37.0	41.0
10-11	39.81954021400238	41.0	41.0	41.0	37.0	41.0
12-13	39.7214909134977	41.0	41.0	41.0	37.0	41.0
14-15	39.78641437990828	41.0	41.0	41.0	37.0	41.0
16-17	39.73088247458948	41.0	41.0	41.0	37.0	41.0
18-19	39.690838059283024	41.0	41.0	41.0	37.0	41.0
20-21	39.660912252227256	41.0	41.0	41.0	37.0	41.0
22-23	39.63904011326221	41.0	41.0	41.0	37.0	41.0
24-25	39.59832967376056	41.0	41.0	41.0	37.0	41.0
26-27	39.478109972418295	41.0	41.0	41.0	37.0	41.0
28-29	39.48837363158151	41.0	41.0	41.0	37.0	41.0
30-31	39.53290433642102	41.0	41.0	41.0	37.0	41.0
32-33	39.58259732630536	41.0	41.0	41.0	37.0	41.0
34-35	39.58126514720483	41.0	41.0	41.0	37.0	41.0
36-37	39.73748103451819	41.0	41.0	41.0	37.0	41.0
38-39	39.8106954760555	41.0	41.0	41.0	37.0	41.0
40-41	39.88213239747159	41.0	41.0	41.0	37.0	41.0
42-43	39.896473738807614	41.0	41.0	41.0	37.0	41.0
44-45	39.91175662831709	41.0	41.0	41.0	37.0	41.0
46-47	39.82740287210408	41.0	41.0	41.0	37.0	41.0
48-49	39.90232609605027	41.0	41.0	41.0	37.0	41.0
50-51	39.89228854630659	41.0	41.0	41.0	37.0	41.0
52-53	39.83719479758145	41.0	41.0	41.0	37.0	41.0
54-55	39.875398255667015	41.0	41.0	41.0	37.0	41.0
56-57	39.874079254146245	41.0	41.0	41.0	37.0	41.0
58-59	39.8028530495001	41.0	41.0	41.0	37.0	41.0
60-61	39.83954760698323	41.0	41.0	41.0	37.0	41.0
62-63	39.82364964949585	41.0	41.0	41.0	37.0	41.0
64-65	39.763135761127536	41.0	41.0	41.0	37.0	41.0
66-67	39.75842119492957	41.0	41.0	41.0	37.0	41.0
68-69	39.74636242951213	41.0	41.0	41.0	37.0	41.0
70-71	39.73167586877703	41.0	41.0	41.0	37.0	41.0
72-73	39.68316290616599	41.0	41.0	41.0	37.0	41.0
74-75	39.66350919316958	41.0	41.0	41.0	37.0	41.0
76	39.187928964250986	41.0	41.0	41.0	37.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20988.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	4.0
16	11.0
17	17.0
18	71.0
19	134.0
20	405.0
21	1057.0
22	2539.0
23	4637.0
24	8040.0
25	13467.0
26	22145.0
27	35508.0
28	54362.0
29	79652.0
30	113604.0
31	156792.0
32	212955.0
33	283916.0
34	376441.0
35	501812.0
36	687549.0
37	996333.0
38	1698392.0
39	4913802.0
40	1.6592171E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.84773646174667	10.166868508537807	12.134939807709063	35.85045522200646
2	23.193052028783992	14.756780096016941	33.54056053435853	28.509607340840542
3	22.682536661093174	19.474004475363245	27.33799906016699	30.50545980337659
4	27.532132675827743	26.315907312150056	20.785344429609193	25.36661558241301
5	26.804526283013523	29.375055151558254	24.262043652040227	19.558374913387993
6	22.268038717182826	31.85288376499289	26.570729475202153	19.308348042622132
7	18.962448427569974	22.003775851808225	41.79984860862219	17.23392711199961
8	21.49101324518286	21.932559207333448	33.35052838145349	23.2258991660302
9	21.670723043142356	21.35984485168216	34.997499796025664	21.971932309149818
10-11	24.418376011466965	29.321137904329365	26.087904548009128	20.17258153619454
12-13	24.276051566424865	23.348733414003984	30.10440689581556	22.270808123755593
14-15	23.18817244630044	25.34026783988689	30.275659137484446	21.195900576328217
16-17	23.62650162598712	25.580621987493007	29.525531468061793	21.26734491845808
18-19	23.627130604718857	25.83742221106452	29.36908085414783	21.16636633006879
20-21	23.178946197598258	25.738205002404523	29.694051905655044	21.388796894342175
22-23	23.63939065846925	25.383721957834844	29.20187361418385	21.775013769512057
24-25	23.61451533171924	25.56076605111248	29.13606356539626	21.688655051772017
26-27	23.322097972846816	25.85634128901717	29.171482217204	21.650078520932006
28-29	23.81695614208219	25.527455307747054	29.143987852798166	21.511600697372593
30-31	23.51000380915925	25.724196310386294	28.94729828184115	21.818501598613302
32-33	23.40203062811897	25.451971537373435	29.134046970717254	22.011950863790343
34-35	23.434604689781455	25.73601751050345	29.037343667034833	21.792034132680257
36-37	24.002745028235214	25.32637405696676	28.693064402776784	21.97781651202124
38-39	23.35850377818446	25.8338776817163	29.053785546837567	21.753832993261675
40-41	23.81759685137582	25.32453972881536	29.09050181272623	21.76736160708258
42-43	23.358128508303395	25.090620824617428	29.512817553539826	22.038433113539355
44-45	23.577399812207382	25.27139326425651	29.391904224080356	21.759302699455755
46-47	23.524956034535162	25.421472948625162	29.284645280371358	21.768925736468322
48-49	23.57548818549394	25.31029281619262	29.118068902737924	21.99615009557552
50-51	23.304151756510354	25.558453709109134	29.16175144747497	21.975643086905546
52-53	23.607092518868825	25.242970053219203	29.04222349772885	22.10771393018312
54-55	23.442640957683096	25.56930248464778	28.66201751294429	22.326039044724837
56-57	23.79142686821436	25.177562814947972	29.116319105934846	21.914691210902816
58-59	23.72422127667882	25.18522537018888	28.991767365036843	22.098785988095464
60-61	23.1611780606343	25.279915657765024	29.248439331641663	22.31046694995901
62-63	23.162091279748395	25.492293436329444	29.351528231640316	21.994087052281845
64-65	23.56683662643161	25.34640198210395	29.011055659925177	22.07570573153926
66-67	23.74250612839453	25.230973184453465	28.908952065074683	22.117568622077325
68-69	23.357869516480417	25.67910592336225	29.01409380256224	21.948930757595093
70-71	23.686055676987454	25.388097437089797	28.86576751514819	22.060079370774563
72-73	23.621765274531082	25.023619127432077	29.039796882335907	22.31481871570093
74-75	23.71167752700523	21.9204856776148	30.541967219726402	23.82586957565357
76	24.576521405816006	0.0	42.12332495910487	33.30015363507912
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	253104.0
1	188113.0
2	78445.0
3	31190.5
4	28613.5
5	33162.5
6	47554.0
7	67860.5
8	78326.0
9	77629.0
10	87208.5
11	84972.5
12	72464.0
13	60314.5
14	32363.0
15	14192.0
16	11140.0
17	10728.5
18	11811.5
19	15652.5
20	21215.0
21	25735.0
22	32736.0
23	44074.5
24	58070.0
25	71997.0
26	99352.5
27	147399.0
28	202007.0
29	248038.0
30	305037.0
31	366453.5
32	433851.0
33	498433.0
34	578775.0
35	691765.5
36	793836.5
37	842954.0
38	857690.0
39	917954.0
40	994128.5
41	1027868.0
42	1042737.0
43	1120971.0
44	1225875.0
45	1298528.5
46	1328346.5
47	1387518.0
48	1435166.0
49	1409301.0
50	1386169.0
51	1385138.5
52	1397240.0
53	1340123.5
54	1281456.5
55	1207900.0
56	1078804.0
57	938589.5
58	834556.0
59	745293.0
60	626989.5
61	507722.5
62	422502.0
63	359675.0
64	283128.5
65	223587.0
66	165310.5
67	130699.0
68	103987.0
69	73839.0
70	58133.5
71	50054.5
72	46180.5
73	39864.5
74	32013.0
75	25808.0
76	19566.5
77	13862.5
78	11708.5
79	12092.0
80	10195.0
81	8405.5
82	7508.5
83	6301.0
84	6226.0
85	5456.5
86	4985.0
87	5312.0
88	3624.5
89	1617.5
90	862.5
91	419.5
92	238.5
93	217.0
94	178.5
95	169.0
96	175.5
97	120.0
98	45.5
99	17.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9007086543745604
2	0.13484431768465283
3	0.0783812706557037
4	0.08576079184951303
5	0.0783812706557037
6	0.0785605302798448
7	0.0783812706557037
8	0.07857546858185657
9	0.0783812706557037
10-11	0.0783812706557037
12-13	0.07880327768753591
14-15	0.07859040688386834
16-17	0.07864269094090949
18-19	0.078394341669964
20-21	0.07839620895771546
22-23	0.07893585511789028
24-25	0.0807377877980588
26-27	0.08987629405375286
28-29	0.09323367743089589
30-31	0.09521300244745406
32-33	0.1034197321151646
34-35	0.11161525805636631
36-37	5.047769378559577E-4
38-39	0.0013649425354518114
40-41	3.3288029198314293E-4
42-43	9.352669041309336E-6
44-45	0.0
46-47	3.3687131811346226E-5
48-49	6.571101385673048E-4
50-51	5.468694765831708E-4
52-53	1.9861438733766575E-4
54-55	1.6310337656985126E-4
56-57	0.0017708226135098233
58-59	0.002057318623805512
60-61	1.3149824649906113E-5
62-63	0.0026868450168943134
64-65	0.0
66-67	0.0010533273244055772
68-69	0.0013018341787016834
70-71	1.8894024305688533E-6
72-73	2.8465917614510556E-5
74-75	5.741399162456777E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	31600.0
36	1437.0
37	1701.0
38	2029.0
39	2405.0
40	2590.0
41	3064.0
42	3283.0
43	3385.0
44	3367.0
45	3537.0
46	3948.0
47	4192.0
48	4831.0
49	5217.0
50	5613.0
51	6272.0
52	6919.0
53	7361.0
54	7704.0
55	7910.0
56	8322.0
57	8833.0
58	9344.0
59	10137.0
60	10959.0
61	12005.0
62	12625.0
63	13736.0
64	14344.0
65	14979.0
66	16389.0
67	16953.0
68	17486.0
69	18685.0
70	20499.0
71	46524.0
72	118645.0
73	499014.0
74	1938454.0
75	6133850.0
76	1.7716657E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.972593007512653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.08219073039461	16.80051835454626
2	13.4372589839075	6.44251881515515
3	5.080916051034464	3.6540819779036275
4	2.6893986528117546	2.5788743735523605
5	1.5939539054608531	1.9105604124174165
6	1.1006201279920267	1.5830831030537607
7	0.8018995771663999	1.3456528537414616
8	0.6161861497424774	1.1817263827714128
9	0.4748412896212793	1.0244859281328034
>10	3.3335880366862107	15.708522019038696
>50	0.3964775917921096	6.6518266907994965
>100	0.321366082798339	15.492991100519163
>500	0.04174933912944367	6.948932908897263
>1k	0.027526006897341287	12.316849561642405
>5k	0.0015989027061315492	2.5147894416212475
>10k+	4.285718593754595E-4	3.8445860762075608
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	207966	0.7766647290444099	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107338	0.4008618653345685	No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	90526	0.338076181979142	No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73182	0.27330370445615154	No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67891	0.2535440654700962	No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59566	0.22245372440812114	No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52812	0.1972304014612647	No Hit
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32399	0.12099651171975148	No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31649	0.11819558009254652	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7345755029399513E-6	1.4938302011759805E-5	0.0	0.0	0.0
2	3.7345755029399513E-6	1.4938302011759805E-5	0.0	0.0	7.4691510058799025E-6
3	3.7345755029399513E-6	1.8672877514699756E-5	0.0	0.0	1.1203726508819854E-5
4	3.7345755029399513E-6	1.8672877514699756E-5	0.0	0.0	1.4938302011759805E-5
5	3.7345755029399513E-6	1.8672877514699756E-5	0.0	0.0	1.8672877514699756E-5
6	3.7345755029399513E-6	1.8672877514699756E-5	0.0	0.0	1.8672877514699756E-5
7	7.4691510058799025E-6	1.8672877514699756E-5	0.0	0.0	2.240745301763971E-5
8	1.8672877514699756E-5	1.8672877514699756E-5	0.0	1.4938302011759805E-5	2.240745301763971E-5
9	1.8672877514699756E-5	1.8672877514699756E-5	0.0	1.8672877514699756E-5	2.6142028520579658E-5
10	1.8672877514699756E-5	2.240745301763971E-5	0.0	2.240745301763971E-5	2.6142028520579658E-5
11	2.987660402351961E-5	2.240745301763971E-5	1.1203726508819854E-5	2.6142028520579658E-5	2.6142028520579658E-5
12	3.734575502939951E-5	2.240745301763971E-5	1.1203726508819854E-5	2.987660402351961E-5	2.6142028520579658E-5
13	3.734575502939951E-5	2.240745301763971E-5	1.1203726508819854E-5	3.361117952645956E-5	2.987660402351961E-5
14	4.108033053233946E-5	2.240745301763971E-5	1.1203726508819854E-5	3.361117952645956E-5	2.987660402351961E-5
15	5.601863254409927E-5	2.240745301763971E-5	1.1203726508819854E-5	3.361117952645956E-5	2.987660402351961E-5
16	5.601863254409927E-5	2.6142028520579658E-5	1.1203726508819854E-5	3.361117952645956E-5	2.987660402351961E-5
17	5.601863254409927E-5	2.6142028520579658E-5	1.1203726508819854E-5	3.361117952645956E-5	2.987660402351961E-5
18	5.601863254409927E-5	2.6142028520579658E-5	1.4938302011759805E-5	3.361117952645956E-5	3.361117952645956E-5
19	5.975320804703922E-5	2.6142028520579658E-5	1.4938302011759805E-5	3.361117952645956E-5	3.361117952645956E-5
20	6.722235905291913E-5	2.6142028520579658E-5	1.4938302011759805E-5	3.361117952645956E-5	3.361117952645956E-5
21	6.722235905291913E-5	2.6142028520579658E-5	1.4938302011759805E-5	3.734575502939951E-5	3.361117952645956E-5
22	6.722235905291913E-5	2.6142028520579658E-5	1.4938302011759805E-5	4.108033053233946E-5	3.361117952645956E-5
23	6.722235905291913E-5	2.6142028520579658E-5	1.8672877514699756E-5	4.108033053233946E-5	3.361117952645956E-5
24	6.722235905291913E-5	2.6142028520579658E-5	1.8672877514699756E-5	4.108033053233946E-5	3.361117952645956E-5
25	6.722235905291913E-5	2.6142028520579658E-5	1.8672877514699756E-5	4.108033053233946E-5	3.361117952645956E-5
26	7.469151005879902E-5	2.6142028520579658E-5	1.8672877514699756E-5	4.108033053233946E-5	3.361117952645956E-5
27	7.842608556173898E-5	2.6142028520579658E-5	1.8672877514699756E-5	4.108033053233946E-5	3.361117952645956E-5
28	8.216066106467892E-5	2.6142028520579658E-5	1.8672877514699756E-5	4.108033053233946E-5	3.361117952645956E-5
29	8.216066106467892E-5	2.6142028520579658E-5	1.8672877514699756E-5	4.108033053233946E-5	3.734575502939951E-5
30	8.962981207055883E-5	2.6142028520579658E-5	1.8672877514699756E-5	4.108033053233946E-5	3.734575502939951E-5
31	9.709896307643873E-5	2.6142028520579658E-5	1.8672877514699756E-5	4.108033053233946E-5	4.108033053233946E-5
32	1.0083353857937869E-4	2.6142028520579658E-5	1.8672877514699756E-5	4.108033053233946E-5	4.108033053233946E-5
33	1.0456811408231863E-4	2.6142028520579658E-5	1.8672877514699756E-5	4.108033053233946E-5	4.481490603527942E-5
34	1.0830268958525859E-4	2.6142028520579658E-5	1.8672877514699756E-5	4.108033053233946E-5	4.8549481538219366E-5
35	1.0830268958525859E-4	2.6142028520579658E-5	1.8672877514699756E-5	4.108033053233946E-5	4.8549481538219366E-5
36	1.0830268958525859E-4	2.6142028520579658E-5	1.8672877514699756E-5	5.2284057041159315E-5	5.2284057041159315E-5
37	1.1577184059113848E-4	2.6142028520579658E-5	1.8672877514699756E-5	5.601863254409927E-5	5.2284057041159315E-5
38	1.1950641609407844E-4	2.6142028520579658E-5	1.8672877514699756E-5	5.601863254409927E-5	6.722235905291913E-5
39	1.1950641609407844E-4	2.6142028520579658E-5	1.8672877514699756E-5	5.601863254409927E-5	7.095693455585907E-5
40	1.2324099159701838E-4	2.6142028520579658E-5	1.8672877514699756E-5	5.975320804703922E-5	7.842608556173898E-5
41	1.2324099159701838E-4	2.6142028520579658E-5	1.8672877514699756E-5	6.348778354997917E-5	7.842608556173898E-5
42	1.381792936087782E-4	2.6142028520579658E-5	1.8672877514699756E-5	6.348778354997917E-5	7.842608556173898E-5
43	1.456484446146581E-4	2.6142028520579658E-5	1.8672877514699756E-5	6.348778354997917E-5	7.842608556173898E-5
44	1.4938302011759805E-4	2.6142028520579658E-5	1.8672877514699756E-5	6.348778354997917E-5	8.216066106467892E-5
45	1.53117595620538E-4	2.6142028520579658E-5	1.8672877514699756E-5	7.095693455585907E-5	9.336438757349878E-5
46	1.53117595620538E-4	2.6142028520579658E-5	1.8672877514699756E-5	8.589523656761888E-5	9.336438757349878E-5
47	1.6058674662641791E-4	2.6142028520579658E-5	1.8672877514699756E-5	8.962981207055883E-5	9.336438757349878E-5
48	1.755250486381777E-4	2.6142028520579658E-5	1.8672877514699756E-5	8.962981207055883E-5	9.336438757349878E-5
49	1.8672877514699755E-4	2.6142028520579658E-5	1.8672877514699756E-5	9.709896307643873E-5	9.709896307643873E-5
50	1.904633506499375E-4	2.6142028520579658E-5	1.8672877514699756E-5	9.709896307643873E-5	1.0083353857937869E-4
51	2.0166707715875738E-4	2.6142028520579658E-5	1.8672877514699756E-5	9.709896307643873E-5	1.0083353857937869E-4
52	2.1660537917051717E-4	2.6142028520579658E-5	2.240745301763971E-5	9.709896307643873E-5	1.0083353857937869E-4
53	2.3527825668521692E-4	2.6142028520579658E-5	2.240745301763971E-5	9.709896307643873E-5	1.0083353857937869E-4
54	2.3901283218815688E-4	2.6142028520579658E-5	2.240745301763971E-5	9.709896307643873E-5	1.0083353857937869E-4
55	2.4274740769109684E-4	2.6142028520579658E-5	2.240745301763971E-5	1.0083353857937869E-4	1.0083353857937869E-4
56	2.5021655869697675E-4	2.6142028520579658E-5	2.6142028520579658E-5	1.0830268958525859E-4	1.0083353857937869E-4
57	2.539511341999167E-4	2.987660402351961E-5	2.987660402351961E-5	1.1203726508819854E-4	1.0083353857937869E-4
58	3.0996976674401593E-4	2.987660402351961E-5	2.987660402351961E-5	1.1203726508819854E-4	1.1203726508819854E-4
59	3.5851924828223533E-4	2.987660402351961E-5	2.987660402351961E-5	1.1203726508819854E-4	1.1577184059113848E-4
60	3.697229747910552E-4	2.987660402351961E-5	3.361117952645956E-5	1.1577184059113848E-4	1.1577184059113848E-4
61	3.771921257969351E-4	2.987660402351961E-5	3.361117952645956E-5	1.2324099159701838E-4	1.1577184059113848E-4
62	3.771921257969351E-4	2.987660402351961E-5	3.361117952645956E-5	1.2697556709995834E-4	1.1950641609407844E-4
63	3.80926701299875E-4	2.987660402351961E-5	3.361117952645956E-5	1.2697556709995834E-4	1.1950641609407844E-4
64	3.80926701299875E-4	3.361117952645956E-5	3.361117952645956E-5	1.3444471810583825E-4	1.1950641609407844E-4
65	3.84661276802815E-4	3.734575502939951E-5	3.734575502939951E-5	1.381792936087782E-4	1.2324099159701838E-4
>>END_MODULE
